CN-121992135-A - Corn ZmNF-YC3 gene and SNP molecular marker and application thereof

Abstract

The invention relates to a corn ZmNF-YC3 gene, and SNP molecular markers and application thereof, and relates to the technical field of plant genetic engineering, wherein the nucleotide sequence of the corn ZmNF-YC3 gene is shown as SEQ ID NO.1, the amino acid of a coded protein is shown as SEQ ID NO.2, and the corn ZmNF-YC3 gene positively regulates and controls the plant height and the ear position height of a corn plant. According to the invention, through analyzing the expression profile of the corn ZmNF-YC3 gene, carrying out plant height character candidate gene association analysis on the corn ZmNF-YC3 gene, and carrying out plant height and spike position Gao Biaoxing analysis on the ZmNF-YC3 gene knockout corn mutant material, the positive regulation and control effect of the corn ZmNF-YC3 gene on the corn plant height and spike position character is proved, and important gene resources and molecular markers are provided for creating new dwarf and dense-tolerant corn germplasm by utilizing a molecular breeding means.

Inventors

• HUANG XINER
• WANG XI
• ZHU QIAOYUN
• Mao ziwei
• ZHANG YAN
• JIANG HAIYANG

Assignees

• 安徽农业大学

Dates

Publication Date

20260508

Application Date

20260210

Claims (8)

1. 1. The corn ZmNF-YC3 gene is characterized in that the nucleotide sequence of the corn ZmNF-YC3 gene is shown as SEQ ID NO. 1.
2. 2. A protein encoded by the ZmNF-YC3 gene of maize according to claim 1, wherein the amino acid of said protein encoded is shown in SEQ ID No. 2.
3. 3. Use of the maize ZmNF-YC3 gene of claim 1 for regulating plant height and ear position height.
4. 4. The use according to claim 3, wherein the maize ZmNF-YC3 gene positively regulates maize plant height and ear height.
5. 5. The use according to claim 4, wherein the loss of gene function of the maize ZmNF-YC3 gene reduces both plant height and ear height of maize plants.
6. 6. The use according to claim 5, characterized in that the maize is maize inbred line KN5585.
7. 7. The method for obtaining corn germplasm with low plant height and/or low spike height is characterized in that corn ZmNF-YC3 genes in corn according to any one of claims 1-6 are edited by CRISPR-Cas9 gene editing technology, expression of the corn ZmNF-YC3 genes is inhibited, corn genetic transformation is carried out after a ZmNF-YC3-CRISPR-Cas9 gene editing vector is constructed, and obtained positive seedlings are corn germplasm with low plant height and/or low spike height.
8. 8. The SNP molecular marker for identifying the plant height and the spike height of the corn plant based on the ZmNF-YC3 gene is characterized in that the nucleotide sequence of the SNP molecular marker comprises 837bp base and upstream and downstream bases thereof and 1649bp base and upstream and downstream bases thereof which are respectively positioned at the downstream of a termination codon of the ZmNF-YC3 gene as shown in SEQ ID NO. 3; Wherein the mutation type of the 837bp base downstream of the ZmNF-YC3 gene stop codon is C or A, and the mutation type of the 1649bp base downstream of the ZmNF-YC3 gene stop codon is C or T; When the genotypes of the two SNP molecular marker loci of the corn to be detected are CC, the corn to be detected with low plant height and low spike height is obtained.

Description

Corn ZmNF-YC3 gene and SNP molecular marker and application thereof Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to a corn ZmNF-YC3 gene, and SNP molecular markers and application thereof. Background Corn (Zea mays L.) occupies a significant position in an industrial production and agricultural system, the high yield and stable yield of the corn have important influence, the plant height is an important agronomic property in the corn, the lodging resistance of the plant is influenced, the photosynthetic efficiency and nutrient absorption are also influenced, the final yield is further influenced, the risk of lodging of the corn plant is easily increased due to the excessively high plant height, the high yield is difficult to obtain, the light energy capturing efficiency is reduced due to the excessively low plant height, the energy supply is influenced, the yield is similarly restricted, and therefore, functional genes for controlling the high property of the corn plant are mined, the molecular mechanism of the high property of the corn plant is facilitated to be analyzed, and excellent gene resources and application basis are provided for stress-tolerant breeding of the corn. More and more researches show that NF-YC family transcription factors are widely involved in the regulation of plant growth and development, biological and abiotic stress responses and other biological processes, more functional reports exist on genes of the type in arabidopsis and rice, such as responses to adversity stress such as embryo and seed development, drought and the like and important biological processes such as flowering and the like, and researches on ZmNF-YC family genes in regulation of maize plant type development are relatively less, especially in regulation of maize plant height, so that deep excavation of ZmNF-YC family genes in regulation of maize plant height has important significance. Disclosure of Invention The invention aims to solve the problems and provide a corn ZmNF-YC3 gene, SNP molecular markers and application thereof. The invention realizes the above purpose through the following technical scheme: the invention provides a corn ZmNF-YC3 gene, and the nucleotide sequence of the corn ZmNF-YC3 gene is shown as SEQ ID NO. 1. The invention also provides a coding protein of the corn ZmNF-YC3 gene, and the amino acid of the coding protein is shown as SEQ ID NO. 2. The invention also provides application of the corn ZmNF-YC3 gene in regulating plant height and spike position height. As a further optimization scheme of the invention, the corn ZmNF-YC3 gene positively regulates the plant height and the ear position height of a corn plant. As a further optimization scheme of the invention, the corn ZmNF-YC3 gene has the gene function deletion, so that the plant height and the ear position height of corn plants are reduced. As a further optimization of the invention, the corn is a corn inbred line KN5585. A method for obtaining corn germplasm with low plant height and/or low spike height comprises the steps of editing corn ZmNF-YC3 genes in corn through a CRISPR-Cas9 gene editing technology, inhibiting the expression of the corn ZmNF-YC3 genes, constructing a ZmNF-YC3-CRISPR-Cas9 gene editing vector, and then carrying out corn genetic transformation, wherein positive seedlings obtained are corn germplasm with low plant height and/or low spike height. SNP molecular markers for identifying the plant height and the ear height of corn plants based on ZmNF-YC3 genes, wherein the nucleotide sequence of the SNP molecular markers comprises 837bp base and upstream and downstream base as well as 1649bp base and upstream and downstream base respectively positioned at the downstream of a ZmNF-YC3 gene stop codon as shown in SEQ ID NO. 3; Wherein the mutation type of the 837bp base downstream of the ZmNF-YC3 gene stop codon is C or A, and the mutation type of the 1649bp base downstream of the ZmNF-YC3 gene stop codon is C or T; When the genotypes of the two SNP molecular marker loci of the corn to be detected are CC, the corn to be detected with low plant height and low spike height is obtained. The invention has the beneficial effects that: According to the invention, through analyzing the expression profile of the corn ZmNF-YC3 gene, carrying out plant height character candidate gene association analysis on the corn ZmNF-YC3 gene, and carrying out plant height and spike position Gao Biaoxing analysis on the ZmNF-YC3 gene knockout corn mutant material, the positive regulation and control effect of the corn ZmNF-YC3 gene on the corn plant height character is proved, and important gene resources and molecular markers are provided for creating new dwarf and dense-tolerant corn germplasm by utilizing a molecular breeding means. Drawings FIG. 1 is a maize NF-YC family protein evolutionary tree; FIG. 2 is a statistical chart of the expression level of ZmNF-YC3 g