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CN-121992049-A - Rice bran feruloyl oligosaccharides and preparation method and application thereof

CN121992049ACN 121992049 ACN121992049 ACN 121992049ACN-121992049-A

Abstract

The invention relates to the technical field of biological medicine, in particular to rice bran feruloyl oligosaccharides, and a preparation method and application thereof. The invention provides a preparation method of rice bran feruloyl oligosaccharides, which comprises the following steps of mixing rice bran and normal hexane, degreasing, pasting, and carrying out enzymolysis to obtain rice bran insoluble dietary fibers, carrying out enzymolysis and centrifugation to the rice bran insoluble dietary fibers, taking supernatant, and carrying out gradient alcohol precipitation on the supernatant by using 50% ethanol, 70% ethanol and 90% ethanol in sequence to obtain the rice bran feruloyl oligosaccharides. The obtained rice bran feruloyl oligosaccharide has the effects of inhibiting the activities of alpha-amylase and alpha-glucosidase, and can be used as food-borne hypoglycemic component for developing functional foods, medicines and the like for regulating blood sugar.

Inventors

  • LIU KUNLUN
  • Chang Mengwei
  • LI QINGYUN
  • LIU YING
  • ZHU GUANGSU

Assignees

  • 河南工业大学

Dates

Publication Date
20260508
Application Date
20260213

Claims (10)

  1. 1. A preparation method of rice bran feruloyl oligosaccharides is characterized by comprising the following steps: (1) Mixing rice bran and normal hexane for degreasing to obtain defatted rice bran; (2) Pasting and carrying out enzymolysis on defatted rice bran to obtain insoluble dietary fiber of rice bran; (3) Performing enzymolysis and centrifugation on the insoluble dietary fiber of rice bran, collecting supernatant, and performing gradient ethanol precipitation on the supernatant sequentially with 50% ethanol, 70% ethanol and 90% ethanol to obtain the feruloyl oligosaccharide of rice bran.
  2. 2. The preparation method of the rice bran-hexane composite material is characterized in that in the step (1), the mass-volume ratio of rice bran to normal hexane is 250 mg:450-550 mL, the mixing degreasing time is 12-14 h, stirring is required in the mixing degreasing process, the stirring rotating speed is 160-180 rpm, and the degreasing times are 1-3.
  3. 3. The preparation method according to claim 1, wherein in the step (2), the step of gelatinizing is to mix defatted rice bran with water, adjust the pH to 5.5-6.5, and gelatinize for 8-12 min at 90-100 ℃ to obtain gelatinized defatted rice bran; the mass volume ratio of the defatted rice bran to the water is 50 mg:450-550 mL.
  4. 4. The method according to claim 3, wherein in the step (2), the enzymatic hydrolysis comprises the step of subjecting the gelatinized defatted rice bran to mixed enzymatic hydrolysis with a high temperature resistant alpha-amylase, an alkaline protease and an amyloglucosidase in this order.
  5. 5. The preparation method of the rice bran powder according to claim 4, wherein the addition amount of the high temperature resistant alpha-amylase is 4% of the mass of defatted rice bran, and the enzymolysis conditions of the defatted rice bran and the high temperature resistant alpha-amylase are that the enzymolysis is carried out for 25-35 min at 90-100 ℃; The addition amount of the alkaline protease is 2% of the mass of the defatted rice bran, and the enzymolysis condition of the alkaline protease is that the pH is adjusted to 8-9, and the alkaline protease is subjected to enzymolysis for 1.5-2.5 hours at 45-55 ℃; the addition amount of the amyloglucosidase is 2% of the mass of the defatted rice bran, and the enzymolysis condition of the amyloglucosidase is that the pH is adjusted to 4-5, and the amyloglucosidase is subjected to enzymolysis for 0.5-1.5 h at 50-60 ℃.
  6. 6. The preparation method of the rice bran enzyme preparation method is characterized in that in the step (3), the rice bran insoluble dietary fiber and xylanase liquid are subjected to enzymolysis for 12-14 hours at 50-60 ℃; The mass volume ratio of the rice bran insoluble dietary fiber to the xylanase liquid is 100 mg/1000 mL.
  7. 7. The rice bran feruloyl oligosaccharides prepared by the preparation method of any one of claims 1-6.
  8. 8. Use of the rice bran feruloyl oligosaccharides prepared by the preparation method of any one of claims 1 to 6 or the rice bran feruloyl oligosaccharides of claim 7 in the preparation of products for inhibiting the activities of alpha-amylase and alpha-glucosidase.
  9. 9. The application of the rice bran feruloyl oligosaccharides prepared by the preparation method of any one of claims 1-6 or the rice bran feruloyl oligosaccharides of claim 7 in the preparation of blood sugar regulating products.
  10. 10. The application of the rice bran feruloyl oligosaccharides prepared by the preparation method of any one of claims 1-6 or the rice bran feruloyl oligosaccharides of claim 7 in preparing products for treating diabetes.

Description

Rice bran feruloyl oligosaccharides and preparation method and application thereof Technical Field The invention relates to the technical field of biological medicine, in particular to rice bran feruloyl oligosaccharides, and a preparation method and application thereof. Background Diabetes is a global metabolic disease characterized by chronic hyperglycemia, whose morbidity and mortality rate rise year by year, severely threatening the health of humans. Among them, the control of postprandial blood glucose is a key link in diabetes management. Alpha-amylase can hydrolyze alpha-1, 4-glycosidic bond in starch to degrade polysaccharide into oligosaccharide, and alpha-glucosidase is responsible for decomposing oligosaccharide and disaccharide into absorbable monosaccharide, thus leading to rapid rise of postprandial blood sugar. Therefore, inhibition of digestive enzyme activity, delay of digestion and absorption of carbohydrates, is considered as one of the important strategies for effectively controlling postprandial blood glucose. At present, digestive enzyme inhibitors such as acarbose, voglibose and the like which are widely used clinically have definite curative effects, but often have gastrointestinal side effects such as abdominal distension, exhaustion, diarrhea and the like in the using process, so that the clinical application of the digestive enzyme inhibitors is limited to a certain extent. Therefore, the search for efficient, low-toxic digestive enzyme inhibitors from natural sources has become a hotspot in current drug development and functional food development. Rice bran is a major byproduct in the processing of rice and is rich in proteins, lipids, vitamins and various bioactive substances. For a long time, rice bran has been used as animal feed or discarded, and its high added value has not been fully developed, resulting in great waste of resources. Polysaccharides are important biological macromolecules, which are widely present in animals, plants and microorganisms, and are attracting attention because of their various biological activities such as immunoregulation, antitumor, antioxidant, and blood glucose lowering. In recent years, studies have reported that polysaccharides extracted from rice bran have a certain biological activity, such as antioxidant and immunopotentiating activities. However, the prior art research on rice bran polysaccharides has focused on their conventional extraction process and basic physiological functions, and has not been conducted intensively and systematically for their specific inhibition of alpha-amylase and alpha-glucosidase activities. In particular, there is still a lack of clear technical guidance as to how to obtain rice bran polysaccharide components with significantly enhanced alpha-glucosidase inhibitory activity by specific, optimized preparation methods (e.g., extraction, isolation, purification processes). The rice bran polysaccharide obtained in the prior art is often not ideal in inhibition activity, and the application of the rice bran polysaccharide in functional foods or medicines for reducing blood sugar is limited. Therefore, the field is in urgent need of a method for specifically preparing a product with high inhibition activity to digestive enzymes, and defining a preparation method and application thereof, so as to fill the blank of the prior art, realize high-value utilization of rice bran resources, and provide a new potential solution derived from natural products for preventing and treating diabetes. Disclosure of Invention The invention aims to provide rice bran feruloyl oligosaccharides, a preparation method and application thereof, wherein the rice bran feruloyl oligosaccharides can remarkably inhibit the activities of alpha-amylase and alpha-glucosidase. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a preparation method of rice bran feruloyl oligosaccharides, which comprises the following steps: (1) Mixing rice bran and normal hexane for degreasing to obtain defatted rice bran; (2) Pasting and carrying out enzymolysis on defatted rice bran to obtain insoluble dietary fiber of rice bran; (3) Performing enzymolysis and centrifugation on the insoluble dietary fiber of rice bran, collecting supernatant, and performing gradient ethanol precipitation on the supernatant sequentially with 50% ethanol, 70% ethanol and 90% ethanol to obtain the feruloyl oligosaccharide of rice bran. Preferably, in the step (1), the mass-volume ratio of the rice bran to the n-hexane is 250 mg:450-550 mL, the mixing degreasing time is 12-14 h, stirring is required in the mixing degreasing process, the stirring rotating speed is 160-180 rpm, and the degreasing times are 1-3. Preferably, in the step (2), the step of gelatinizing comprises the steps of mixing defatted rice bran and water, adjusting the pH to 5.5-6.5, and gelatinizing for 8-12 min at 90-100 ℃ to obtain gelatini