CN-121992112-A - SNP molecular marker for identifying female sturgeon oviposition phenotype and application thereof

Abstract

The invention discloses a SNP molecular marker for identifying female sturgeon egg mass phenotype and application thereof, wherein the SNP molecular marker is positioned at 24495851 bp of sturgeon NC-081195.1 chromosome, namely 258 bp of sturgeon chromosome 7 nucleotide fragment (SEQ ID NO: 1), the polymorphism form is T/G base, and the genotype of a molecular marker locus comprises TT, GG or TG. The SNP molecular marker provided by the invention can be used for early artificial screening of female sturgeon egg mass phenotype, and can be used for screening in multiple female sturgeon populations such as Acipenser sinensis, huso dauricus, siberian sturgeon, small sturgeon, hybrid sturgeon and the like. The SNP molecular marker lays a foundation for marker-assisted selection of female sturgeon individuals with high-bosom-content phenotypes, so that the artificial breeding process of high-bosom-content sturgeon varieties is remarkably accelerated.

Inventors

• LI YUTAO
• WANG RUOYU
• ZHANG YING
• MA BO

Assignees

• 中国水产科学研究院黑龙江水产研究所

Dates

Publication Date

20260508

Application Date

20260225

Claims (3)

1. 1. A SNP molecular marker for identifying female sturgeon's fecundity phenotype is characterized in that the SNP molecular marker is positioned at 258 bp of a sturgeon chromosome 7 nucleotide fragment SEQ ID NO. 1, the polymorphism form is T/G base, the genotype of a molecular marker locus comprises TT, GG or TG, the primer sequence for detecting the genotype of the molecular marker locus is that a forward primer is shown as SEQ ID NO. 2, and a reverse primer is shown as SEQ ID NO. 3.
2. 2. Use of the SNP molecular marker of claim 1 for identifying female sturgeon oviposition phenotypes.
3. 3. Use of the SNP molecular markers according to claim 2 for identifying female sturgeon egg mass phenotypes, characterized in that said step of identifying female sturgeon egg mass phenotypes comprises the following steps: step one, extracting genome DNA from sturgeon tail fin tissues to be detected; Step two, performing PCR amplification on the genomic DNA of the sturgeon to be detected by using the detection primers shown in SEQ ID NO. 2 and SEQ ID NO. 3 to obtain a nucleotide sequence fragment with the length of 528 bp in a sturgeon chromosome 7, namely SEQ ID NO. 1; Detecting the genotype of the SNP molecular marker according to claim 1, wherein female individuals with genotypes TT and GG belong to individuals with low egg mass, and female individuals with genotype TG belong to individuals with high egg mass.

Description

SNP molecular marker for identifying female sturgeon oviposition phenotype and application thereof Technical Field The invention belongs to the field of sturgeon molecular marker-assisted selective breeding, and relates to a female sturgeon broodstock scale-related SNP molecular marker and application thereof in artificially screening individuals with high broodstock. Background The major economic value of sturgeons is the unfertilized eggs produced during sexual maturity in their female individuals, i.e., the favored "caviar". Caviar is favored as "black gold in water" because of its high nutritional, medicinal, and delicious taste. Therefore, early screening of female individuals with high egg numbers is of great importance for the development of the caviar industry with high quality. With the advent of the "post-genome" age, molecular markers have been widely used for early artificial screening of animal strain identification and superior economic traits. In recent years, due to the increasing sophistication of second generation sequencing technologies and genome public databases, whole genome re-sequencing (Whole-genome resequencing) technology has become a powerful tool for developing potential molecular markers. As a third generation genetic marker, a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site refers to a single base variation site generated by an organism at the genome level and is unevenly distributed over the whole genome. SNP molecular markers are widely applied in the field of aquatic animal marker-assisted selection (MAS) breeding. Therefore, the development of SNP molecular markers related to excellent economic characters has important significance for shortening the generation interval, realizing early artificial screening of individuals with excellent characters and accelerating the breeding process of new varieties. Heretofore, the existence of SNP molecular markers related to the oviposition scale in the 7 th chromosome (NC_ 081195.1) of sturgeon has not been described in the prior art. Disclosure of Invention In order to facilitate identification of female sturgeon egg mass phenotype, the invention provides an SNP molecular marker for identifying female sturgeon egg mass phenotype and application thereof. The molecular marker can be used for identifying the female sturgeon egg-scale type, wherein the SNP locus has obvious correlation with the female sturgeon egg-scale type, so that early artificial screening of individuals with high egg-scale type can be realized by detecting the genotype of female sturgeon individuals at the locus. The invention aims at realizing the following technical scheme: SNP molecular marker for identifying female sturgeon egg mass phenotype, which is located at 24495851 bp of sturgeon chromosome 7 (NC_ 081195.1), namely 258 bp of sturgeon chromosome 7 nucleotide fragment (SEQ ID NO: 1), wherein the polymorphism form is T/G base, the genotype of the molecular marker locus comprises TT, GG or TG, and the primer sequence for detecting the genotype of the molecular marker locus is: Forward primer F5'-GAGGAGTGCCCAAGCCTTGT-3', SEQ ID NO. 2; The reverse primer R5'-CCTCTGCAAGGGATGCCAC-3' is SEQ ID NO. 3. An application of the SNP molecular marker in identifying female sturgeon egg mass phenotype, comprising the following steps: step one, extracting genome DNA from sturgeon tail fin tissues to be detected; Step two, performing PCR amplification on sturgeon genomic DNA to be detected by using the detection primers shown in SEQ ID NO. 2 and SEQ ID NO. 3 to obtain a nucleotide sequence fragment with the length of 528 bp in a sturgeon chromosome 7 (NC_ 081195.1), namely SEQ ID NO. 1; And thirdly, detecting the genotype of the 258 bp th site (namely the target molecular marker locus) of the PCR amplification product to identify the egg mass phenotype of the female sturgeon individuals, wherein female individuals with genotypes TT and GG belong to individuals with low egg mass (egg weight/body weight < 0.15), and female individuals with genotypes TG belong to individuals with high egg mass (egg weight/body weight > 0.25). Compared with the prior art, the invention has the following advantages: 1. The SNP molecular marker provided by the invention is positioned at 24495851 bp of sturgeon NC-081195.1 chromosome, namely 258 bp of sturgeon chromosome 7 nucleotide fragment (SEQ ID NO: 1). The molecular marker has T/G base mutation and has obvious correlation with female sturgeon egg-carrying scale, wherein the egg-carrying amount of TT and GG genotype individuals is obviously lower than that of TG genotype, and meanwhile, the matching rate of the genotyping result of the SNP molecular marker locus and the actual egg-carrying amount phenotype of female individuals can reach 83.33%. 2. The SNP molecular marker provided by the invention can be used for early artificial screening of female sturgeon egg mass phenotype, and can be used for screening in multip