CN-121992034-A - Application of OsZIP gene

Abstract

The invention provides application of OsZIP13 gene, osZIP gene is used for regulating and controlling salt and alkali stress tolerance of rice plants, osZIP gene in rice plants is used for enhancing the salt and alkali stress tolerance of rice plants by over-expression, osZIP gene in rice plants is/are edited by genes, the salt and alkali stress tolerance of rice plants is reduced, the nucleotide sequence of coding region of OsZIP gene is shown as SEQ ID No. 1, and the amino acid sequence of coding region of OsZIP gene is shown as SEQ ID No. 2. The gene is over-expressed in rice, so that the tolerance of the rice to the saline-alkali stress in germination and seedling stages can be enhanced, the mutant strain is more sensitive to the saline-alkali stress than the wild rice, and the discovery of the gene provides important gene resources and theoretical basis for cultivating the saline-alkali resistant plants, so that the gene has wide application prospect.

Inventors

• Shen Jiarongrong
• YU YANG
• GAO MEINA
• HAN ZIMING
• WANG ZIYU
• CHEN CHANGTING
• LIU FANGYUAN

Assignees

• 沈阳大学

Dates

Publication Date

20260508

Application Date

20260402

Claims (4)

1. 1. The application of OsZIP gene, which is characterized in that OsZIP gene is used for regulating and controlling the saline-alkali stress tolerance of rice plants; The OsZIP gene in the rice plant is over-expressed to enhance the saline-alkali stress tolerance of the rice plant; or/and gene OsZIP gene in gene editing rice plant for reducing salt and alkali stress tolerance of said rice plant; The nucleotide sequence of the OsZIP gene coding region is shown as SEQ ID No.1, and the amino acid sequence of the OsZIP gene coding region is shown as SEQ ID No. 2.
2. 2. The use of OsZIP gene according to claim 1, wherein when the OsZIP gene is overexpressed in rice plants, the root length of rice plants in germination period under salt-alkali stress is higher than that of wild rice plants; The fresh weight, plant height, root length and chlorophyll content of the rice plants under the saline-alkali stress at the seedling stage are all higher than those of the wild rice plants, and the MDA content is lower than that of the wild rice plants.
3. 3. The use of OsZIP gene according to claim 1, wherein when the OsZIP gene is genetically edited in a rice plant, a oszip mutant rice plant is obtained, and the plant height and root length of the oszip mutant rice plant under salt and alkali stress are both lower than those of a wild rice plant.
4. 4. The use of OsZIP gene as defined in claim 1, wherein the OsZIP gene overexpression vector is constructed for cultivating transgenic rice plants with high tolerance to saline-alkali stress.

Description

Application of OsZIP gene Technical Field The invention belongs to the technical field of biology, and particularly relates to application of OsZIP gene. Background Soil salinization refers to an environmental geological phenomenon formed by redistribution, excessive accumulation and diffusion of soluble salts in soil under unfavorable natural conditions such as climate, hydrology, topography, soil texture and the like, and not only causes farmland degradation and land desertification, but also severely restricts agricultural production and economic development. Iron is a trace element essential for plant growth and development, and plays an important role in respiration, photosynthesis, synthesis of protein and nucleic acid, and enzymatic reaction of plants. Iron deficiency can lead to hindered chlorophyll synthesis, manifesting as yellowing of new leaves, and in severe cases can affect crop yield and quality. Research shows that the saline-alkali soil can influence the absorption and transportation of iron ions by rice, and further influence the growth and development, yield and quality of the rice. Therefore, how to cultivate new rice varieties suitable for planting in saline-alkali soil by improving the absorption and transportation capacity of the rice to iron ions in the saline-alkali soil is a great problem to be solved in agricultural production. The ZIP family genes of the rice mainly participate in the transportation of zinc and iron ions, and part of the ZIP family genes are found to participate in responding to the saline-alkali stress response. Disclosure of Invention The technical problem to be solved by the invention is to provide an application of OsZIP gene aiming at the defects of the prior art, the gene is over-expressed in rice, the tolerance of the rice to the saline-alkali stress in germination and seedling stages can be enhanced, important gene resources and theoretical basis are provided for cultivating saline-alkali tolerant plants, and the application prospect is wide. In order to solve the technical problems, the technical scheme adopted by the invention is that the OsZIP gene is applied, and the OsZIP gene is used for regulating and controlling the saline-alkali stress tolerance of rice plants; The OsZIP gene in the rice plant is over-expressed to enhance the saline-alkali stress tolerance of the rice plant; or/and gene OsZIP gene in gene editing rice plant for reducing salt and alkali stress tolerance of said rice plant; The nucleotide sequence of the OsZIP gene coding region is shown as SEQ ID No.1, and the amino acid sequence of the OsZIP gene coding region is shown as SEQ ID No. 2. Preferably, when OsZIP gene in the rice plant is overexpressed, the root length of the rice plant in the germination period under the saline-alkali stress is higher than that of the wild rice plant; The fresh weight, plant height, root length and chlorophyll content of the rice plants under the saline-alkali stress at the seedling stage are all higher than those of the wild rice plants, and the MDA content is lower than that of the wild rice plants. Preferably, when the OsZIP gene in the rice plant is genetically edited, a oszip mutant rice plant is obtained, and the plant height and root length of the oszip mutant rice plant under saline-alkali stress are lower than those of a wild rice plant. Preferably, the OsZIP gene over-expression vector is constructed and used for cultivating transgenic rice plants with high saline-alkali stress tolerance. Compared with the prior art, the invention has the following advantages: according to the invention, through homologous cloning, a plant expression vector of OsZIP gene is constructed, osZIP gene is over-expressed in rice to obtain a transgenic plant, meanwhile, a CRISPR/Cas9 mutant is constructed, a rice zip13 mutant is obtained through a gene editing method, and the saline-alkali resistance of OsZIP gene is verified in detail by utilizing an over-expressed strain and a mutant strain. Experiments prove that OsZIP gene has stronger alkali resistance, the tolerance of rice to saline-alkali stress in germination and seedling stages can be obviously enhanced by over-expressing the gene in rice, and compared with wild rice, a mutant strain is more sensitive to saline-alkali stress, and the discovery of the gene provides important gene resources and theoretical basis for cultivating saline-alkali resistant plants, so that the gene has wide application prospect. The invention is described in further detail below with reference to the drawings and examples. Drawings FIG. 1 is a cloning map of rice OsZIP gene in example 1 of the present invention. FIG. 2 is a graph showing the analysis of the expression level of OsZIP gene in various organs of rice in example 1 according to the present invention. FIG. 3 is a diagram showing the expression pattern of rice OsZIP gene under salt and alkali stress in example 1 of the present invention. FIG. 4 is an analysis of the exp