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CN-121992044-A - Preparation method of diglyceride with uric acid reducing effect

CN121992044ACN 121992044 ACN121992044 ACN 121992044ACN-121992044-A

Abstract

The invention relates to the technical field of diglyceride preparation, in particular to a method for preparing diglyceride with uric acid reducing effect. The method comprises the steps of (1) carrying out partial hydrolysis on vegetable oil by using a complex enzyme composed of lipase and cutinase, dehydrating to obtain a hydrolysate, wherein the content of free fatty acid in the hydrolysate is 37.0-46.0 wt%, adding glycerol and lipase into the hydrolysate obtained in the step (1) for reaction, adding water to remove superfluous glycerol, standing for layering, collecting supernatant, and distilling for separation to obtain the edible diglyceride. The content of diglyceride in the final product is up to more than 80% by strictly controlling the enzyme composition and free fatty acid in the hydrolysis and esterification processes, the reaction process is carried out according to the hydrolysis and esterification sequence, the process steps are simple, no organic solvent is used in the whole reaction process, the environment-friendly effect is realized, and the obtained diglyceride has remarkable uric acid reducing effect.

Inventors

  • LI SHAOJUN
  • GUO FEILONG
  • WU DEHONG
  • GUO RUIYONG

Assignees

  • 濮阳市益佳康健康科技有限公司

Dates

Publication Date
20260508
Application Date
20260324

Claims (10)

  1. 1. A method for preparing diglyceride with uric acid reducing effect, which is characterized by comprising the following steps: (1) Carrying out partial hydrolysis on vegetable oil by adopting a complex enzyme catalytic hydrolysis method consisting of lipase and cutinase, and dehydrating to obtain a hydrolysate, wherein the content of free fatty acid in the hydrolysate is 37.0-46.0 wt%; (2) Adding glycerol and lipase into the hydrolysate obtained in the step (1) for reaction, adding water to remove superfluous glycerol, standing for layering, collecting supernatant, and distilling for separation to obtain the edible diglyceride.
  2. 2. The method according to claim 1, wherein the mass ratio of cutinase to lipase in the complex enzyme of step (1) is 1..2 to 5.
  3. 3. The preparation method according to claim 1, wherein the complex enzyme in the step (1) is added in an amount of 0.5% -1.2% by mass of the edible oil.
  4. 4. The method according to claim 1, wherein the water-to-oil ratio in the hydrolysis in the step (1) is 2-3..1 g/mL, the reaction temperature is 20-40 ℃ and the reaction time is 2-5h.
  5. 5. The method according to claim 1, wherein the vegetable oil in step (1) is at least one selected from the group consisting of acer truncatum seed oil, seabuckthorn seed oil, canola oil, peony seed oil, corn oil, peanut oil, linseed oil, safflower seed oil and silybum marianum seed oil.
  6. 6. The method according to claim 1, wherein the glycerol is added in the amount of 2 to 3 times the mass of the hydrolysate in the step (2), and the lipase is added in the amount of 0.1 to 0.5% by mass of the hydrolysate in the step (1).
  7. 7. The method according to claim 1, wherein the enzyme used in the step (1) or the step (2) is an immobilized enzyme, and the enzyme is recovered after the completion of the catalytic reaction.
  8. 8. The process according to claim 1, wherein the reaction in step (2) is carried out at 30 to 45 ℃ for 2 to 6 hours.
  9. 9. The method according to claim 1, wherein the time of the standing in the step (2) is 20 to 40 minutes.
  10. 10. The method according to claim 1, wherein the conditions for distillation separation in the step (2) are cooling water temperature of 45-55 ℃, vacuum degree of 0.3-0.5Pa, film scraping rate of 90-110r/min, and feeding flow rate of 5-12kg/min.

Description

Preparation method of diglyceride with uric acid reducing effect Technical Field The invention belongs to the technical field of diglyceride preparation, and particularly relates to a preparation method of diglyceride with uric acid reducing effect. Background Diglycerides, also known as diglycerides, diacylglycerols, are known as DAG, are a multifunctional additive, and in 1988, yasukawa and his research group developed a special edible fat with DAG as the main ingredient for weight loss. In 1997, the japanese flower king was continuously developed in the field of diglycerides, and a plurality of patents on the preparation method of diglycerides and the composition of diglycerides were filed in japan, the united states, and china, respectively. In addition, the korean first sugar industry has introduced diglyceride products having weight-losing effects in 2006. In 2021, 7 months, the new food material termination review catalogue by national Wei Jian has the physical and chemical effects of diglyceride oil, and the diglyceride oil is taken as a new food material. The medical society for elderly people in China established the body standard T/GGSS020-2021 for edible vegetable oil diglyceride oils for elderly people, suitable for edible vegetable oil diglyceride oils for elderly people, 6 months 2021. At present, the preparation method of diglyceride is mainly divided into a chemical method and a biological enzyme method, and compared with the chemical method, the biological enzyme method has the advantages of mild reaction condition, low energy consumption, no pollution and less side reaction, so that the biological enzyme method is a green and environment-friendly synthesis process. The prior art Chinese patent application CN105400837A discloses a preparation method of enzyme-catalyzed diglyceride, which comprises the following steps of (1) partially hydrolyzing edible oil by adopting an enzyme-catalyzed hydrolysis method, dehydrating to obtain a hydrolysate, enabling the content of the diglyceride in the hydrolysate to be 30.0-35.0 wt% and the content of free fatty acid to be 26.0-30.0 wt%, and (2) re-esterifying, namely adding 2.5-4 times of glycerol into the hydrolysate obtained in the step (1), and carrying out esterification reaction by adopting an enzyme-catalyzed esterification method to synthesize the diglyceride, and removing the superfluous glycerol to obtain the edible diglyceride. The Chinese patent application CN119932125A discloses a method for preparing diglyceride for reducing uric acid by enzymolysis, and a product and application thereof, wherein the method comprises the following steps of (1) mixing vegetable oil, glycerol, water and enzyme preparation for reaction to obtain reaction liquid, (2) standing and layering the reaction liquid, collecting supernatant, (3) distilling and separating the supernatant, decoloring and deodorizing the supernatant to obtain diglyceride, wherein the enzyme preparation in the step (1) comprises Lipase, esterase Esterase and phospholipase C in a weight ratio of 5-10:0.1-0.3:1, but the content of the diglyceride in the product of the patent application is still to be further improved. Therefore, there is a need to develop a preparation method of diglycerides with uric acid reducing effect, which has simple preparation steps and high content of diglycerides in the product. Disclosure of Invention Compared with the prior art, the invention provides the preparation method of diglyceride with uric acid reducing effect, the content of diglyceride in the final product is more than 80% by strictly controlling the enzyme composition and free fatty acid in the hydrolysis and esterification processes, the reaction process is carried out in the hydrolysis and esterification sequence, the process steps are simple, and the whole reaction process does not use organic solvents, so that the environment is protected. The invention is realized by the following technical scheme: The first aspect of the invention relates to a preparation method of diglyceride with uric acid reducing effect, which comprises the following steps: (1) Carrying out partial hydrolysis on vegetable oil by adopting a complex enzyme catalytic hydrolysis method consisting of lipase and cutinase, and dehydrating to obtain a hydrolysate, wherein the content of free fatty acid in the hydrolysate is 37.0-46.0 wt%; (2) Adding glycerol and lipase into the hydrolysate obtained in the step (1) for reaction, adding water to remove superfluous glycerol, standing for layering, collecting supernatant, and distilling for separation to obtain the edible diglyceride. Preferably, the mass ratio of cutinase to lipase in the complex enzyme of step (1) is 1..2-5. Preferably, the adding amount of the complex enzyme in the step (1) is 0.5% -1.2% of the mass of the edible oil. Preferably, the oil-water ratio in the hydrolysis of step (1) is 2-3..1 g/mL. Preferably, the reaction temperature of the reaction in step (1) is 20-