CN-121992023-A - Application of HDA15 gene in regulating and controlling leaf apex angle of plant leaf

Abstract

The invention provides an application of an HDA15 gene in regulating and controlling the leaf apex angle of a plant leaf. According to the invention, an antisense inhibition gene silencing plant of the tea tree HDA15 gene is successfully constructed for the first time, and the leaf apex exhibition angle is measured, so that the tea tree HDA15 gene is found to play a positive promotion role in leaf development, and the antisense inhibition of the HDA15 gene can reverse the role, namely the leaf apex exhibition angle of the tea tree is reduced, and a new way is opened for efficient mechanical exploitation of tea leaves.

Inventors

• ZHAI XIUMING
• LIU HAORAN
• LI JIE
• XIAO FULIANG
• DUAN LINGXIAO
• TANG YI

Assignees

• 重庆市农业科学院

Dates

Publication Date

20260508

Application Date

20260225

Claims (10)

1. The application of HDA15 gene in regulating and controlling the leaf apex angle of plant leaves.
2. 2. The use according to claim 1, wherein the plant is tea tree.
3. 3. The use according to claim 2, wherein the nucleotide sequence of the HDA15 gene is shown in SEQ ID No. 1.
4. 4. Use according to any one of claims 1-3, wherein the modulation of the tip spread of a plant leaf is a reduction of the tip spread.
5. 5. The use according to claim 4, wherein the apex spread is reduced by gene interference or gene silencing.
6. 6. The use according to claim 5, wherein the gene interference or gene silencing is achieved by antisense primers.
7. 7. The use according to claim 6, wherein the antisense primer is selected from the group consisting of one or more of the sequences set forth in SEQ ID NOS.4-8.
8. 8. An antisense inhibition treatment liquid for HDA15 gene is prepared by mixing five sequences shown in SEQ ID NO. 4-8 in equal quantity, and adding distilled water for dilution, wherein the concentration of each primer in the antisense inhibition treatment liquid is 20+/-2 mu M.
9. 9. A kit for silencing an HDA15 gene comprising the antisense suppression treatment fluid of claim 8.
10. 10. A method of reducing leaf tip angle spread of tea tree leaves, comprising treating the growing tea tree shoots with the antisense suppression treatment fluid of claim 8 or the kit of claim 9.

Description

Application of HDA15 gene in regulating and controlling leaf apex angle of plant leaf Technical Field The invention belongs to the technical field of biology, and particularly relates to application of an HDA15 gene in regulating and controlling leaf tip angle of a plant leaf, in particular to application in regulating and controlling leaf tip angle of a tea tree. Background Tea is one of the three important non-alcoholic beverages worldwide. The tea tree in China has rich germplasm resources, high yield value and large scale. In the past, tea harvesting has relied substantially on manual harvesting. Mechanical harvesting (mechanical harvesting) is now becoming a growing concern in the tea industry due to reduced labor force caused by aging population. Tea trees with suitable mechanical properties are important in the development of the tea industry. The leaf tip angle is a main factor affecting the integrity rate of mechanically picked bud leaves. Therefore, how to regulate and control the leaf tip angle of the tea tree becomes the focus of the breeding of the mechanically picked tea tree. The blade tip spread angle is the connecting line angle between the blade tip and the blade landing point, and the currently commonly used blade included angle in the industry is defined as the connecting line angle between the middle part of the blade vein and the blade landing point. The concept of the leaf angle is not clear enough because the definition standard of the point in the middle of the veins is more fuzzy. Current methods for determining the included angle of tea leaves include image analysis or direct measurement (for accurate determination) and morphological classification (for rapid determination). The image analysis method is to obtain an image by using a scanner or a digital camera and then analyze the image by using image analysis software (such as Photoshop, imageJ and the like), the direct measurement method uses a protractor, a vernier caliper, a ruler and the like to directly measure angles, the morphological classification method is to directly classify according to the angle of a leaf included angle observed by naked eyes, the upright type leaf is obviously upturned, the included angle between the upright type leaf and a branch is small, the leaf is almost stuck to the branch, the semi-upright type leaf and the branch are in an inclined state and are between the upright type leaf and the branch, the leaf of the branch is flat or sagged outwards, and the included angle between the leaf of the branch is large. However, these methods can only passively determine the leaf angle, and cannot directly perform active regulation to obtain the desired leaf angle. For example, chinese patent application CN116029969a discloses a method for intelligently measuring the included angle of tea buds and leaves and a measuring tool thereof, the measuring method comprises the steps of S1, obtaining a sample picture, S2, intercepting a complete image of one tea bud and one leaf (leaf tip of tea bud is upward and leaf stem is downward), performing binarization treatment on the background, S3, extracting a tea bud skeleton map after edge smoothing treatment and cavity filling by adopting a skeleton extraction algorithm in a computer vision OpenCV library, S4, traversing all pixel points of the skeleton map, obtaining end points and cross point coordinates by adopting an eight-neighborhood discrimination algorithm, and obtaining the end point included angle and cross point included angle by calculating the end points and cross point coordinates. The measuring tool comprises a moving track, and a tea tray is installed in the moving track in a rolling mode. From the research of regulation at the gene level, genes which are known to be involved in regulating plant leaf angles at present include sage LRR receptor-like kinase gene DPY1, brassinosteroid synthase gene and the like. For example, the previous research results of one of the present inventors' units are disclosed in chinese patent application CN116103305A, which discloses the use of the EXO gene of tea tree in regulating leaf morphology leaf angle and leaf sagging. The nucleotide sequence of the EXO gene is shown in SEQ ID NO. 1 disclosed therein, and the EXO gene belongs to one of the brassinosteroid (Brassinosteroids, BR) genes responding to the plant hormone. The tea leaves obtained by EXO gene silencing have smaller leaf angles and lower leaf sagging degree. The application also provides an antisense inhibition treatment fluid for the EXO gene. The included angle between leaves in the study object refers to the included angle value between the stems and the leaves of tea trees (namely the included angle concept of leaves commonly used in the industry at present), and the sagging curvature of the leaves refers to the ratio of the distance between the far and the near ends of the leaves and the length of veins in the leaves in space. Because the definition stan