CN-121992028-A - Application of LecRK gene in regulation and control of salt and alkali tolerance of tomatoes

CN121992028ACN 121992028 ACN121992028 ACN 121992028ACN-121992028-A

Abstract

The invention discloses an application of LecRK gene in regulating and controlling the salt and alkali tolerance of tomatoes, relates to the technical field of plant genetic engineering, and discovers and proves the important role of LecRK gene in regulating and controlling the salt and alkali tolerance of tomatoes for the first time, fills up the blank of the tomato LecRK gene in drought resistance function research, enriches the research content of drought resistance molecular mechanism of tomatoes, and provides a new view for functional diversity research of LecRK gene families of plants. The LecRK gene provided by the invention can obviously improve the salt and alkali resistance of tomatoes, can obviously improve the seed germination rate and survival rate of tomatoes under drought stress by over-expressing the gene, obviously reduce the leaf pore number, the water loss rate and the malondialdehyde content, strengthen the adaptability of tomatoes to saline and alkali environment, radically improve the salt and alkali resistance of tomatoes and solve the problem of poor salt and alkali resistance of traditional tomato varieties.

Inventors

CUI GUIBIN
SHEN XIAOLING
HUANG BAOWEN
QU XIAO
QIU LIZHEN
CHEN YIFAN
ZHU WEI
TANG NING
CHEN ZEXIONG

Assignees

重庆文理学院
重庆大学

Dates

Publication Date: 20260508
Application Date: 20260324

Claims (7)

The application of the LecRK26 gene in regulating and controlling the salt and alkali tolerance of tomatoes is characterized in that the LecRK gene is derived from tomatoes, the nucleotide sequence of the LecRK gene is shown as SEQ ID NO.1 and the nucleotide sequence of the LecRK gene is shown as the nucleic acid molecule of an amino acid sequence shown as SEQ ID NO. 2, and the regulation and control of the salt and alkali tolerance of tomatoes is particularly realized by improving the salt and alkali tolerance of tomatoes, namely, the survivability and stress resistance of tomatoes under drought stress can be obviously improved by enhancing the expression of LecRK gene.
2. The use of LecRK gene according to claim 1 for regulating the saline-alkali tolerance of tomato, wherein the use is to transfer LecRK gene into tomato plant, over-express LecRK gene, and transfer LecRK gene into tomato plant by one of agrobacterium infection method, gene gun method or polyethylene glycol method.
3. Use of LecRK gene according to claim 1 for regulating the saline-alkali tolerance of tomato, wherein said use is in the cultivation of transgenic tomato varieties with enhanced saline-alkali tolerance.
4. A recombinant expression vector for regulating and controlling the saline-alkali tolerance of tomatoes is characterized in that the nucleotide sequence of the recombinant expression vector is shown as SEQ ID NO. 3.
5. A transgenic cell line or engineering bacterium comprising the recombinant expression vector of claim 4.
6. A method for improving the saline-alkali tolerance of tomatoes is characterized by comprising the following steps of 1) constructing a recombinant expression vector containing LecRK genes in claim 1, 2) transferring the recombinant expression vector into tomato receptor cells to obtain transgenic tomato cells, 3) culturing the transgenic tomato cells into complete plants, and screening out transgenic tomato plants with over-expressed LecRK genes and enhanced saline-alkali tolerance.
7. The method of claim 6, wherein the enhancement of the saline-alkali tolerance in the step 3) is characterized in that compared with wild tomatoes, the seed germination rate of the transgenic tomatoes under drought stress is improved by 25% -40%, the relative germination rate is improved by 20% -35%, the number of leaf stomata is reduced by 15% -28%, and the leaf water loss rate is reduced by 20% -35%.

Description

Application of LecRK gene in regulation and control of salt and alkali tolerance of tomatoes Technical Field The invention relates to the technical field of plant genetic engineering, in particular to application of LecRK gene in regulating and controlling salt and alkali tolerance of tomatoes. Background Tomato (Solanum lycopersicum l.) is an important cash crop grown widely worldwide, but saline-alkali stress is one of the main abiotic stresses that restricts its yield and quality. About 20% of cultivated land and 50% of irrigated land worldwide are affected by salinization, and with climate change and irrational irrigation, the salinization area is still continuously expanding. Tomatoes are sensitive to saline-alkali stress, and saline-alkali environment can cause water absorption resistance, ion imbalance and oxidation damage aggravation of root systems of tomatoes, and finally cause growth resistance, yield reduction and even death of plants. At present, the saline-alkali tolerance improvement of tomatoes mainly depends on traditional breeding and cultivation measures, but the traditional breeding period is long, the efficiency is low, the cultivation measures (such as salt washing and modifier application) are high in cost, and the saline-alkali tolerance of tomatoes is difficult to fundamentally improve. The genetic engineering technology provides an efficient way for improving the saline-alkali tolerance of crops, and the excavation of key saline-alkali tolerance genes is a core premise. Lectin receptor-like protein kinase (LecRKs) is a plant-specific transmembrane protein kinase, and has been proved to be involved in the stress response process of plants, but the functions and applications of tomato LecRK26 gene in saline-alkali tolerance regulation have not been reported. Therefore, the effect of LecRK gene in the salt and alkali tolerance of tomatoes is clarified, the application of the gene in molecular breeding is developed, and the gene has important theoretical and practical values for cultivating salt and alkali tolerance tomato varieties and guaranteeing the tomato production in salt and alkali areas. Disclosure of Invention Aiming at the defects in the prior art, the invention provides the application of LecRK gene in regulating and controlling the salt and alkali tolerance of tomatoes, which confirms that the gene can obviously improve the salt and alkali tolerance of tomatoes and provides a new gene resource and technical scheme for molecular breeding of drought-resistant varieties of tomatoes. In order to achieve the aim of the invention, the invention adopts the following technical scheme: The LecRK gene is derived from tomato, the nucleotide sequence of the LecRK gene is shown as SEQ ID NO.1, and the nucleotide sequence of the LecRK gene is shown as SEQ ID NO. 2, and the regulation and control of the salt and alkali resistance of the tomato is specifically to improve the salt and alkali resistance of the tomato, namely the survivability and stress resistance of the tomato under drought stress can be obviously improved by enhancing the expression of LecRK gene. Further, the method is characterized in that LecRK genes are transferred into tomato plants, lecRK genes are subjected to over-expression, and the method for transferring LecRK genes into tomato plants is one of an agrobacterium infection method, a gene gun method or a polyethylene glycol method. Further, the application is to culture transgenic tomato varieties with enhanced saline-alkali tolerance. The invention also provides a recombinant expression vector for regulating and controlling the saline-alkali tolerance of tomatoes, and the nucleotide sequence of the recombinant expression vector is shown as SEQ ID NO. 3. The invention also provides a transgenic cell line or engineering bacteria containing the recombinant expression vector. The invention also provides a method for improving the saline-alkali tolerance of tomatoes, which comprises the following steps of 1) constructing a recombinant expression vector containing LecRK genes, 2) transferring the recombinant expression vector into tomato receptor cells to obtain transgenic tomato cells, 3) culturing the transgenic tomato cells into complete plants, and screening out transgenic tomato plants with over-expressed LecRK genes and enhanced saline-alkali tolerance. Further, the enhancement of the saline-alkali tolerance in the step 3) is characterized in that compared with wild tomatoes, the seed germination rate of the transgenic tomatoes under drought stress is improved by 25% -40%, the relative germination rate is improved by 20% -35%, the number of leaf pores is reduced by 15% -28%, and the leaf water loss rate is reduced by 20% -35%. The beneficial effects of the invention are as follows: The invention discovers and proves the important role of LecRK gene in the regulation of the salt and alkali tolerance of tomatoes for the first time, fills up the blank of the tomato