Search

CN-121992010-A - Method for synergistically improving soybean yield and seed quality

CN121992010ACN 121992010 ACN121992010 ACN 121992010ACN-121992010-A

Abstract

The application belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to a method for synergistically improving soybean yield and seed quality. The application provides a method capable of synergistically improving soybean yield and seed quality, which comprises the steps of using a gene editing technology to simultaneously target and edit soybean cytokinin oxidase/dehydrogenase genes GmCKX and GmCKX, generating frame shift mutation and leading to early termination of protein translation. According to the method, two key cytokinin oxidase genes are knocked out simultaneously, so that the negative correlation between the yield and the protein content in traditional soybean breeding is broken, synchronous improvement of the yield and the seed protein content is realized, and meanwhile, the stability of the oil content is maintained.

Inventors

  • LI HONGJU
  • YANG WEICAI
  • YANG YILIN

Assignees

  • 中国科学院遗传与发育生物学研究所

Dates

Publication Date
20260508
Application Date
20251218

Claims (10)

  1. 1. A method for synergistically improving soybean yield and seed quality comprising: Performing gene editing on a target region on a second exon of a target gene of soybean to construct a soybean GmCKX/GmCKX mutant; the target region comprises a target region shown in SEQ ID NO.1 and a target region shown in SEQ ID NO. 2.
  2. 2. The method of claim 1, wherein the soybean GmCKX/GmCKX mutant has deletion mutations and/or insertion mutations in the target region.
  3. 3. The method of claim 2, wherein the soybean GmCKX/GmCKX mutant inserts a single base in the target region of SEQ ID No. 1.
  4. 4. The method of claim 2, wherein the soybean GmCKX/GmCKX mutant lacks multiple bases in the target region of SEQ ID No. 2.
  5. 5. The method of claim 2, wherein the soybean GmCKX/GmCKX mutant lacks 3-5 bases in the target region of SEQ ID No. 2.
  6. 6. The method of any one of claims 1 to 5, wherein the soybean GmCKX/GmCKX mutant has the fragments shown as SEQ ID No.5 and SEQ ID No. 7.
  7. 7. The method of claim 6, wherein gene editing comprises employing one or more of CRISPR/Cas9, CRISPR/Cpf1, CRISPR/Cas12i/j, TALEN, ZFN and back-complementing the relevant target gene knockdown.
  8. 8. The method of claim 7, wherein gene editing uses CRISPR/Cas9 technology.
  9. 9. The method of any one of claims 1 to 5, wherein the soybean variety comprises Williams 82.
  10. 10. A planting method for synergistically improving soybean yield and seed quality, the method comprising: Constructing a soybean GmCKX/GmCKX 14 mutant by the method of any one of claims 1 to 9, and Planting the soybean GmCKX/GmCKX 14 mutant.

Description

Method for synergistically improving soybean yield and seed quality Technical Field The application belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to a method for synergistically improving soybean yield and seed quality. Background The soybean is used as a global important grain and oil crop and is one of the main sources of human high-quality vegetable protein and edible oil. Increasing soybean yield and improving quality (especially protein content) have been central targets for breeding efforts. However, there is often a significant negative correlation between soybean yield and quality traits (such as protein and lipid content), i.e., high yield is often accompanied by a decrease in protein content or a decrease in lipid content, which is a long-standing problem in genetic improvement of soybeans. Several approaches for improving a specific property of soybean by regulating individual genes or using molecular marker-assisted selection have been disclosed in the prior art, such as CN118291671a discloses gene GmPGK related to soybean oil content and hundred grain weight, CN110041416a discloses the use of GmABCA gene in increasing soybean protein content and grain weight, CN106701784A discloses the use of oil body protein gene GmOLE01 in increasing soybean oil content. However, these technical schemes focus on genetic improvement of single traits, and cannot realize cooperative improvement of yield and quality traits. At present, the prior art scheme has not seen the report that the negative correlation between soybean yield and quality traits can be effectively broken, the cooperative improvement of the yield and protein content can be realized, and meanwhile, the stable grease content can be maintained. Disclosure of Invention Based on this, an embodiment of the present application provides a method for synergistically improving soybean yield and seed quality. In one aspect, the present application provides a method for synergistically improving soybean yield and seed quality, comprising: Performing gene editing on a target region on a second exon of a target gene of soybean to construct a soybean GmCKX/GmCKX mutant; the target region comprises a target region shown in SEQ ID NO.1 and a target region shown in SEQ ID NO. 2. In some of these embodiments, the soybean GmCKX/GmCKX 14 mutant is subject to deletion mutations and/or insertion mutations in the target region. In some of these embodiments, the soybean GmCKX/GmCKX 14 mutant inserts a single base in the target region shown in SEQ ID NO. 1. In some of these embodiments, the soybean GmCKX/GmCKX 14 mutant lacks multiple bases at the target region shown in SEQ ID No. 2; in some of these embodiments, the soybean GmCKX/GmCKX 14 mutant lacks 3-5 bases in the target region shown in SEQ ID NO. 2. In some of these embodiments, the soybean GmCKX/GmCKX 14 mutant has the fragment shown as SEQ ID NO.5 and SEQ ID NO. 7. In some of these embodiments, gene editing includes employing one or more of CRISPR/Cas9, CRISPR/Cpf1, CRISPR/Cas12i/j, TALEN, ZFN, and back-complementing the relevant target gene knockout; in some of these embodiments, gene editing employs CRISPR/Cas9 technology. In some of these embodiments, the variety of soybeans includes Williams 82. In another aspect, the present application provides a planting method for synergistically improving soybean yield and seed quality, the method comprising: Constructing soybean GmCKX/GmCKX 14 mutant by the method, and planting the soybean GmCKX/GmCKX 14 mutant. The present application provides a method for synergistically improving soybean yield and seed quality, comprising gene editing a target region on a second exon of a target gene of soybean using a gene editing technique, generating frame shift mutations and causing premature termination of protein translation by simultaneous targeted editing of soybean cytokinin oxidase/dehydrogenase genes GmCKX and GmCKX. According to the method, two key cytokinin oxidase genes are knocked out simultaneously, so that the negative correlation between the yield and the protein content in traditional soybean breeding is broken, synchronous improvement of the yield and the seed protein content is realized, and meanwhile, the stability of the oil content is maintained. Wherein, the coordinated knockout of GmCKX and GmCKX14 genes can effectively regulate the steady-state balance of cytokinin, and by influencing the cell division and differentiation process, not only the increase of yield constituent factors is promoted, but also the normal progress of seed protein anabolism is maintained, thereby breaking through the property balance limit of high yield-low protein. Drawings In order to more clearly illustrate the technical solution in the embodiments of the present application and to more fully understand the present application and its advantageous effects, the following brief description will be given with reference