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CN-121992093-A - Auxiliary reproductive development ending prediction method and electronic device

CN121992093ACN 121992093 ACN121992093 ACN 121992093ACN-121992093-A

Abstract

The application provides a prediction method for auxiliary reproductive development ending and an electronic device. The RNA markers of the application can be applied to the preparation of related products for predicting the ending of assisted reproductive development, and the RNA markers comprise one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1. The method can solve the problem that the development ending of the auxiliary reproductive embryo is difficult to effectively predict before treatment in the prior art, and is suitable for the technical field of prediction of the development ending of the reproductive embryo.

Inventors

  • ZHOU QING
  • HU CHENGBIN
  • YANG TINGYU
  • WANG WENJING
  • GAO YA
  • JIN XIN

Assignees

  • 深圳华大生命科学研究院

Dates

Publication Date
20260508
Application Date
20241107

Claims (13)

  1. Use of an RNA marker in the preparation of a related product for predicting the end of assisted reproductive development, wherein the RNA marker comprises one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  2. 2. The use according to claim 1, wherein the developmental outcomes comprise a probability of success in assisting the development of a reproductive embryo and/or a probability of success in clinical pregnancy of a reproductive embryo; Preferably, the related products comprise kits, chips and/or electronic devices.
  3. 3. A kit for predicting the end of assisted reproductive development, comprising a detection reagent for detecting the expression level of an RNA marker; the RNA markers include one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  4. 4. The kit of claim 3, further comprising probes and/or primers for detecting the RNA markers.
  5. 5. A method of predicting the end of assisted reproductive development, the method comprising: acquiring a first characteristic data set of free RNA of a seminal plasma sample of a to-be-detected object, wherein the first characteristic data set comprises expression quantity data of an RNA marker; Inputting the first characteristic data set into a prediction model of the development ending of the auxiliary reproductive embryo to obtain a prediction score, wherein the prediction score is used for indicating the success rate of the development of the auxiliary reproductive embryo of the object to be tested; the RNA markers include one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  6. 6. The method according to claim 5, wherein the seminal plasma sample of the subject is obtained before assisted reproduction by the subject.
  7. 7. The method of predicting as recited in claim 5 wherein the method of acquiring the first feature data set comprises: And quantitatively analyzing the free RNA to obtain expression quantity data of the RNA markers, thereby obtaining the first characteristic data set.
  8. 8. An electronic device for predicting the end of assisted reproductive development, the electronic device comprising: an acquisition unit for acquiring a first characteristic data set of the seminal plasma free RNA of a seminal plasma sample of an object to be detected, wherein the first characteristic data set comprises expression quantity data of an RNA marker; The prediction unit is used for inputting the first characteristic data set into a prediction model of the development ending of the auxiliary reproductive embryo, and outputting a prediction score, wherein the prediction score is used for indicating the success rate of the development of the auxiliary reproductive embryo of the object to be detected; the RNA markers include one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  9. 9. A method of screening for an RNA marker that predicts assisted reproductive outcome, the method comprising: I) Acquiring data of the expression amounts of all free RNAs in a training set sample, and acquiring a second characteristic data set, wherein the data of the expression amounts of each free RNA is used as a candidate characteristic; The training set samples comprise a seminal plasma sample with successful in-vitro embryo development and a seminal plasma sample with failed in-vitro embryo development; II) inputting each candidate feature into a formula I shown below one by one, and successively carrying out chi-square test to calculate the P value of each candidate feature; the formula I represents a formula of a predictive model of the assisted reproductive development outcome constructed by different candidate features; wherein e represents an index score; b represents the bias value of the prediction model; f 1 、f 2 、...、f n represents the value of the candidate feature in the predictive model; beta 1 、β 2 、…、β n represents a feature vector corresponding to the value of the candidate feature in the prediction model; y' represents a linear combination portion of the predictive model representing the sum of all the candidate features multiplied by all the feature vectors; III) screening the candidate characteristics of which the P value meets a preset threshold value to be used as RNA markers for predicting the ending of assisted reproductive development; the RNA markers include one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  10. 10. The screening method of claim 9, wherein the chi-square test comprises a likelihood ratio chi-square test; Preferably, the method for obtaining the expression level of the free RNA comprises the step of quantitatively analyzing the free RNA to obtain the expression level data of the RNA marker.
  11. 11. A method of constructing a predictive model of assisted reproductive development outcome, the method comprising: s1) detecting data of the expression quantity of the RNA markers in a training set sample; The training set samples comprise a seminal plasma sample with successful in-vitro embryo development and a seminal plasma sample with failed in-vitro embryo development; S2) constructing a prediction model of the development ending of the assisted reproductive embryo based on the data of the expression quantity of the RNA marker and a machine learning algorithm; the RNA markers include one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1.
  12. 12. An electronic device, the electronic device comprising: processor, and A memory for storing executable instructions of the processor; Wherein the processor is configured to perform the method of predicting the assisted reproductive development outcome of any one of claims 5 to 7, the method of screening for RNA markers of any one of claims 9 or 10, or the method of constructing the model of prediction of the assisted reproductive development outcome of claim 11 via execution of the executable instructions.
  13. 13. A computer-readable storage medium, characterized in that the computer-readable storage medium comprises a stored computer program, wherein the computer program, when run, controls a device in which the computer-readable storage medium is located to perform the method of predicting the end of assisted reproductive development according to any one of claims 5 to 7, the method of screening RNA markers of the end of assisted reproductive development according to any one of claims 9 or 10, or the method of constructing the model of prediction of the end of assisted reproductive development according to claim 11.

Description

Auxiliary reproductive development ending prediction method and electronic device Technical Field The invention relates to the technical field of prediction of reproductive embryo development ending, in particular to a prediction method and an electronic device for assisting in reproductive development ending. Background About 9% of the population of childbearing ages worldwide suffers from infertility, which will become the third greatest chronic disease following neoplastic, cardiovascular and cerebrovascular diseases, and male factors are one of the leading causes of infertility. Male sterility negatively affects the mental and quality of life of the couple's family, and therefore it is important to diagnose and treat early, finding the most suitable solution to increase the chance of fertility. Manual Assisted Reproductive Technology (ART) is a conventional means of infertility treatment, however, at present, the success rate is low, and many couples acquire embryos during the treatment cycle which are abnormal in vitro, and the failure of treatment is declared due to the inability to acquire the embryo to be implanted. Clinically, couples choose a semen delivery regimen after multiple attempts of treatment, ultimately resulting in successful pregnancy, and repeated attempts of failure place heavy physical, mental and economic stress on the patient. In the prior art, the embryo development ending is usually predicted by utilizing specific proteins in serum and follicular fluid, specific RNA in sperms and multiple groups of chemical characteristics in embryo culture fluid, and when the embryo development ending is predicted in the prior art, a patient enters a treatment period, and has defects in a prediction time window, so that corresponding physical, mental and economic burdens are generated on the patient. Thus, there remains a lack of solutions in the art that can effectively predict the end of assisted reproductive embryo development prior to treatment. Disclosure of Invention The invention mainly aims to provide a method for predicting the assisted reproductive development ending and an electronic device, so as to solve the problem that the effective prediction of the assisted reproductive embryo development ending is difficult to carry out before treatment in the prior art. To achieve the above object, according to a first aspect of the present invention, there is provided the use of an RNA marker comprising one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1 for the preparation of a related product for predicting the end of assisted reproductive development. Further, the developmental outcomes include a probability of success in assisting the development of the reproductive embryo and/or a probability of success in clinical pregnancy of the reproductive embryo, preferably the related products include a kit, chip and/or electronic device. In order to achieve the above object, according to a second aspect of the present invention, there is provided a kit for predicting the end of assisted reproductive development, the kit comprising a detection reagent for detecting the expression level of an RNA marker, wherein the RNA marker comprises one or more of the following RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1. Further, the kit comprises probes and/or primers for detecting RNA markers. According to a third aspect of the invention, a method for predicting the end of assisted reproductive development is provided, which comprises the steps of obtaining a first characteristic dataset of free RNA of a seminal plasma sample of a subject to be tested, wherein the first characteristic dataset comprises expression level data of an RNA marker, inputting the first characteristic dataset into a prediction model of the end of assisted reproductive embryo development to obtain a prediction score, wherein the prediction score is used for indicating the success rate of the assisted reproductive embryo development of the subject to be tested, and the RNA marker comprises one or more of RNA markers ：NECTIN2、TUBB3、SAXO1、FBLN2、CLIP2、BCL2L15、ASB12、CRYAB、PLAAT4、ENSG00000258422、ENSG00000233633、ENSG00000247311 or LURAP L-AS1. Further, a seminal plasma sample of the object to be measured is obtained before assisted reproduction of the object to be measured. Further, the method for acquiring the first characteristic data set comprises the step of quantitatively analyzing the free RNA to acquire the expression quantity data of the RNA markers, thereby acquiring the first characteristic data set. According to a fourth aspect of the present invention, there is provided an electronic device for predicting an end of assisted reproductive development, the electronic device comprising an acquisition unit for acquiring a first featur