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CN-121992032-A - Application of PadPGR a gene in breeding fast-growing high-quality high-yield poplar

CN121992032ACN 121992032 ACN121992032 ACN 121992032ACN-121992032-A

Abstract

The invention discloses an application of PadPGR a gene in breeding fast-growing high-quality high-yield poplar, belongs to the technical field of genetic engineering, and particularly relates to an application of PadPGR a gene in breeding fast-growing high-quality high-yield poplar, wherein the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO. 1. According to the invention, through phenotypic analysis PadPGR a gene over-expression transgenic poplar plant height is obviously increased, net photosynthetic rate is obviously improved, and capability of resisting oxidative stress is obviously enhanced. The PadPGR a gene is an important forest rapid-growth improvement gene resource, and can provide an excellent transgenic new variety for the construction of rapid-growth high-quality high-yield poplar artificial forest by utilizing a genetic engineering technology means.

Inventors

  • YANG QI
  • HUANG JIN
  • Yang Kangbiao
  • TONG ZAIKANG

Assignees

  • 浙江农林大学

Dates

Publication Date
20260508
Application Date
20260331

Claims (9)

  1. The application of PadPGR5a gene in fast-growing high-quality high-yield poplar breeding is characterized in that the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the method is characterized in that through over-expression of PadPGR a gene, the high growth rate of poplar plants is improved, the leaf area of poplar is increased, the photosynthetic capacity of poplar is enhanced, the oxidation stress resistance of poplar is enhanced, and fast-growing high-quality high-yield poplar is cultivated.
  2. The application of PadPGR5a gene in the high regulation Yang Shuzhu is characterized in that the nucleotide sequence of the PadPGR a gene is shown as SEQ ID NO.1, and the growth rate of poplar plant height is improved by over-expressing PadPGR a gene.
  3. The application of PadPGR5a gene in regulation of poplar leaf growth is characterized in that the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the area of poplar leaf is increased by over-expression of PadPGR a gene.
  4. The application of PadPGR5a gene in regulation of the photosynthetic capacity of poplar is characterized in that the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the photosynthetic capacity of poplar is enhanced by over-expression of PadPGR a gene.
  5. 5. The use according to claim 4, wherein the specific leaf weight of the poplar plant is increased, the light energy capturing capacity of the leaf is enhanced and the photosynthesis capacity of the poplar is enhanced by over-expression of PadPGR a gene.
  6. The application of PadPGR5a gene in regulation and control of the oxidation stress resistance of poplar is characterized in that the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the oxidation stress resistance of poplar is enhanced by over-expression of PadPGR a gene.
  7. 7. The use according to claim 6, wherein the oxidation stress resistance of poplar is enhanced by reducing the production and accumulation of active oxygen by over-expression of PadPGR a gene.
  8. 8. The protein encoded by the PadPGR a gene as claimed in claim 1, wherein the amino acid sequence of the protein encoded by the PadPGR a gene is shown in SEQ ID No. 2.
  9. 9. The use of the protein encoded by PadPGR a gene according to claim 8 in breeding fast-growing high-quality high-yield poplar, wherein the protein encoded by PadPGR a gene is overexpressed, so that the growth rate of poplar plant height is increased, the leaf area of poplar is increased, the photosynthetic capacity of poplar is enhanced, the oxidation stress resistance of poplar is enhanced, and fast-growing high-quality high-yield poplar is cultivated.

Description

Application of PadPGR a gene in breeding fast-growing high-quality high-yield poplar Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to application of PadPGR a gene in fast-growing high-quality high-yield poplar breeding. Background The poplar is used as a global important fast-growing wood tree species and an ecological restoration tree species, and has irreplaceable value in the fields of forestry production, industrial materials, carbon sink and the like due to the characteristics of rapid growth, strong adaptability, wide wood application and the like. Along with the continuous increase of global wood demand and the continuous deep construction of ecological environment, the cultivation of new poplar varieties with the characteristics of rapid growth, excellent wood quality and high yield has become one of the core targets of modern forestry breeding. Traditional poplar breeding mainly depends on hybridization breeding and phenotype selection, and the method has the problems of long breeding period, difficult fine character aggregation, limited genetic gain and the like although remarkable effects are achieved. In recent years, with the rapid development of molecular biology and genomics, genetic engineering technology provides a new powerful tool for forest genetic improvement. Through the excavation and utilization of key functional genes, the precise regulation and control of the characters such as growth, materials, resistance and the like are realized, and the method becomes an important direction of poplar molecular design breeding. Photosynthesis is the substance and energy basis of biomass accumulation in the regulatory network of plant growth and development. Studies have shown that functional deletions of related genes in pattern plants such as Arabidopsis lead to reduced photosynthetic efficiency and increased sensitivity to strong light. However, in perennial woody plants such as poplar, there has been no study on related genes, and how genes affect the growth and development of trees, biomass formation and wood quality by regulating photosynthesis pathways has not been systematically elucidated. Therefore, based on the industrial demand and the blank of the prior art, aiming at the bottleneck that a plurality of complex characters are difficult to synergistically improve in the existing poplar breeding technology, a novel germplasm of a fast-growing high-quality high-yield poplar is created through a genetic engineering means, and the method has important theoretical and practical significance for promoting the technical progress of poplar breeding and improving the forestry production benefit. Yang Shugan is straight, high in yield, rapid in growth, short in rotation period and wide in distribution range, and is an important tree species for China. However, the common single-yield level of the poplar artificial forest is low, the breakthrough fine variety creation is insufficient, the industrial market demand is difficult to meet, and the development of the poplar industry in China is severely restricted. Aiming at the problems of low wood quality, insufficient quality varieties and the like, the novel poplar material with excellent properties is created by utilizing a genetic engineering means, and has important significance for cultivating novel varieties of fast-growing high-quality and high-yield woods. Disclosure of Invention In order to solve the technical problems, the invention provides an application of PadPGR a gene in breeding fast-growing high-quality high-yield poplar, which is used for breeding the fast-growing high-quality high-yield poplar, and the over-expression of PadPGR a gene enables the plant height of transgenic poplar to be obviously increased, the net photosynthetic rate to be obviously improved, and the capability of resisting oxidative stress to be obviously enhanced. In order to achieve the purpose, the invention provides an application of PadPGR a gene in fast-growing high-quality high-yield poplar breeding, and the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO. 1. Preferably, through over-expression PadPGR a genes, the growth speed of the poplar plant height is improved, the leaf area of the poplar is increased, the photosynthetic capacity of the poplar is enhanced, the oxidation stress resistance of the poplar is enhanced, and the fast-growing high-quality and high-yield poplar is cultivated. The invention also provides application of PadPGR a gene in Yang Shuzhu high regulation, the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the growth rate of poplar plant height is improved by over-expressing PadPGR a gene. The invention also provides an application of PadPGR a gene in regulating growth of poplar leaves, wherein the nucleotide sequence of PadPGR a gene is shown as SEQ ID NO.1, and the area of the poplar leaves is increased by over-expressing PadPGR a gene. The