CN-121992036-A - Use of GhQUAC1 related biological products in regulating plant stress resistance

Abstract

The invention belongs to the technical field of plant biology, and particularly relates to application of GhQUAC1 related biological products in regulating plant stress resistance, wherein GhQUAC is any one of an amino acid sequence shown as SEQ ID NO.1, a sequence formed by 225-541 th amino acid in SEQ ID NO.1, an amino acid sequence obtained by connecting tag protein at the N end or/and the C end of A, an amino acid sequence obtained by connecting tag protein at the N end or/and the C end of B, and an amino acid sequence which is obtained by substituting, deleting and/or adding amino acid residues of SEQ ID NO.1 and has more than 67% identity and same function as the SEQ ID NO. 1. The invention proves that GhQUAC is a positive regulation key gene of plant stress resistance, and provides a new gene target and theoretical basis for analyzing molecular mechanism of plant stress response.

Inventors

• LI FANGJUN
• SUI YIPING
• TIAN XIAOLI
• LI ZHAOHU

Assignees

• 中国农业大学

Dates

Publication Date

20260508

Application Date

20260326

Claims (8)

1. Application of GhQUAC1 related biological products in regulating plant stress resistance, characterized in that GhQUAC is any one of the following: a is the amino acid sequence shown in SEQ ID NO. 1; b is a sequence formed by 225 th to 541 th amino acid in SEQ ID NO. 1; c, connecting an amino acid sequence obtained by the tag protein at the N end or/and the C end of the A; d, connecting the N end or/and the C end of the B with an amino acid sequence obtained by the tag protein; E, based on SEQ ID NO.1, carrying out substitution, deletion and/or addition of amino acid residues, and obtaining an amino acid sequence which has more than 67% of sequence identity and identical function with SEQ ID NO. 1.
2. 2. The use according to claim 1, wherein the stress resistance is any one of the following: 1) Drought resistance; 2) Osmotic stress resistance; 3) NO mediated resistance.
3. 3. The use of claim 1, wherein the tag protein comprises at least one of a Flag tag, his tag, MBP tag, HA tag, myc tag, GST tag, and SUMO tag.
4. 4. The use of claim 1, wherein the biologic is any one of the following: a) A nucleic acid molecule encoding GhQUAC 1; b) An expression cassette comprising a) said nucleic acid molecule; c) A recombinant vector comprising a) said nucleic acid molecule or a recombinant vector comprising b) said expression cassette; d) A recombinant microorganism comprising a) said nucleic acid molecule, a recombinant microorganism comprising b) said expression cassette or a recombinant microorganism comprising c) said recombinant vector; e) A transgenic plant cell line comprising a) said nucleic acid molecule, a transgenic plant cell line comprising b) said expression cassette or a transgenic plant cell line comprising c) said recombinant vector; f) Transgenic plant tissue comprising a) said nucleic acid molecule, transgenic plant tissue comprising b) said expression cassette or transgenic plant tissue comprising c) said recombinant vector; g) A transgenic plant organ comprising a) said nucleic acid molecule, a transgenic plant organ comprising b) said expression cassette or a transgenic plant organ comprising c) said recombinant vector; h) A transgenic plant comprising a) said nucleic acid molecule, a transgenic plant comprising b) said expression cassette or a transgenic plant comprising c) said recombinant vector; i) A tissue culture produced by the regenerable cells of the transgenic plant of h); j) Protoplasts produced from i) the tissue culture; k) A recombinant vector for enhancing GhQUAC1 expression level, a recombinant vector for enhancing GhQUAC1 activity, or a recombinant vector for increasing GhQUAC1 content; l) a recombinant microorganism which enhances the expression level of GhQUAC, a recombinant microorganism which enhances the activity of GhQUAC1 or a recombinant microorganism which increases the GhQUAC content; m) a recombinant vector which suppresses the amount of GhQUAC expression, a recombinant vector which suppresses GhQUAC1 activity, or a recombinant vector which reduces GhQUAC1 content; n) a recombinant microorganism which inhibits GhQUAC of the expression level, a recombinant microorganism which inhibits GhQUAC1 activity or a recombinant microorganism which reduces GhQUAC content.
5. 5. The use according to claim 4, wherein the recombinant microorganism is any one of yeast, bacteria, algae and fungi; the transgenic plant organ is any one of the root, stem, leaf, flower, fruit and seed of the transgenic plant; The tissue culture is derived from any one of roots, stems, leaves, flowers, fruits, seeds, pollen, embryos, and anthers.
6. 6. The use of claim 4, wherein the nucleic acid molecule is DNA or RNA; the DNA includes any one of cDNA, genomic DNA and recombinant DNA; The RNA includes any one of mRNA and hnRNA.
7. 7. The use according to claim 1, wherein the plant is any one of the following: monocotyledonous plants; Dicotyledonous plants; III, cruciferous plants; IV, cotton plants; v, cotton; VI, arabidopsis thaliana.
8. 8. The application according to claim 1, wherein the method of application is: The genetic transformation technology is utilized to change the expression quantity of GhQUAC < 1 > in the plant so as to realize the regulation of the stress resistance of the plant.

Description

Use of GhQUAC1 related biological products in regulating plant stress resistance Technical Field The invention belongs to the technical field of plant biology, and particularly relates to an application of GhQUAC a related biological product in regulating and controlling plant stress resistance. Background Drought is a frequent, durable and widely influenced natural disaster, seriously threatens 26% of cultivated land area of the whole world, especially under the climate warming background, and extremely frequent drought forms a serious challenge for the production of crops such as cotton, corn and the like. Abiotic stresses such as drought lead to reduced cotton yield and quality. The shortage of water resources is a main factor limiting cotton production, and how to effectively reduce the influence of drought on cotton growth in drought areas becomes a current problem to be solved urgently. At present, functional verification of only a few genes related to drought tolerance in cotton is reported. Therefore, the target genes for regulating and controlling the cotton stress resistance are fully excavated, and the molecular mechanism for resolving the cotton drought resistance provides excellent targets for the cotton drought resistance, and is vital to guaranteeing the cotton production under extreme weather background. Disclosure of Invention The invention aims to provide an application of GhQUAC1 related biological products in regulating and controlling plant stress resistance, and solves the problems in the prior art. The technical scheme adopted by the invention is as follows: The invention provides an application of GhQUAC1 related biological products in regulating plant stress resistance, wherein GhQUAC1 refers to any one of the following: a is the amino acid sequence shown in SEQ ID NO. 1; b is a sequence formed by 225 th to 541 th amino acid in SEQ ID NO. 1; c, connecting an amino acid sequence obtained by the tag protein at the N end or/and the C end of the A; d, connecting the N end or/and the C end of the B with an amino acid sequence obtained by the tag protein; E, based on SEQ ID NO.1, carrying out substitution, deletion and/or addition of amino acid residues, and obtaining an amino acid sequence which has more than 67% of sequence identity and identical function with SEQ ID NO. 1. Preferably, the stress resistance means any one of the following: 1) drought resistance, 2) osmotic stress resistance, 3) NO-mediated resistance. Preferably, the tag protein refers to a polypeptide or protein which is fusion expressed with the target protein by using a DNA in vitro recombination technology, so as to facilitate the expression, detection, tracing or purification of the target protein. The tag protein comprises at least one of a Flag tag, a His tag, an MBP tag, an HA tag, a myc tag, a GST tag and a SUMO tag. Identity refers to the identity of amino acid sequences. The identity of amino acid sequences may be determined using sequence alignment software or homology search sites on the internet, e.g., the identity of amino acid sequences may be determined using sequence alignment software DNAMAN. For example, the amino acid sequence shown in SEQ ID NO.1 and the sequence to be examined may be introduced into software, and a rapid alignment, gap penalty, top diagonal number may be selected by using a multiple sequence alignment function. And in the output sequence file, two sequences are selected for comparison, the Identity of the amino acid sequence to be detected is calculated, and then the Identity value Identity can be obtained. The 67% identity or more may be at least 67% to 100% identity of any value. Preferably, the biologic is any one of the following: a) A nucleic acid molecule encoding GhQUAC 1; b) An expression cassette comprising a) said nucleic acid molecule; c) A recombinant vector comprising a) said nucleic acid molecule or a recombinant vector comprising b) said expression cassette; d) A recombinant microorganism comprising a) said nucleic acid molecule, a recombinant microorganism comprising b) said expression cassette or a recombinant microorganism comprising c) said recombinant vector; e) A transgenic plant cell line comprising a) said nucleic acid molecule, a transgenic plant cell line comprising b) said expression cassette or a transgenic plant cell line comprising c) said recombinant vector; f) Transgenic plant tissue comprising a) said nucleic acid molecule, transgenic plant tissue comprising b) said expression cassette or transgenic plant tissue comprising c) said recombinant vector; g) A transgenic plant organ comprising a) said nucleic acid molecule, a transgenic plant organ comprising b) said expression cassette or a transgenic plant organ comprising c) said recombinant vector; h) A transgenic plant comprising a) said nucleic acid molecule, a transgenic plant comprising b) said expression cassette or a transgenic plant comprising c) said recombinant vector; i) A tissue culture pro