CN-121992027-A - Application of LecRK gene in regulation and control of drought resistance of tomatoes

Abstract

The invention discloses an application of LecRK gene in regulating drought resistance of tomato, relates to the technical field of plant genetic engineering, and firstly discovers and confirms important role of LecRK gene in regulating drought resistance of tomato, fills up the blank of tomato LecRK gene in drought resistance function research, enriches research content of drought resistance molecular mechanism of tomato, and provides a new view for functional diversity research of plant LecRK gene family. The LecRK gene provided by the invention can obviously improve the drought resistance of tomatoes, can obviously improve the seed germination rate and survival rate of tomatoes under drought stress by expressing the gene, obviously reduce the leaf pore number, the water loss rate and the malondialdehyde content, enhance the adaptability of tomatoes to drought environment, radically improve the drought resistance of tomatoes and solve the problem of poor drought resistance of traditional tomato varieties.

Inventors

• CUI GUIBIN
• QIU LIZHEN
• QU XIAO
• SHEN XIAOLING
• CHEN YIFAN
• TANG NING
• CHEN ZEXIONG

Assignees

• 重庆文理学院

Dates

Publication Date

20260508

Application Date

20260324

Claims (7)

1. The application of the LecRK26 gene in regulating drought resistance of tomatoes is characterized in that the LecRK gene is derived from tomatoes, the nucleotide sequence of the LecRK gene is shown as SEQ ID NO.1 and the nucleotide sequence of the LecRK gene is shown as SEQ ID NO. 2, and the regulation of the drought resistance of tomatoes is specifically to improve the drought resistance of tomatoes, namely, the survival ability and stress resistance of tomatoes under drought stress can be obviously improved by enhancing the expression of LecRK gene.
2. 2. The use of LecRK gene according to claim 1 for controlling drought resistance of tomato, wherein said use is to transfer LecRK gene into tomato plant, over-express LecRK gene, and transfer LecRK gene into tomato plant by one of agrobacterium infection method, gene gun method or polyethylene glycol method.
3. 3. The use of LecRK gene according to claim 1 for regulating drought resistance of tomato, wherein said use is in the cultivation of transgenic tomato varieties with enhanced drought resistance.
4. 4. The recombinant expression vector for regulating drought resistance of tomatoes is characterized in that the nucleotide sequence of the recombinant expression vector is shown as SEQ ID NO. 3.
5. 5. A transgenic cell line or engineering bacterium comprising the recombinant expression vector of claim 4.
6. 6. A method for improving drought resistance of tomatoes is characterized by comprising the following steps of 1) constructing a recombinant expression vector containing LecRK genes in claim 1, 2) transferring the recombinant expression vector into tomato receptor cells to obtain transgenic tomato cells, 3) culturing the transgenic tomato cells into complete plants, and screening out transgenic tomato plants with over-expressed LecRK genes and enhanced drought resistance.
7. 7. The method of claim 6, wherein the drought resistance enhancement in step 3) is characterized by an increase in seed germination rate of transgenic tomatoes under drought stress of 25% -40%, a relative germination rate of 20% -35%, a decrease in leaf stomata number of 15% -28% and a decrease in leaf water loss rate of 20% -35% as compared to wild tomatoes.

Description

Application of LecRK gene in regulation and control of drought resistance of tomatoes Technical Field The invention relates to the technical field of plant genetic engineering, in particular to application of LecRK gene in regulation and control of drought resistance of tomatoes. Background Tomato (Solanum lycopersicum L.) is an important commercial crop widely planted worldwide, has both nutritional and economic values, and occupies an important place in the fields of vegetable production and food processing. However, tomato root system is shallow, transpiration effect is strong, and tomato root system is sensitive to water stress, and drought becomes one of main abiotic stress factors which restrict tomato yield and quality. With the aggravation of global climate change, extreme drought weather is frequent, and the influence of drought stress on tomato production is increasingly prominent, so that tomato plants grow hindered, flower and fruit fall and fruit deformity are caused, and even the plants die when serious, so that huge economic loss is caused. At present, the drought resistance improvement of tomatoes mainly depends on the traditional breeding method and agricultural cultivation measures. The traditional breeding method cultures drought-resistant varieties by screening natural drought-resistant germplasm resources, crossbreeding and other modes, but has the defects of long breeding period, complex genetic background, low drought-resistant character improvement efficiency and the like, and is difficult to meet the urgent demands of modern agricultural production on the drought-resistant varieties of tomatoes. Although agricultural cultivation measures (such as drip irrigation, soil moisture coverage and the like) can relieve drought stress to a certain extent, the problems of high cost, limitation by regional conditions and the like exist, and the drought resistance of tomatoes cannot be fundamentally improved. Along with the rapid development of genetic engineering technology, drought-resistant related genes are excavated by utilizing molecular biological means, drought-resistant crop varieties are cultivated by a transgenic technology, and the method becomes an effective way for improving drought resistance of crops and has the advantages of accuracy, high efficiency, short period and the like. Lectin receptor-like protein kinases (Lectinreceptor-likekinases, lecRKs) are a specific type of transmembrane protein kinase of plants and are widely involved in the processes of plant growth, immune defense, abiotic stress response and the like. Studies have shown that part LecRK genes play an important role in stress responses of drought resistance, salt tolerance and the like of plants, but functional researches on tomato LecRK26 genes are mainly focused on gray mold resistance regulation, and the role and application of the genes in the regulation of drought resistance of tomatoes have not been reported yet. Therefore, lecRK genes related to drought resistance in tomatoes are excavated, the functions and the application methods of the LecRK genes are clear, and the method has important theoretical significance and practical value for enriching the research of drought resistance molecular mechanisms of tomatoes, developing new drought resistance gene resources, cultivating drought resistance tomato varieties and guaranteeing sustainable development of tomato industry. Disclosure of Invention Aiming at the defects in the prior art, the invention provides the application of LecRK gene in regulating and controlling the drought resistance of tomatoes, and the gene is clear to obviously improve the drought resistance of tomatoes, so that a novel gene resource and a novel technical scheme are provided for molecular breeding of drought-resistant varieties of tomatoes. In order to achieve the aim of the invention, the invention adopts the following technical scheme: The LecRK gene is derived from tomato, the nucleotide sequence of the LecRK gene is shown as SEQ ID NO.1, and the nucleotide sequence of the LecRK gene is shown as SEQ ID NO.2, and the regulation and control of the drought resistance of the tomato is specifically to improve the drought resistance of the tomato, namely the viability and stress resistance of the tomato under drought stress can be obviously improved by enhancing the expression of the LecRK gene. Further, the method is characterized in that LecRK genes are transferred into tomato plants, lecRK genes are subjected to over-expression, and the method for transferring LecRK genes into tomato plants is one of an agrobacterium infection method, a gene gun method or a polyethylene glycol method. Further, the method is applied to cultivation of transgenic tomato varieties with enhanced drought resistance. The invention also provides a recombinant expression vector for regulating drought resistance of tomatoes, and the nucleotide sequence of the recombinant expression vector is shown as SEQ I