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CN-121992021-A - Low-temperature-resistant related protein IbNAC and related biological material and application thereof

CN121992021ACN 121992021 ACN121992021 ACN 121992021ACN-121992021-A

Abstract

The application discloses a sweet potato low temperature resistant related protein IbNAC and a related biological material and application thereof, belonging to the technical field of genetic engineering. The application provides IbNAC protein and application of a coding gene thereof in regulation and control of cold tolerance of sweet potatoes, and sweet potato plants transformed with IbNAC genes are obtained by regulating and controlling the content or expression of IbNAC protein in sweet potatoes, and experiments show that compared with wild sweet potato plants, the sweet potato plants over-expressed with IbNAC genes have reduced wilting degree under cold treatment, and the IbNAC protein and the coding gene play an important role in regulating and controlling the cold tolerance of plants. The IbNAC protein and the coding gene thereof provided by the application have important application value in regulating and controlling the cold resistance of plants, and have certain application space and market prospect in the agricultural field.

Inventors

  • YU TAO
  • LIU ZHENLEI
  • YANG ZITONG
  • Pan Jiachuo
  • LIU SITONG

Assignees

  • 辽宁省农业科学院

Dates

Publication Date
20260508
Application Date
20260212

Claims (10)

  1. 1. A cold-tolerant plant comprises an exogenous gene encoding IbNAC90,90 protein, The IbNAC protein is any one of the following proteins: a1 A protein with an amino acid sequence shown as SEQ ID NO. 1; a2 A protein obtained by substituting and/or deleting and/or adding the amino acid residues in the amino acid sequence shown in a 1); a3 A protein having at least 70% identity with the amino acid sequence shown in a 1) and having the same function; a4 A fusion protein obtained by ligating a tag to the N-terminus or/and the C-terminus of at least one of the proteins of a 1) to a 3).
  2. 2. The use of a IbNAC protein as claimed in claim 1 or a substance regulating the expression of a gene encoding said IbNAC protein or a substance regulating the content of said IbNAC protein in any one of, A1 Application in regulating plant cold tolerance; A2 Application in preparing products for regulating and controlling plant cold resistance; A3 Application in plant cold tolerance breeding or auxiliary breeding; A4 For the production of plant cold tolerance breeding or for the production of auxiliary breeding.
  3. 3. The use according to claim 2, wherein the substance regulating the expression of the gene encoding the IbNAC90 protein or the substance regulating the content of the IbNAC protein is a biological material, the biological material being any of the following: b1 A nucleic acid molecule encoding the IbNAC90,90 protein of claim 1; B2 An expression cassette comprising the nucleic acid molecule of B1); B3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), a recombinant microorganism comprising the expression cassette of B2) or a recombinant microorganism comprising the recombinant vector of B3); B5 A transgenic plant cell line comprising B1) said nucleic acid molecule, a transgenic plant cell line comprising B2) said expression cassette or a transgenic plant cell line comprising B3) said recombinant vector; B6 A transgenic plant tissue comprising B1) said nucleic acid molecule, a transgenic plant tissue comprising B2) said expression cassette or a transgenic plant tissue comprising B3) said recombinant vector; B7 A transgenic plant organ comprising the nucleic acid molecule of B1), a transgenic plant organ comprising the expression cassette of B2) or a transgenic plant organ comprising the recombinant vector of B3).
  4. 4. Use according to claim 3, characterized in that the nucleic acid molecule of B1) is a DNA molecule according to g 1) or g 2) as follows: g1 A DNA molecule with the coding sequence of the coding strand being SEQ ID NO. 2; g2 A DNA molecule having more than 70% identity to the DNA molecule of g 1) and encoding the IbNAC protein of claim 1.
  5. 5. A method for regulating cold tolerance in a plant, comprising the step of regulating the expression of a gene encoding the IbNAC90 protein of claim 1 in a recipient plant and/or regulating the amount of the IBNAC90 protein in the recipient plant to regulate cold tolerance in the recipient plant.
  6. 6. A method for obtaining a plant of interest with altered cold tolerance, comprising the step of modulating the expression level of a gene encoding the IbNAC90 protein of claim 1 in a recipient plant and/or modulating the content of the IbNAC90 protein in a recipient plant to obtain a plant of interest with altered cold tolerance.
  7. 7. The method according to claim 5 or 6, characterized in that it comprises a step of increasing the cold tolerance of the recipient plant or/and obtaining a plant of interest with increased cold tolerance by increasing the expression level of the gene encoding the IbNAC90 protein of claim 1 in the recipient plant and/or regulating the content of the IbNAC protein in the recipient plant.
  8. 8. The method of claim 7, wherein said increasing the expression level of said IbNAC protein encoding gene in a recipient plant and/or increasing the amount of said IbNAC protein in a recipient plant is achieved by introducing into a recipient plant a gene encoding said IbNAC protein of claim 1.
  9. 9. The method according to any one of claims 5 to 8, wherein the IbNAC protein-encoding gene is the DNA molecule according to g 1) or g 2) as follows: g1 A DNA molecule with the coding sequence of the coding strand being SEQ ID NO. 2; g2 A DNA molecule having more than 70% identity to the DNA molecule of g 1) and encoding the IbNAC protein of claim 1.
  10. 10. The IbNAC protein of claim 1 and the biomaterial of claim 3 or 4.

Description

Low-temperature-resistant related protein IbNAC and related biological material and application thereof Technical Field The application belongs to the technical field of genetic engineering, and particularly relates to a sweet potato low-temperature-resistant related protein IbNAC and a related biological material and application thereof. Background Low temperature acts as a global abiotic stress factor, and constitutes a systemic threat to plant survival and development. The low temperature causes a sudden drop in photosynthetic rate, resulting in energy metabolism disorders and massive accumulation of Reactive Oxygen Species (ROS), disrupting the integrity and permeability of the membrane system, disrupting organelle structure, and ultimately leading to apoptosis. For crop production, low-temperature stress runs through the whole growth period, so that not only can seed germination rate be reduced and seedlings become yellow and withered, but also reproductive growth process can be disturbed, flowering period delay and pollen activity reduction are caused, fruiting rate reduction and sharp yield reduction are finally caused, and even continuous crop harvest is caused seriously. Sweet potatoes (l.) lam.) are important crops originating in tropical and subtropical regions, and have multiple values of food safety guarantee, economic income increase and energy development. The tuberous root is rich in starch, dietary fiber and various functional components, and is not only a staple food source of billions of people worldwide, but also a core raw material for starch processing and biofuel production. Sweet potato naturally has typical temperature preference, the optimum growth and development temperature is 22-30 ℃, when the ambient temperature is lower than 15 ℃, the root absorption capacity is obviously reduced, the tuberous root is expanded and basically stagnates, when the ambient temperature is lower than 10 ℃, the photosynthesis function of the leaf is lost, the breathing of tuberous root cells is abnormal, saccharification and browning are easy to occur, if frost or low temperature below 0 ℃, the overground parts of plants rapidly wilt and die, and the underground tuberous root is decomposed due to cell icing, so that destructive loss is caused. The spring sowing period in northern areas of China often encounters 'reverse spring cold', autumn in southern areas of China is subjected to early frost invasion, and the low-temperature disasters severely restrict the expansion of the planting north boundary and annual production layout of sweet potatoes, so that the method becomes a core bottleneck for limiting the high-quality development of the sweet potato industry. At present, the molecular regulation network of sweet potatoes responding to low temperature stress is not perfect. It is important to dig key cold-resistant gene resources, analyze the molecular mechanism of plant cold resistance and directionally improve the cold resistance of plants by means of genetic engineering. Disclosure of Invention The application aims to solve the technical problem of how to regulate and control the cold resistance of sweet potatoes. More specifically, the application aims to solve the technical problem of improving the cold resistance of sweet potatoes. In order to solve the technical problem, the application provides the following technical scheme: The present application provides a cold tolerant plant comprising an exogenous gene encoding a IbNAC protein, The IbNAC protein is any one of the following proteins: a1 A protein with an amino acid sequence shown as SEQ ID NO. 1; a2 A protein obtained by substituting and/or deleting and/or adding the amino acid residues in the amino acid sequence shown in a 1); a3 A protein having at least 70% identity with the amino acid sequence shown in a 1) and having the same function; a4 A fusion protein obtained by ligating a tag to the N-terminus or/and the C-terminus of at least one of the proteins of a 1) to a 3). In the present application, the exogenous gene encoding IbNAC.sup.90 protein is the DNA molecule described in g 1) or g 2) as follows: g1 A DNA molecule with the coding sequence of the coding strand being SEQ ID NO. 2; g2 A DNA molecule which has 70% or more identity with the DNA molecule of g 1) and encodes the IbNAC protein. In one embodiment of the application, the nucleotide sequence of the exogenous gene is SEQ ID NO. 2. In the present application, the protein may be derived from sweet potato. The protein can be synthesized artificially or obtained by synthesizing the coding gene and then biologically expressing. In the above protein, the linkage may be such that adjacent amino acids (or amino acid residues) are linked by peptide bonds. The protein tag (protein-tag) refers to a polypeptide or protein which is fused and expressed together with a target protein by using a DNA in-vitro recombination technology so as to facilitate the expression, detection, tracing a