CN-121992117-A - SNP molecular marker associated with fullness under turbot fermented soybean meal feeding and application thereof

CN121992117ACN 121992117 ACN121992117 ACN 121992117ACN-121992117-A

Abstract

The invention relates to a SNP molecular marker associated with the fullness of turbot fermented soybean meal fed and application thereof, belonging to the technical field of molecular markers, the invention utilizes whole genome association analysis to analyze the fullness character of turbot to obtain the SNP molecular marker obviously associated with the fullness character, the method comprises at least one of 6 SNP loci, can be applied to turbot molecular assisted breeding, can greatly shorten the breeding period, reduce the dependence on phenotype characteristics, improve the selective breeding efficiency and have good application prospect by screening on a genome level by the breeding method disclosed by the invention.

Inventors

YANG JINGKUN
MA AIJUN
WANG XINAN
SUN ZHIBIN
LIU ZHIFENG
HUANG ZHIHUI
GAO YUNYI
WEN HAISHEN

Assignees

中国海洋大学
中国水产科学研究院黄海水产研究所

Dates

Publication Date: 20260508
Application Date: 20260408

Claims (6)

1. A SNP molecular marker associated with the fullness of turbot fermented soybean meal fed is characterized in that the molecular marker is at least one of the following, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the 101 st position of the molecular marker is provided with a SNP locus associated with the fullness of turbot, the allele of the locus is A or G, and the molecular marker is marked as chr11:6173666; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is A or T, and the molecular marker is marked as chr11:6752389; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is T or A, and the molecular marker is marked as chr11:6557587; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, and the allele of the locus is T or C, and the molecular marker is marked as chr11:6163141; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with an SNP locus related to turbot fertility, and the allele of the locus is T or C, and the molecular marker is marked as chr11:6193220; the nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is T or C, and the molecular marker is marked as chr11:6730072.
2. A set of brill fermented soybean meal fed with a combination of related fullness SNP molecular markers, characterized in that the combination of SNP molecular markers is one of chr11:6173666 and chr11:675389, chr11:6173666 and chr11:6193220, chr11:6173666, chr11:6752389 and chr11:6557587, hr11:6173666, chr11:6752389, chr11:6557587 and chr11:6163141, chr11:675389, chr11:6557587, chr11:6163141 and chr11:6193220, chr11:6173666, chr11:6752389, chr11:6557587, chr11:6163141 and chr11:6193220, chr11:6173666, chr11:6752389, chr11:6557587, chr11:6163141, chr11:6193220 and chr11:6730072, the molecular markers being as defined in claim 1.
3. The primer for amplifying the SNP molecular marker of claim 1, wherein the primer is capable of amplifying the SNP molecular marker.
4. The use of the SNP molecular marker according to claim 1 in turbot fertility trait breeding, characterized in that the use is by selecting dominant genotype individuals in the corresponding molecular marker as fertility trait breeding parents.
5. Use of the molecular marker combination according to claim 2 in turbot fertility trait breeding by selecting individuals with the dominant genotype in the molecular marker combination as a fertility trait breeding parent.
6. The application of the primer in turbot fertility trait breeding, which is characterized in that the primer is used for amplifying turbot individuals and screening individuals with dominant genotypes in SNP molecular markers as defined in claim 1 as fertility trait breeding parents.

Description

SNP molecular marker associated with fullness under turbot fermented soybean meal feeding and application thereof Technical Field The invention belongs to the technical field of molecular markers, and particularly relates to a SNP molecular marker associated with the fullness of turbot fermented soybean meal fed and application thereof. Background Turbot (Scophthalmus maximus) is an important economic fish for cold water mariculture in northern China, and has high commercial value due to high growth speed and delicious meat quality. In the traditional cultivation mode, the growth of turbots is highly dependent on ration with high fish meal content. However, with the shortage of global fish meal resources and rising prices, the development of efficient, low cost vegetable protein sources (e.g., soybean meal) to replace fish meal has become a necessary trend in the aquaculture feed industry. Research shows that the anti-nutritional factors in the common soybean meal can induce turbots to have physiological problems such as intestinal inflammation, reduced digestibility and the like. Although the fermented soybean meal remarkably reduces the content of anti-nutritional factors and improves the palatability through microbial fermentation, in actual production, turbot individuals with different genetic backgrounds are found to have remarkable differences in the utilization capacity of the fermented soybean meal daily ration. This genotype interaction with nutrient metabolism results in significant differentiation of individual growth in the population under low fish meal (high fermented soybean meal) feeding conditions. The traditional breeding method mainly depends on phenotype selection, is greatly influenced by environment, has long period, and is difficult to accurately screen out good varieties with high-efficiency transformation capability on novel feeds. Although turbot growth trait-associated SNPs (single nucleotide polymorphisms) have been reported in recent years, the contribution rate of genetic variation explained by single SNP sites is generally limited. Haplotypes (Haplotype) consist of a set of SNP sites that are closely linked in physical position, and compared with single SNP, haplotypes can provide more abundant genetic information, and have higher polymorphism and genetic stability. Under the background of fermented soybean meal feeding, haplotypes obviously related to the fertility traits are excavated and applied to early molecular marker assisted breeding (MAS), and the method has important economic value and scientific research significance for cultivating novel turbot varieties with excellent plant protein fish meal feed tolerance and growth traits. Disclosure of Invention Aiming at the technical problems, the invention provides a group of SNP molecular markers in the fermented soybean meal feeding background, which can be used for the molecular auxiliary breeding of new lines of turbot fertility traits. The invention aims to provide a SNP molecular marker associated with the fullness of turbot fermented soybean meal fed, wherein the molecular marker is at least one of the following nucleotide sequences shown in SEQ ID NO.1, the 101 st position of the molecular marker is provided with a SNP locus associated with the fullness of turbot, the allele of the locus is A or G, and the molecular marker is denoted as chr11:6173666; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is A or T, and the molecular marker is marked as chr11:6752389; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is T or A, and the molecular marker is marked as chr11:6557587; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with an SNP locus related to turbot fertility, and the allele of the locus is T or C, and the molecular marker is marked as chr11:6163141; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with an SNP locus related to turbot fertility, and the allele of the locus is T or C, and the molecular marker is marked as chr11:6193220; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, the 101 st position of the molecular marker is provided with a SNP locus related to turbot fertility, the allele of the locus is T or C, and the molecular marker is denoted as chr11:6730072. The second object of the invention is to provide a SNP molecular marker combination which is related to the fullness of turbot fermented soybean meal fed, wherein the SNP molecular marker combination is one of chr11:6173666 and chr11:675389, chr11:6173666 and