Search

CN-121992059-A - Preparation method and application of soybean oligopeptide capable of effectively reducing hypertension complications

CN121992059ACN 121992059 ACN121992059 ACN 121992059ACN-121992059-A

Abstract

The invention relates to the technical field of bioactive peptide processing, in particular to a preparation method and application of soybean oligopeptide capable of effectively reducing hypertension complications. The preparation method comprises the steps of crushing defatted soybean, extracting soybean protein by an alkali-dissolution acid precipitation method, adding water to 18-20 times of the weight of the soybean protein, using calcium hydroxide to raise the pH to 8.0, adding antihypertensive enzyme according to 0.5-0.8% of the soybean protein, carrying out enzymolysis for 2.0 hours, carrying out enzyme inactivation, filtration and ultrafiltration treatment to obtain an enzymolysis concentrate with MW <3kDa, adding debitterizing exopeptidase into the enzymolysis concentrate to debitterize, then adding active carbon accounting for 1.2-1.5% of the mass of the concentrate, stirring for adsorption, and freeze-drying to obtain soybean oligopeptide. The soybean oligopeptide prepared by the method not only has obvious blood pressure reducing effect in a short time, but also has lasting blood pressure reducing effect, and can effectively reduce complications such as kidney injury, blood fat rise, cardiovascular injury and the like caused by hypertension.

Inventors

  • ZHANG LUJIA

Assignees

  • 上海嘉倍潆生物科技有限公司

Dates

Publication Date
20260508
Application Date
20260312

Claims (9)

  1. 1. A method for preparing soybean oligopeptide capable of effectively reducing hypertension complications, which is characterized by comprising the following steps: (1) Extracting soybean protein, namely crushing defatted soybean, adding deionized water according to a solid-to-liquid ratio of 1:15-20, fully and uniformly mixing, regulating the pH of the solution to 8.5-9.0, continuously stirring for 1-2 h, centrifuging and collecting supernatant, regulating the pH to 4.0-4.5, centrifuging and collecting precipitate, and washing to obtain the soybean protein; (2) Preparing soybean protein zymolyte, namely adding water to 18-20 times of the weight of soybean protein, using calcium hydroxide to raise the pH to 8.0, adding antihypertensive enzyme according to 0.5-0.8% of the soybean protein, performing enzymolysis to 2.0 h, and performing enzyme inactivation, filtration and ultrafiltration treatment to obtain zymolyte concentrated solution with MW <3 kDa; (3) Debitter and fishy smell removing and drying, namely adding debitterized exopeptidase into the zymolyte concentrated solution to remove bitter, then adding active carbon accounting for 1.2-1.5% of the mass of the concentrated solution, stirring and adsorbing, and freeze drying to obtain the soybean oligopeptide.
  2. 2. The method for producing soybean oligopeptide effective in reducing complications of hypertension according to claim 1, wherein the defatted soybean is pulverized to 80 to 100 mesh in the step (1).
  3. 3. The method for preparing soybean oligopeptide capable of effectively reducing complications of hypertension according to claim 1, wherein deionized water is added in the solid-to-liquid ratio of 1:15 in the step (1) and is fully and uniformly mixed, and the temperature of continuous stirring is 45 ℃.
  4. 4. The method for preparing soybean oligopeptide capable of effectively reducing complications of hypertension according to claim 1, wherein the step (2) is characterized in that the antihypertensive enzyme is added according to 0.5% of soybean protein, the temperature is raised to 55 ℃ for enzymolysis of 2.0 h, and the pH is not adjusted in the middle.
  5. 5. The method for preparing soybean oligopeptide capable of effectively reducing complications of hypertension according to claim 1, wherein the enzyme inactivation in the step (2) comprises the steps of adjusting the pH to 5.1-5.5, adding diatomite according to 10-12% of soybean protein, and placing in a water bath at 85-90 ℃ for enzyme inactivation treatment of 20-25 min.
  6. 6. The method for preparing soybean oligopeptide capable of effectively reducing complications of hypertension according to claim 1, wherein the enzyme activity of the debittering exopeptidase in the step (3) is 75-100U/mg, and the addition amount is 0.2% of the mass of the concentrated solution.
  7. 7. The method for preparing soybean oligopeptide capable of effectively reducing complications of hypertension according to claim 1, wherein high-purity nitrogen with purity of 99.99% is introduced in the adsorption process in the step (3), the flow is controlled at 250mL/min, and the high-purity nitrogen is uniformly dispersed in liquid through an aeration head for nitrogen substitution.
  8. 8. Use of the soybean oligopeptide obtained by the preparation method of claims 1-7 for reducing complications of hypertension.
  9. 9. The use of the soy oligopeptide according to claim 8 for reducing complications of hypertension including but not limited to elevated blood lipid, kidney damage, heart damage, aortic lesions.

Description

Preparation method and application of soybean oligopeptide capable of effectively reducing hypertension complications Technical Field The invention relates to the technical field of bioactive peptide processing, in particular to a preparation method and application of soybean oligopeptide capable of effectively reducing hypertension complications. Background Hypertension is considered as a large "stealth killer" that is hazardous to human health and is presenting a continuously growing, younger, developing situation. Hypertension is an important factor for inducing pathological changes of various complications such as coronary artery lesions, electrocardiographic physiological disorders, renal dysfunction and the like, and the occurrence of hypertension is closely related to oxidative stress reaction. A large number of epidemiological studies indicate that oxidative damage is not only an important pathological basis for hypertension, but also plays a common pathological role in chronic diseases such as arthritis, metabolic syndrome, neurodegenerative diseases and the like. At present, western medicines are mainly adopted for treating hypertension, but side effects such as dry cough, headache, taste loss and hyperkalemia caused by taking chemical medicines such as lisinopril, alapril and captopril are not ignored. In contrast, food-borne drugs have characteristics of safety, mild action, and the like, and are increasingly advocated as replacement therapies for hypertension. Furthermore, various complications such as coronary artery lesions, electrocardiographic physiological disorders and renal dysfunction, which are usually accompanied by hypertension, need to be treated in combination, otherwise further damage to the patient's body is caused. Therefore, it is important to develop a food-derived substance that can not only reduce blood pressure but also effectively reduce complications of hypertension. Disclosure of Invention The invention aims to provide a preparation method of soybean oligopeptide capable of effectively reducing hypertension complications, the prepared soybean oligopeptide not only has a remarkable blood pressure reducing effect in a short time, but also has a lasting blood pressure reducing effect, and the complications such as kidney injury, blood fat rise and cardiovascular injury caused by hypertension can be effectively reduced. The aim of the invention is achieved by the following technical scheme: A preparation method of soybean oligopeptide capable of effectively reducing hypertension complications comprises the following steps: (1) Extracting soybean protein, namely crushing defatted soybean, adding deionized water according to a solid-to-liquid ratio of 1:15-20, fully and uniformly mixing, regulating the pH of the solution to 8.5-9.0, continuously stirring for 1-2 h, centrifuging and collecting supernatant, regulating the pH to 4.0-4.5, centrifuging and collecting precipitate, and washing to obtain the soybean protein; (2) Preparing soybean protein zymolyte, namely adding water to 18-20 times of the weight of soybean protein, using calcium hydroxide to raise the pH to 8.0, adding antihypertensive enzyme according to 0.5-0.8% of the soybean protein, performing enzymolysis to 2.0 h, and performing enzyme inactivation, filtration and ultrafiltration treatment to obtain zymolyte concentrated solution with MW <3 kDa; (3) Debitter and fishy smell removing and drying, namely adding debitterized exopeptidase into the zymolyte concentrated solution to remove bitter, then adding active carbon accounting for 1.2-1.5% of the mass of the concentrated solution, stirring and adsorbing, and freeze drying to obtain the soybean oligopeptide. According to a preferred embodiment of the present invention, the defatted soybeans are pulverized to 80-100 mesh in the step (1). According to a preferred embodiment of the present invention, deionized water is added in the solid-to-liquid ratio of 1:15 in the step (1), and the temperature of continuous stirring is 45 ℃. According to a preferred embodiment of the present invention, in the step (2), a antihypertensive enzyme is added according to 0.5% of the soybean protein, the temperature is raised to 55 ℃ for enzymolysis to 2.0 h, and the pH is not adjusted halfway, wherein the antihypertensive enzyme is neutral protease or alkaline protease or acid protease or enzyme preparation connected in series. According to a preferred embodiment of the present invention, the enzyme inactivation step in the step (2) is a step of adjusting pH to 5.1-5.5, adding diatomaceous earth in an amount of 10-12% of the soybean protein, and performing enzyme inactivation treatment of 20-25 min in a water bath at 85-90 ℃. According to a preferred embodiment of the present invention, the enzyme activity of the debittering exopeptidase in step (3) is 75-100U/mg, and the addition amount is 0.2% of the mass of the concentrate. According to a preferred embodiment of the present invent