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CN-121992015-A - Protein for regulating and controlling cereal crop yield and application thereof

CN121992015ACN 121992015 ACN121992015 ACN 121992015ACN-121992015-A

Abstract

The invention provides a protein for regulating and controlling the yield of cereal crops and application thereof, and discovers that GSN7 is a functional protein involved in regulating and controlling the yield of cereal crops, reduces the expression of the protein or a coding gene thereof, can effectively improve the grain number per spike, the yield per plant and the yield per cell of cereal crops, and has no obvious negative influence on other yield-related characters such as plant height, tillering number, thousand kernel weight and the like. The invention overcomes the common negative correlation among the yield traits, breaks through the technical bottleneck of mutual restriction among a plurality of yield traits, and has important value for driving the progress of high-yield breeding technology and germplasm innovation.

Inventors

  • LIU XUEYING
  • WU YUNZHE
  • TIAN YI
  • WU KUN
  • FU XIANGDONG

Assignees

  • 中国科学院遗传与发育生物学研究所

Dates

Publication Date
20260508
Application Date
20260113

Claims (10)

  1. The application of GSN7 protein and/or GSN7 gene in improving cereal crop yield is characterized in that the amino acid sequence of the GSN7 protein is shown as SEQ ID NO 3 or SEQ ID NO 6, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6 and has the same function.
  2. The application of GSN7 protein and/or GSN7 gene in improving the grain number per ear, single plant yield and/or cell yield of cereal crops is characterized in that the amino acid sequence of the GSN7 protein is shown as SEQ ID NO 3 or SEQ ID NO 6, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6 and has the same function.
  3. 3. The use of biological material for reducing GSN7 protein expression or activity and/or GSN7 gene expression level in improving cereal crop yield is characterized in that the amino acid sequence of GSN7 protein is shown as SEQ ID NO 3 or SEQ ID NO 6, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6 and has the same function.
  4. 4. The use of biological material for reducing GSN7 protein expression or activity and/or GSN7 gene expression level in improving grain number per ear, single plant yield and/or district yield of cereal crops is characterized in that the amino acid sequence of GSN7 protein is shown as SEQ ID NO 3 or SEQ ID NO 6, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6 and has the same function.
  5. 5. The method of claim 1 or 3, wherein the increasing cereal crop yield comprises increasing the number of grains per ear, the individual yield, and/or the cell yield of the cereal crop.
  6. 6. The use of claim 1,3 or 5, wherein the biological material comprises a nucleic acid molecule, an expression cassette, a vector, a cell or a microorganism.
  7. 7. The use according to any one of claims 1 to 6, wherein the nucleotide sequence of the GSN7 gene is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO.5, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the nucleotide sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO.5, and is capable of encoding a protein having the same function.
  8. 8. The use according to any one of claims 1 to 6, wherein the target cereal crop comprises rice.
  9. 9. A method for increasing the yield of a cereal crop comprising the steps of: reducing GSN7 protein expression or activity and/or GSN7 gene expression levels in a cereal crop of interest; The amino acid sequence of the GSN7 protein is shown as SEQ ID NO 3 or SEQ ID NO 6, or Has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6 and has the same function.
  10. 10. The method of claim 9, wherein the target cereal crop comprises rice.

Description

Protein for regulating and controlling cereal crop yield and application thereof Technical Field The invention relates to the technical field of molecular genetic breeding, in particular to a protein for regulating and controlling the yield of cereal crops and application thereof. Background Rice (Oryza sativa l.) is a major food crop, and its yield enhancement is important to ensure food safety and agricultural sustainable development. The yield of the rice is determined by three factors of effective spike number, spike number and thousand grain weight, wherein the spike number is directly related to the yield of the rice, and the spike number is always one of hot spots for high-yield breeding research of the rice. These traits are often typical quantitative traits, controlled by complex Quantitative Trait Loci (QTLs). With the completion of rice genome sequencing and the development of molecular genetics, those skilled in the art have found that a plurality of important QTLs or genes related to rice yield, for example, DEP1, APO1, LAX1, osCKX2, ghd7, RCN1 and RCN2, are involved in regulating the number of spike grains, and the molecular mechanism of rice yield formation is gradually known. However, because the relationships among the yield-related agronomic traits such as effective spike number, thousand grain weight and the like are complicated, and are related to each other and are mutually restricted (for example, spike number and thousand grain weight), breaking the negative correlation among the yield traits and realizing the decoupling of the traits are key research directions of the current technicians in the field. The gene for controlling the grain number of the ears is commonly used for simultaneously controlling a plurality of other yield traits, is 'multi-effect', for example, IPA1 is used for simultaneously controlling the grain number of the ears, the tiller number, the stem thickness and other traits, DEP1 is used for simultaneously controlling the grain number of the ears, the plant height, the grain weight and other traits, so that the negative correlation among the traits is still lacking at present, and the gene can be directly used for key genes of rice high-yield breeding. Disclosure of Invention In response to the above-identified deficiencies or improvements in the prior art, the present invention provides a protein for regulating cereal crop yield and uses thereof to break the negative correlation between yield traits. It is a first object of the present invention to provide the use of GSN7 protein and/or GSN7 gene in increasing the yield of cereal crops. A second object of the present invention is to provide the use of GSN7 protein and/or GSN7 gene in increasing the number of grains per ear, individual yield and/or cell yield of a cereal crop. A third object of the present invention is to provide the use of a biomaterial that reduces GSN7 protein expression or activity and/or GSN7 gene expression level in improving cereal crop yield. A fourth object of the present invention is to provide the use of a biomaterial that reduces GSN7 protein expression or activity and/or GSN7 gene expression level in increasing grain per ear, individual yield and/or cell yield of a cereal crop. It is a fifth object of the present invention to provide a method for increasing the yield of cereal crops. In order to achieve the above purpose, the specific technical scheme of the invention is as follows: The GSN7 protein and/or the application of GSN7 gene in improving cereal crop yield, wherein the amino acid sequence of the GSN7 protein is shown as SEQ ID NO. 3 or SEQ ID NO. 6, or has at least 80%, 85%, 90%, 95%, 98% or 99% identity with the amino acid sequence shown as SEQ ID NO. 3 or SEQ ID NO. 6, and has the same function. As a preferred embodiment, the increasing the yield of the cereal crop comprises increasing the number of grains per ear, the yield per plant and/or the yield per cell of the cereal crop. As a preferred embodiment, GSN7 protein expression or activity and/or GSN7 gene expression levels in said cereal crop are reduced. As a preferred embodiment, the GSN7 gene has a nucleotide sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO.5, or has at least 80%, 85%, 90%, 95%, 98% or 99% identity with a nucleotide sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO.5, and is capable of encoding a protein having the same function. As a preferred embodiment, the biological material is selected from the group consisting of a nucleic acid molecule, an expression cassette, a recombinant vector, a genetically engineered host cell or a microorganism. As a preferred embodiment, the cereal crop is rice. As a more preferable scheme, the rice is indica rice, or japonica rice, or the filial generation of japonica rice and indica rice. The use of GSN7 protein and/or GSN7 gene in improving grain number per ear, single plant yield and/or district yield of cereal crops, wh