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CN-121992048-A - Bagasse enzymatic hydrolysate detoxification process and application thereof in xanthan gum production

CN121992048ACN 121992048 ACN121992048 ACN 121992048ACN-121992048-A

Abstract

A bagasse enzymatic hydrolysate detoxification process and application thereof in xanthan gum production relate to the technical fields of bagasse enzymatic hydrolysate treatment and xanthan gum production. The bagasse enzymatic hydrolysate detoxification process adopts a quicklime detoxification method or a calcium phosphate detoxification method to treat the bagasse enzymatic hydrolysate, and removes weak acid components, phenolic substances and furfural substances in the bagasse enzymatic hydrolysate. Adding quantitative calcium oxide solid into bagasse enzymatic hydrolysate, continuously stirring, standing, primarily filtering to remove solid, introducing carbon dioxide gas to ensure that the pH value of filtrate is less than or equal to 8, secondarily filtering to remove solid, and sterilizing the filtrate at high temperature. The detoxification process can efficiently remove the inhibitors such as organic acid and/or inorganic acid, phenols and furfural in the bagasse enzymatic hydrolysate, reduce the inhibition of fermentation growth of Xanthomonas campestris, remarkably improve the yield of the Xanthan gum, provide a new application direction for the bagasse enzymatic hydrolysate, provide a new fermentation raw material for the Xanthan gum, and reduce the production cost.

Inventors

  • ZHANG HONGTAO
  • Wan Zecheng
  • LIU DENGFENG

Assignees

  • 江南大学

Dates

Publication Date
20260508
Application Date
20260128

Claims (10)

  1. 1. A process for detoxication of bagasse enzymatic hydrolysate is characterized in that the bagasse enzymatic hydrolysate is treated by a quicklime detoxication method or a calcium phosphate detoxication method, and weak acid components, phenolic substances and furfural substances in the bagasse enzymatic hydrolysate are removed.
  2. 2. The process for detoxication of bagasse enzymatic hydrolysate as claimed in claim 1, wherein the method for detoxication by quicklime comprises adding a fixed amount of calcium oxide solid into the bagasse enzymatic hydrolysate, continuously stirring, standing, primarily filtering to remove the solid, introducing carbon dioxide gas to make pH of the filtrate less than or equal to 8, secondarily filtering to remove the solid, and sterilizing the filtrate at high temperature to obtain the bagasse enzymatic hydrolysate.
  3. 3. A bagasse enzymatic hydrolysate detoxification process as claimed in claim 2, wherein the quicklime detoxification process comprises the steps of: S1, neutralizing and detoxication, namely adding calcium oxide solids into bagasse enzymatic hydrolysate according to the addition amount of 2-3.0 g/L, continuously stirring for more than 6 h, standing, and primarily filtering to remove the solids; And S2, introducing CO 2 to finely adjust the pH, introducing carbon dioxide gas to enable the pH of the filtrate obtained by filtering the S1 to reach 5.87-8, filtering again to remove solids, and sterilizing the filtrate at a high temperature of 115 ℃ and 20 min to obtain the bagasse enzymolysis detoxification liquid.
  4. 4. A bagasse enzymatic hydrolysate detoxification process as claimed in claim 3, wherein in S1 the amount of calcium oxide solids added is 2.40g/L.
  5. 5. The process for detoxication of bagasse enzymatic hydrolysate as recited in claim 4, wherein in S2, the amount of CO 2 introduced is controlled so that the pH of the filtrate obtained by filtering S1 is less than or equal to 7.
  6. 6. A bagasse enzymatic hydrolysate detoxification process as claimed in claim 5, wherein in S2 the amount of CO 2 introduced is controlled to achieve a pH of 5.87-6.0 in the filtrate obtained by filtration of S1.
  7. 7. The process for detoxication of bagasse enzymatic hydrolysate according to claim 1, wherein the specific operation of the calcium phosphate detoxication method is that the bagasse enzymatic hydrolysate is adjusted to have a pH value of less than or equal to 3 by phosphoric acid, and then is subjected to pH value=7 by solid CaO, and then is left for more than 1h, calcium phosphate solid is removed by suction filtration, and the filtrate is sterilized at high temperature to obtain the bagasse enzymatic hydrolysate.
  8. 8. An application of a bagasse enzymatic hydrolysate detoxification process in xanthan gum production, which is characterized in that the bagasse enzymatic hydrolysate prepared by the bagasse enzymatic hydrolysate detoxification process as claimed in any one of claims 1-7 is used as a fermentation substrate to produce xanthan gum.
  9. 9. The use of the bagasse enzymatic hydrolysate detoxification process according to claim 8 in xanthan gum production, wherein xanthomonas campestris is added into the bagasse enzymatic hydrolysate prepared by the bagasse enzymatic hydrolysate detoxification process, fermentation culture is carried out for more than 96 hours, then the fermentation broth is centrifuged to obtain supernatant, alcohol precipitation is carried out, and sediment obtained by decompression filtration is washed and dried, thus obtaining the xanthan gum.
  10. 10. Use of the bagasse enzymatic hydrolysate detoxification process according to claim 9 in the production of xanthan gum, wherein the added amount of xanthomonas campestris is 10% (V/V) of the bagasse enzymatic hydrolysate.

Description

Bagasse enzymatic hydrolysate detoxification process and application thereof in xanthan gum production Technical Field The invention relates to the technical field of bagasse enzymatic hydrolysate treatment and xanthan gum production, in particular to a bagasse enzymatic hydrolysate detoxification process and application thereof in xanthan gum production. Background By 2025, the scale of the biological manufacturing industry in China has reached the trillion yuan scale, the global biological manufacturing industry is expected to create economic value of 30 trillion dollars by 2050, and the economic value is one third of the global manufacturing industry, and the carbon source/nitrogen source of biological manufacturing is mainly from grain deep processing products. At present, the import of grains in China exceeds 1 hundred million tons each year, and the demand of fermentation industry in China reaches 2 hundred million tons of grains, and microbial polysaccharide including xanthan gum is mainly obtained by fermenting grains as biomass carbon sources. At present, the grain crisis is increasingly serious, and how to realize the replacement of non-grain biomass of grain biomass in the biological manufacturing industry including the microbial polysaccharide industry has important significance for developing biological manufacturing circular economy and guaranteeing the grain and ecological safety of China. Bagasse, which is a typical non-grain biomass carbon source as a solid waste in the sugar industry, is composed mainly of cellulose, hemicellulose and lignin, and has an annual yield of about 1100 ten thousand tons, rich in fermentable sugars. The bagasse can be used for efficiently converting cellulose and hemicellulose in the bagasse into fermentable sugar (mainly glucose and xylose) through pretreatment and enzymolysis, is a non-grain biomass carbon source with great potential, and can be used as a carbon source for microbial fermentation. Although bagasse enzymatic hydrolysate has a very broad potential prospect as a carbon source for biological manufacture, there is a great challenge in practical use that after bagasse is converted into enzymatic hydrolysate, the conversion of bagasse into enzymatic hydrolysate, which also contains aliphatic acids, esters (acetates), phenolic compounds (different compounds obtained from lignin hydrolysis) and products of sugar dehydration, including furan aldehydes furfural and 5-hydroxymethylfurfural (5-HMF), can severely affect microbial growth and product synthesis. Currently, there are a number of well-established pretreatment processes for fibrous and hemicellulose materials to hydrolyze to give sugars, which can be used for fermentative production of biofuels, as disclosed in CN104254613a, which relates to a process for detoxification of hydrolysates obtained from lignocellulosic biomass and a process for producing ethanol from said detoxified hydrolysates, said process comprising the steps of (a) mixing a starting solution of lignocellulosic hydrolysates obtained from lignocellulosic biomass with an amount of a first base or a mixture of first bases sufficient to raise the pH of said solution to between 3 and 8, said starting solution comprising a mixture of fermentable sugars, furan aldehydes and aliphatic acids, and (b) mixing the solution produced in step (a) with an amount of a second base or a mixture of second bases sufficient to raise the pH of said solution to between 7 and 10, said mixing being for a time sufficient to eliminate at least 40% of furan aldehydes in said lignocellulosic hydrolysates, thereby reducing the toxicity of said cellulosic hydrolysates. However, the method is complex to operate, and whether the detoxification method can efficiently remove the inhibitor of xanthan gum production strain Xanthomonas campestris in bagasse enzymatic hydrolysate can not be determined. Therefore, the method optimizes the detoxification process of fermentable sugar in the non-grain biomass source bagasse enzymatic hydrolysate, explores the feasibility and the efficiency of converting xanthan gum by using the bagasse enzymatic hydrolysate fermentation method, and has remarkable significance. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a novel, efficient and safe bagasse enzymatic hydrolysate detoxification process, and also discloses application of the detoxified bagasse fermentation broth serving as a substrate in preparation of xanthan gum. The technical scheme adopted for solving the technical problems is as follows: A process for detoxication of the enzymatic liquid of bagasse features that the enzymatic liquid of bagasse is treated by quick lime detoxication or calcium phosphate detoxication to remove weak acid, phenol and furfuraldehyde. The quick lime detoxification method comprises the specific operation steps of adding quantitative calcium oxide solid into bagasse enzymatic hydrolysate, continuously stirr