CN-121992118-A - SNP molecular marker associated with lower body length of turbot fermented soybean meal feeding and application thereof

CN121992118ACN 121992118 ACN121992118 ACN 121992118ACN-121992118-A

Abstract

The invention relates to a SNP molecular marker related to the body length of turbot under the feeding of fermented soybean meal and application thereof, belonging to the technical field of molecular markers, the method comprises at least one of 6 SNP loci, can be applied to turbot molecular assisted breeding, can greatly shorten the breeding period, reduce the dependence on phenotype characteristics, improve the selective breeding efficiency and have good application prospect by screening on a genome level by the breeding method disclosed by the invention.

Inventors

MA AIJUN
SUN ZHIBIN
YANG JINGKUN
WANG XINAN
LIU ZHIFENG
HUANG ZHIHUI
GAO YUNYI
WANG YILIN
MA CONG

Assignees

中国水产科学研究院黄海水产研究所

Dates

Publication Date: 20260508
Application Date: 20260408

Claims (6)

1. A SNP molecular marker associated with the length of a turbot fermented soybean meal fed lower body is characterized in that the molecular marker is at least one of the following nucleotide sequences of the molecular marker are shown as SEQ ID NO.1, and the 101 st position of the molecular marker is provided with a SNP locus associated with the length of the turbot body, and the allele of the locus is G or A; the nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is A or T; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is T or A; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is C or T; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is T or C; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is T or A.
2. A set of SNP molecular marker combinations associated with the lower body length of turbot fermented soybean meal fed, characterized in that the SNP molecular marker combinations are one of chr7:4184141 and chr12:871680, chr7:418441, chr12:871680 and chr15:1901316, chr7:1293734, chr12:871680 and chr15:1783670, chr7:418441, chr7:1293734, chr12:871680 and chr15:1316, chr7:418441, chr12:24383879, chr15:1901316 and chr12:871680, chr7:418441, chr7:1293734, chr12:871680, chr12:24383879 and chr15:1901316, chr7:418441, chr7:1293734, chr12:871680, chr12:24383879, chr15:1901316 and chr15:1783670, and the SNP markers as defined in claim 1.
3. The primer for amplifying the SNP molecular marker of claim 1, wherein the primer is capable of amplifying the SNP molecular marker.
4. The use of the SNP molecular marker according to claim 1 in turbot body length trait breeding, characterized in that the use is by selecting individuals with dominant genotypes as body length trait breeding parents.
5. Use of a combination of molecular markers according to claim 2 in turbot somatization, characterized in that the use is by selecting individuals with a dominant genotype combination as somatization parents.
6. The application of the primer in turbot body length trait breeding, which is characterized in that the primer is used for amplifying turbot individuals and screening individuals with dominant genotypes in SNP molecular markers as set forth in claim 1 as body length trait breeding parents.

Description

SNP molecular marker associated with lower body length of turbot fermented soybean meal feeding and application thereof Technical Field The invention belongs to the technical field of molecular markers, and particularly relates to a SNP molecular marker associated with the length of a turbot fermented soybean meal fed lower body and application thereof. Background Turbot (Scophthalmus maximus) is an important economic fish for cold water mariculture in northern China, and has high commercial value due to high growth speed and delicious meat quality. In the traditional cultivation mode, the growth of turbots is highly dependent on ration with high fish meal content. However, with the shortage of global fish meal resources and rising prices, the development of efficient, low cost vegetable protein sources (e.g., soybean meal) to replace fish meal has become a necessary trend in the aquaculture feed industry. Research shows that the anti-nutritional factors in the common soybean meal can induce turbots to have physiological problems such as intestinal inflammation, reduced digestibility and the like. Although the fermented soybean meal remarkably reduces the content of anti-nutritional factors and improves the palatability through microbial fermentation, in actual production, turbot individuals with different genetic backgrounds are found to have remarkable differences in the utilization capacity of the fermented soybean meal daily ration. This genotype interaction with nutrient metabolism results in significant differentiation of individual growth in the population under low fish meal (high fermented soybean meal) feeding conditions. The traditional breeding method mainly depends on phenotype selection, is greatly influenced by environment, has long period, and is difficult to accurately screen out good varieties with high-efficiency transformation capability on novel feeds. Although turbot growth trait-associated SNPs (single nucleotide polymorphisms) have been reported in recent years, the contribution rate of genetic variation explained by single SNP sites is generally limited. Haplotypes (Haplotype) consist of a set of SNP sites that are closely linked in physical position, and compared with single SNP, haplotypes can provide more abundant genetic information, and have higher polymorphism and genetic stability. Under the background of fermented soybean meal feeding, haplotypes obviously related to the body length characters are excavated and applied to early molecular marker assisted breeding (MAS), and the method has important economic value and scientific research significance for cultivating novel turbot varieties with excellent plant protein fish meal feed tolerance and growth characters. Disclosure of Invention Aiming at the technical problems, the invention provides a group of SNP molecular markers in the fermented soybean meal feeding background, which can be used for the molecular auxiliary breeding of new lines with turbot body length characters. The invention aims to provide a SNP molecular marker associated with the length of a turbot fermented soybean meal fed lower body, wherein the molecular marker is at least one of the following nucleotide sequences shown in SEQ ID NO.1, the 101 st position of the molecular marker is provided with a SNP locus associated with the length of the turbot body, the allele of the locus is G or A, and the molecular marker is denoted as chr7:4184141; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, the allele of the locus is A or T, and the molecular marker is marked as chr7:1293734; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, the allele of the locus is T or A, and the molecular marker is marked as chr12:871680; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is C or T; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, and the allele of the locus is T or C; the nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body length, the allele of the locus is T or A, and the molecular marker is marked as chr15:1783670. It is a second object of the present invention to provide a set of SNP molecular marker combinations associated with turbot fermented soybean meal fed lower body length, said SNP molecular marker combinations being one of the following: chr7:4184141 and chr12:871680, chr7:418441, chr12:871680 and chr15:1901