CN-121992060-A - Preparation method for improving spine date seed saponin in fermentation liquor and fermentation liquor thereof

CN121992060ACN 121992060 ACN121992060 ACN 121992060ACN-121992060-A

Abstract

The invention belongs to the field of microbial fermentation, and relates to a preparation method for improving spine date seed saponin in fermentation liquor and the fermentation liquor thereof. The preparation method comprises the following steps of S1, crushing spina date seeds to obtain spina date seed powder, adding water into the spina date seed powder to prepare spina date seed powder slurry, S2, adding plant cell wall degrading enzyme into the spina date seed powder slurry, carrying out enzymolysis reaction to destroy cell structures until the reaction is complete to obtain an enzymolysis product, and S3, inoculating lactobacillus paracasei-containing microbial inoculum into the enzymolysis product to ferment to obtain fermentation liquor rich in spina date seed saponin. The method can efficiently convert and enrich the wild jujube seed saponin in the fermentation liquid by combining enzymolysis treatment with fermentation of specific microorganisms, and is simple and suitable for large-scale production. The obtained fermentation liquor is rich in spine date seed saponin and GABA, and has outstanding application potential and market value.

Inventors

FANG HAITIAN
ZHANG CHUNYANG
LIU HUIYAN
XU JUNNAN
WEI XIAOBO
DING WEI

Assignees

宁夏大学

Dates

Publication Date: 20260508
Application Date: 20260109

Claims (10)

1. The preparation method for improving the spine date seed saponin in the fermentation liquid is characterized by comprising the following steps of: s1, crushing spina date seeds to obtain spina date seed powder, wherein the spina date seed powder is added with water to prepare spina date seed powder slurry; S2, adding plant cell wall degrading enzyme into the wild jujube seed powder slurry, and performing enzymolysis reaction to destroy cell structures until the reaction is complete to obtain an enzymolysis product; And S3, inoculating a microbial inoculum containing lactobacillus paracasei (Lacticaseibacillus paracasei) into the enzymolysis product for fermentation to obtain a fermentation liquor rich in the spina date seed saponin.
2. The preparation method according to claim 1, wherein the lactobacillus paracasei is lactobacillus paracasei NXU-19004, and the inoculum size of the lactobacillus paracasei-containing microbial inoculum is 3×10 4 -3×10 6 CFU/mL.
3. The process according to claim 1, wherein the pulverizing treatment in S1 is a dry pulverizing method, and/or In S1, the ratio of the spina date seed powder to the water is 1:10 (w/v); In S2, the plant cell wall degrading enzyme is a complex enzyme consisting of pectase and cellulase.
4. A method according to any one of claims 1 to 3, wherein a complex microbial inoculum comprising lactobacillus paracasei and saccharomyces cerevisiae (Saccharomyces cerevisiae) is inoculated into the enzymatic hydrolysate.
5. The preparation method of claim 4, wherein the ratio of the number of viable bacteria of the lactobacillus paracasei to the saccharomyces cerevisiae in the composite microbial inoculum containing the lactobacillus paracasei and the saccharomyces cerevisiae is 1:1.
6. The process according to any one of claims 1 to 5, wherein the fermentation broth is rich in spiny jujube saponin or a product obtained by processing the fermentation broth is rich in spiny jujube saponin.
7. The use of the fermentation broth rich in spiny jujube kernel saponin as claimed in claim 6 or a product processed by using the fermentation broth rich in spiny jujube kernel saponin as a raw material, in any one of the following: (1) Application of semen Ziziphi Spinosae saponin in preparation of medicine; (2) Use in the preparation of a food product; (3) Application in preparing functional food; (4) The preparation method is applied to the preparation of medicines.
8. The preparation method of the spine date seed saponin is characterized by comprising the following steps: S1, taking the fermentation liquor rich in the wild jujube seed saponin as claimed in claim 6, and carrying out solid-liquid separation to obtain a liquid part; s2, extracting and purifying the spine date seed saponin in the liquid part to obtain purified spine date seed saponin; s3, processing the purified spine date seed saponin to prepare a spine date seed saponin finished product.
9. The method of claim 8, wherein the extraction and purification method comprises the steps of extracting the liquid part by an organic solvent, removing the organic solvent by rotary evaporation to obtain a crude product of the spine date seed saponin, and then carrying out adsorption and elution treatment on the crude product of the spine date seed saponin by using macroporous resin to obtain an eluent rich in spine date seed saponin, thus obtaining the purified spine date seed saponin.
10. A zizyphus jujuba saponin product obtainable by the process according to any one of claims 8 or 9.

Description

Preparation method for improving spine date seed saponin in fermentation liquor and fermentation liquor thereof Technical Field The invention belongs to the field of microbial fermentation, and particularly relates to a preparation method for improving spine date seed saponin in fermentation liquor and the fermentation liquor thereof. Background The wild jujube seed is a dry mature seed of wild jujube belonging to the Rhamnaceae, is a traditional medicinal and edible raw material, has the effects of nourishing heart and tonifying liver, calming heart and soothing nerves, arresting sweating and promoting the production of body fluid, and is widely used for symptoms such as dysphoria, insomnia, palpitation, dreaminess, deficiency and hyperhidrosis, body fluid injury, thirst and the like in the clinical of traditional Chinese medicine. Modern pharmacological studies show that the central nervous system inhibitory activities of sedation, hypnosis, anxiolytic and the like are mainly attributed to an important liposoluble component, namely the wild jujube seed saponin. At present, the method for obtaining the wild jujube saponins from the wild jujube is mainly focused on the optimization of the extraction process, such as ultrasonic-assisted extraction or microwave-assisted extraction, and aims to improve the dissolution rate and extraction efficiency of the wild jujube saponins. However, the plant cell wall serves as a natural barrier, which seriously hinders the effective access of the extraction solvent or external action to the intracellular saponin components, resulting in difficulty in sufficient extraction and limiting the final yield of the target product. In recent years, fermentation of a medicinal and edible raw material by probiotics to enhance the biological activity, generate new functional components or improve the flavor has become a research hot spot. However, most of the prior art fermentation researches on the wild jujube have focused on the improvement of total flavone and total saponin yields or the evaluation of the overall sedative activity of the fermentation products, and lack of specific fermentation processes capable of directionally enriching the wild jujube saponins. Disclosure of Invention Aiming at the technical problems, the invention provides a preparation method for improving the spiny jujube kernel saponin in fermentation liquor and the fermentation liquor thereof. The method can effectively increase the content of semen Ziziphi Spinosae saponin in the fermentation liquid, and the obtained fermentation liquid is rich in semen Ziziphi Spinosae saponin and GABA. In order to achieve the aim of the invention, the invention adopts the following technical scheme: in a first aspect, the present invention provides a method for improving the production of zizyphus jujuba saponin in a fermentation broth, the method comprising the steps of: s1, crushing spina date seeds to obtain spina date seed powder, wherein the spina date seed powder is added with water to prepare spina date seed powder slurry; S2, adding plant cell wall degrading enzyme into the wild jujube seed powder slurry, and performing enzymolysis reaction to destroy cell structures until the reaction is complete to obtain an enzymolysis product; And S3, inoculating a microbial inoculum containing lactobacillus paracasei (Lacticaseibacillus paracasei) into the enzymolysis product for fermentation to obtain a fermentation liquor rich in the spina date seed saponin. The preparation method provided by the invention comprises the steps of taking the wild jujube seed as a raw material, carrying out enzymolysis reaction by adopting plant cell wall degrading enzyme, effectively destroying the cell wall polysaccharide network structure of the wild jujube seed, promoting the sufficient dissolution of saponin components in cells, and fermenting by using lactobacillus paracasei, wherein the wild jujube seed can be enriched in the wild jujube seed saponin through adsorption and light conversion, so that the extraction rate of the wild jujube seed saponin in fermentation liquor is obviously improved. In addition, the fermentation process also promotes the generation of gamma-aminobutyric acid (GABA), and the gamma-aminobutyric acid and the spina date seed saponin have synergistic effect in tranquillizing and sleep aiding, so that the tranquillizing and sleep aiding effects can be enhanced, and the application value of the obtained fermentation liquid is improved. In the invention, the microbial agent containing lactobacillus paracasei refers to a microbial agent taking lactobacillus paracasei as a main active fermentation microorganism. The microbial inoculum aims at providing active fermentation strains, converting substrates through the metabolism of the active fermentation strains, and enriching the wild jujube saponins. The microbial inoculum can be a single microbial inoculum only containing lactobacillus paracasei, or can be a composit