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CN-121992020-A - Low-temperature-resistant related protein IbbHLH-129 of sweet potato, related biological material and application thereof

CN121992020ACN 121992020 ACN121992020 ACN 121992020ACN-121992020-A

Abstract

The application discloses a sweet potato low temperature resistant related protein IbbHLH, a related biological material and application thereof, and relates to the field of genetic engineering. In order to improve the cold tolerance of sweet potatoes, the application provides IbbHLH protein and the application of the coding gene thereof in regulating the cold tolerance of the sweet potatoes, and sweet potato plants transformed with IbbHLH gene are obtained by regulating the content or the expression of the IbbHLH protein in the sweet potatoes. Compared with wild sweet potato plants, the sweet potato plants over-express IbbHLH gene have reduced wilting degree under cold treatment, which shows that IbbHLH protein and encoding gene play an important role in regulating and controlling cold tolerance of plants. The IbbHLH protein and the coding gene thereof provided by the application have important application value in regulating and controlling the cold resistance of plants, and have wide application space and market prospect in the agricultural field.

Inventors

  • YU TAO
  • LIU ZHENLEI
  • YANG ZITONG
  • Pan Jiachuo
  • LIU SITONG

Assignees

  • 辽宁省农业科学院

Dates

Publication Date
20260508
Application Date
20260212

Claims (10)

  1. Use of an IbbHLH129 protein or a substance that modulates the expression of a gene encoding the IbbHLH protein or a substance that modulates the activity or content of the IbbHLH protein in any one of, A1 Application in regulating plant cold tolerance; A2 Application in preparing products for regulating and controlling plant cold resistance; A3 Application in plant cold tolerance breeding or auxiliary breeding; a4 Application in the preparation of plant cold tolerance breeding or auxiliary breeding products; the IbbHLH protein 129 is any one of the following proteins: a1 A protein with an amino acid sequence shown as SEQ ID NO. 1; a2 A protein obtained by substituting and/or deleting and/or adding the amino acid residues in the amino acid sequence shown in a 1); a3 A protein having at least 70% identity with the amino acid sequence shown in a 1) and having the same function; a4 A fusion protein obtained by ligating a tag to the N-terminus or/and the C-terminus of at least one of the proteins of a 1) to a 3).
  2. 2. The use according to claim 1, wherein the substance regulating the expression of the gene encoding IbbHLH protein 129 or the substance regulating the activity or content of IbbHLH protein is a biological material, which is any of the following: b1 A nucleic acid molecule encoding the IbbHLH129,129 protein of claim 1; B2 An expression cassette comprising the nucleic acid molecule of B1); B3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell comprising B1) said nucleic acid molecule or a transgenic plant cell comprising B2) said expression cassette or a transgenic plant cell comprising B3) said recombinant vector; B6 A transgenic plant tissue comprising B1) said nucleic acid molecule or a transgenic plant tissue comprising B2) said expression cassette or a transgenic plant tissue comprising B3) said recombinant vector; B7 A transgenic plant organ comprising the nucleic acid molecule of B1) or a transgenic plant organ comprising the expression cassette of B2) or a transgenic plant organ comprising the recombinant vector of B3).
  3. 3. The use according to claim 2, wherein the nucleic acid molecule of B1) is a DNA molecule according to g 1) or g 2) as follows: g1 A DNA molecule with the coding sequence of the coding strand being SEQ ID NO. 2; g2 A DNA molecule having more than 70% identity to the DNA molecule of g 1) and encoding the IbbHLH protein of claim 1.
  4. 4. A method of modulating cold tolerance in a plant, the method comprising modulating the expression of a gene encoding the IbbHLH129,129 protein of claim 1 in a recipient plant and/or modulating the activity or content of the IbbHLH129,129 protein in the recipient plant to modulate cold tolerance in the recipient plant.
  5. 5. A method for obtaining a plant of interest with altered cold tolerance, comprising obtaining a plant of interest with altered cold tolerance by modulating the expression level of a gene encoding the IbbHLH protein of claim 1 in a recipient plant and/or modulating the activity or content of the IbbHLH protein in a recipient plant.
  6. 6. The method according to claim 4 or 5, characterized in that the method comprises increasing the cold tolerance of the recipient plant or/and obtaining a plant of interest with increased cold tolerance by increasing the expression of the gene encoding the IbbHLH protein of claim 1 in the recipient plant and/or modulating the activity or content of the IbbHLH protein in the recipient plant.
  7. 7. The method of claim 6, wherein increasing the expression level of the gene encoding the IbbHLH129 protein in the recipient plant and/or increasing the activity or content of the IbbHLH protein in the recipient plant is achieved by introducing into the recipient plant a gene encoding the IbbHLH protein of claim 1.
  8. 8. A cold tolerant plant, which is a plant comprising the biological material of claim 2 or 3.
  9. 9. The method according to any one of claims 1 to 8, wherein the plant is a dicotyledonous plant.
  10. 10. The IbbHLH protein of claim 1 and the biomaterial of claim 2 or 3.

Description

Low-temperature-resistant related protein IbbHLH-129 of sweet potato, related biological material and application thereof Technical Field The application relates to the field of genetic engineering, in particular to a sweet potato low temperature resistant related protein IbbHLH and a related biological material and application thereof. Background Low temperatures can severely inhibit plant growth. It reduces cellular enzymatic activity, slows photosynthesis and respiration, and results in a hindered synthesis and absorption of nutrients. If the temperature suddenly drops, the cell gap is easy to freeze, the cell membrane structure is destroyed, and the cell dehydration necrosis is induced. For crops, low temperature can delay germination and frostbite seedlings, and abnormal flowering and pollination can be caused, so that flower and fruit dropping can occur. In addition, low temperature weakens stress resistance of plants, so that the plants are more susceptible to attack by diseases and insect pests, and finally, crop yield reduction, quality reduction and even whole plant death are caused. Sweet potatoes (Ipomoea batatas (L.) lam.) are important food economic crops and novel energy crops widely planted worldwide, and have great significance for guaranteeing food safety. Sweet potato is good at Wen Wei cold, is easy to cause frostbite of seedling and swelling and stagnation of root tuber, and decays and reduces yield when serious. Spring cold and autumn frost frequently occur in multiple areas of China, and the planting range and yield of sweet potatoes are restricted. Developing the research of sweet potato cold resistance, cultivating cold-resistant varieties, improving the stress resistance of sweet potato, guaranteeing the growth and yield of sweet potato in low-temperature environment, and having important practical significance for stabilizing the sweet potato production and widening the planting area. However, the mechanism of cold-tolerant transcriptional regulation of sweet potato is not clear. How to excavate the related genes involved in cold resistance regulation, the cold resistance mechanism is studied deeply, and the regulation and control of plant cold resistance are vital through plant genetic engineering. Disclosure of Invention The application aims to solve the technical problem of how to regulate and control the cold resistance of sweet potatoes. More specifically, the application aims to solve the technical problem of improving the cold resistance of sweet potatoes. In order to solve the technical problem, the application provides the following technical scheme: The application provides the use of IbbHLH protein or a substance which regulates the expression of the IbbHLH protein-encoding gene or a substance which regulates the activity or content of the IbbHLH protein in any one of the following, A1 Application in regulating plant cold tolerance; A2 Application in preparing products for regulating and controlling plant cold resistance; A3 Application in plant cold tolerance breeding or auxiliary breeding; a4 Application in the preparation of plant cold tolerance breeding or auxiliary breeding products; the IbbHLH protein 129 is any one of the following proteins: a1 A protein with an amino acid sequence shown as SEQ ID NO. 1; a2 A protein obtained by substituting and/or deleting and/or adding the amino acid residues in the amino acid sequence shown in a 1); a3 A protein having at least 70% identity with the amino acid sequence shown in a 1) and having the same function; a4 A fusion protein obtained by ligating a tag to the N-terminus or/and the C-terminus of at least one of the proteins of a 1) to a 3). In the present application, the modulation may be an increase or promotion or up-regulation. In the present application, the modulation may also be reduction or inhibition or downregulation. In the present application, the plant breeding index includes cold tolerance. In the present application, the object of plant breeding includes growing plants with altered cold tolerance. In particular, the object of plant breeding includes growing plants with increased cold tolerance. In the present application, the protein may be derived from sweet potato. The protein can be synthesized artificially or obtained by synthesizing the coding gene and then biologically expressing. In the above protein, the linkage may be such that adjacent amino acids (or amino acid residues) are linked by peptide bonds. The protein tag (protein-tag) refers to a polypeptide or protein which is fused and expressed together with a target protein by using a DNA in-vitro recombination technology so as to facilitate the expression, detection, tracing and/or purification of the target protein. The protein tag may be a Flag protein tag, a His protein tag, an MBP protein tag, an HA protein tag, a myc protein tag, a GST protein tag, and/or a SUMO protein tag, etc. In the application, the substance for regulating and controllin