CN-121992013-A - Application of tea tree transcription factor CsMYB26 in regulation and control of anthracnose resistance of plants

Abstract

The invention discloses application of a tea tree transcription factor CsMYB26 in regulating plant anthracnose resistance, firstly discovers and verifies the regulation and control effect of the tea tree transcription factor CsMYB26 in plant anthracnose resistance, clearly determines the biological function of the tea tree transcription factor CsMYB26 as a disease resistance related transcription factor, overcomes the problem of insufficient knowledge of the gene function in the prior art, can be applied to the genetic improvement of anthracnose resistance of tea trees and other plants as an important disease resistance gene resource with common regulation and control effect, provides a preferable candidate gene for molecular breeding of disease resistance varieties, has better stability and sustainability based on a disease resistance strategy in a gene regulation level, is favorable for reducing the use amount of pesticides, and has good ecological benefit and application prospect compared with the traditional technology relying on chemical control.

Inventors

• Lv Wuyun
• WANG YUCHUN
• GUAN SUZHEN
• CHEN JUNJIE
• CHEN YANAN
• Ren Hengze
• LU MENGQIAN

Assignees

• 浙江农林大学

Dates

Publication Date

20260508

Application Date

20251230

Claims (8)

1. 1. An application of a tea tree transcription factor CsMYB26 in regulating and controlling anthracnose resistance of plants.
2. 2. The use according to claim 1, wherein the plants comprise woody plants and herbaceous plants.
3. 3. The use according to claim 2, comprising tea tree and tobacco.
4. 4. The use according to claim 1, wherein the nucleotide sequence of the tea tree transcription factor CsMYB26 gene is shown in SEQ ID No. 1.
5. 5. The use according to claim 1, wherein the use is the overexpression of the tea tree transcription factor CsMYB26 in the plant genome.
6. 6. The use according to claim 5, wherein the over-expression is to link the tea tree transcription factor CsMYB26 with a plant expression vector pCAMBIA2300 or pCAMBIAsuper1300 with a 35S promoter, and transfer the plant into a plant genome by an agrobacterium-mediated method or a leaf disc method to improve the anthracnose resistance of the plant.
7. 7. Use of the tea tree transcription factor CsMYB26 of claim 1 for increasing anthracnose resistance in a plant.
8. 8. The use according to claim 7, wherein the use is the overexpression of the tea tree transcription factor CsMYB26 in plant genes.

Description

Application of tea tree transcription factor CsMYB26 in regulation and control of anthracnose resistance of plants Field of the art The invention belongs to the technical field of biological genetic engineering, and particularly relates to application of a tea tree transcription factor CsMYB26 in regulation and control of anthracnose resistance of plants. (II) background art Tea tree [ CAMELLIA SINENSIS (L.) O.Kuntze ] belongs to a tea plant of Camellia genus of Theaceae family, and is one of the most important leaf commercial crops in China. The leaves of the tea tree can be processed to be made into various types of tea leaves, such as green tea, black tea, oolong tea and the like, and the tea leaves are popular in China and are also sold in various places in the world, so that the tea leaves become important commodities in international trade. Therefore, the quality and safety problems of tea are widely paid attention to. Anthracnose caused by Colletotrichum (Colletotrichum) fungi is one of common diseases in tea gardens, and seriously affects the yield and quality of tea, so that the yield of the tea is reduced, and the economic development of the tea is endangered. Therefore, the breeding and planting of the high disease-resistant tea tree variety can reduce the harm of anthracnose to tea trees, and further improve the quality and yield of tea leaves, and has great significance. MYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor is one of the largest transcription factor families in plants, and is widely involved in the processes of plant growth and development, secondary metabolism, adverse stress response and the like through DNA Binding Domain (DBD) and DNA binding. The number of MYB conserved domains contained in the N end can be divided into 4 types of 1R-MYB/MYB-related, R2R3 MYB, 3R-MYB (R1R 2R 3-MYB) and 4R-MYB, wherein the R2R3-MYB is special for higher plants and plays an important role in regulating plant growth and development, stress response and the like. The DBD domain consists of about 52 amino acid residues, forms a helix-turn-helix structure, and is capable of binding to a DNA specific sequence, thereby regulating gene expression. The tea tree is a perennial woody plant, adopts conventional hybridization, radiation mutagenesis and other breeding methods, and has the characteristics of complex breeding program, long breeding period and low breeding efficiency. CsMYB26 is an important member of the tea R2R3-MYB family, whose known function is mainly focused on flavonoid metabolic regulation. Studies prove that CsMYB26 positively regulates the biosynthesis of catechin and procyanidins by recognizing MYB response elements in CsLAR (leuco-anthocyanidin reductase gene) promoters, but the function of CsMYB26 in regulating plant disease resistance has not been reported. (III) summary of the invention The invention aims to provide an application of a tea tree transcription factor CsMYB26 in regulating and controlling anthracnose resistance of plants, wherein the CsMYB26 transcription factor improves the disease resistance of tea trees by regulating and controlling lignin synthesis pathways, provides gene resources for cultivating high-anthracnose-resistance tea tree varieties, and has excellent potential application value. The technical scheme adopted by the invention is as follows: The invention provides an application of a tea tree transcription factor CsMYB26 in regulating and controlling anthracnose resistance of plants. Further, the plants include woody plants and herbaceous plants including tea tree and tobacco. Further, the nucleotide sequence of the tea tree transcription factor CsMYB26 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. Further, the use is the overexpression of the tea tree transcription factor CsMYB26 in plant genomes, including transient overexpression and stable overexpression. Furthermore, the over-expression is to connect the tea tree transcription factor CsMYB26 with a plant expression vector pCAMBIA2300 or pCAMBIAsuper1300 with a 35S promoter, and transfer the tea tree transcription factor CsMYB26 into a plant genome by adopting an agrobacterium-mediated method or a leaf disc method, so that the anthracnose resistance of the plant is improved. The invention also provides an application of the tea tree transcription factor CsMYB26 in improving anthracnose resistance of plants, wherein the application is that the tea tree transcription factor CsMYB26 is overexpressed in plant genes. The CsMYB26 gene sequences to which the invention relates have been submitted to NCBI (GenBank accession number: PX 609948) and published in the TPIA database (https:// TPIA. Tea plant transgenic technology system is not mature, but Nicotiana benthamiana is a model plant in the field of plant biology research. Therefore, the CsMYB26 gene can be overexpressed in Nicotiana benthamiana through agrobacterium-