CN-121994557-A - Integrated desalting device and method for high-flux sample treatment

CN121994557ACN 121994557 ACN121994557 ACN 121994557ACN-121994557-A

Abstract

The application relates to the technical field of experimental equipment, and provides an integrated desalting device and method for high-flux sample treatment. The movable partition has first and second working positions. The wedge-shaped guiding and positioning mechanism is arranged between the movable partition board and the porous board, and can automatically guide the movable partition board to lift and transversely align when the porous board is pushed in horizontally through the inclined plane fit until the movable partition board is stably supported on the porous board and the accurate alignment of the sample processing column and the collecting hole is realized, so that the rapid and accurate switching between the washing and eluting modes is completed. The application has smart structure and simple and smooth operation.

Inventors

ZHU XUDONG
YIN DONG

Assignees

中山大学孙逸仙纪念医院

Dates

Publication Date: 20260508
Application Date: 20251231

Claims (8)

1. An integrated desalination device for high throughput sample processing, comprising: a base provided with a plurality of first accommodation parts distributed in an array; a movable partition plate arranged above the base, provided with through holes corresponding to the first accommodation parts one by one, for bearing sample processing columns, and A standard multi-well plate; the movable partition plate is provided with a first working position and a second working position; When the movable partition board is positioned at the first working position, the movable partition board is positioned on the base, and the outlet at the lower end of the sample processing column is opposite to the corresponding first accommodating part; when in the second working position, the standard porous plate is arranged between the base and the movable partition plate, and the outlet at the lower end of the sample processing column is opposite to the corresponding hole of the standard porous plate; The movable partition board and the standard porous board are provided with wedge-shaped guide positioning mechanisms which are matched with each other, and the wedge-shaped guide positioning mechanisms are configured to enable the movable partition board to lift and transversely align relative to the standard porous board through inclined surface guide contact in the process of horizontally pushing the standard porous board into the space between the base and the movable partition board until the movable partition board and the standard porous board reach a preset relative position, the movable partition board is stably supported on the standard porous board, accurate alignment of the through holes and corresponding holes of the standard porous board is achieved, and switching to the second working position is completed.
2. An integrated desalination device for high throughput sample processing according to claim 1 wherein said wedge-shaped guiding and positioning mechanism comprises: First wedge-shaped guide parts arranged on two sides of the lower surface of the movable partition board and The second wedge-shaped guide parts are arranged on two sides of the upper surface of the standard porous plate and matched with the first wedge-shaped guide parts; The first wedge-shaped guide part and the second wedge-shaped guide part are complementary in cross section shape in the horizontal direction, and when the first wedge-shaped guide part and the second wedge-shaped guide part are completely folded, a rectangular positioning part is formed together, and at the moment, the movable partition plate reaches the second working position.
3. An integrated desalination device for high throughput sample treatment according to claim 2 wherein the first wedge shaped guide has a lower slope and the second wedge shaped guide has an upper slope.
4. An integrated desalination device for high throughput sample treatment according to claim 3 wherein the wedge-shaped guiding and positioning mechanism further comprises an anti-disengaging structure, the anti-disengaging structure being a T-shaped or dovetail-shaped rail disposed on the lower slope of the first wedge-shaped guiding portion and a chute matching the rail disposed on the upper slope of the second wedge-shaped guiding portion.
5. An integrated desalination device for high throughput sample processing according to claim 1 wherein the lower surface of the movable partition is further provided with an annular locating flange surrounding the through hole, the lower end surface of the annular locating flange being in press contact with the upper surface of the standard porous plate when in the second working position.
6. An integrated desalination device for high throughput sample processing according to claim 1, further comprising a cover plate that can be placed over the movable partition carrying the sample processing column, the cover plate being provided with openings distributed in an array and interfaces for connecting negative or positive pressure devices.
7. A method of high throughput sample processing, characterized in that the method is carried out based on an integrated desalination device for high throughput sample processing according to any one of claims 1-6, the method comprising the steps of: S1, placing the movable partition board carrying a plurality of sample treatment columns on the base, enabling the movable partition board to be in a first working position, performing sample treatment operation, and collecting waste liquid in the first accommodating part; S2, horizontally pushing the standard porous plate between the base and the movable partition plate from the side of the device, wherein in the pushing process, the movable partition plate is laterally limited by virtue of the guidance of the wedge-shaped guiding and positioning mechanism and is automatically lifted and horizontally aligned under the pushing of the standard porous plate until the standard porous plate is stably supported on the standard porous plate and reaches a second working position; S3, in the second working position, eluting, and collecting the target components directly into the corresponding holes of the standard porous plate.
8. The method of claim 7, wherein in step S2, the pushing operation is a single direction linear motion without manually adjusting the movable partition in a vertical direction or other directions, and the wedge-shaped guiding and positioning mechanism automatically performs a combined motion of height lifting and lateral alignment.

Description

Integrated desalting device and method for high-flux sample treatment Technical Field The invention belongs to the technical field of experimental equipment, and particularly relates to an integrated desalting device and method for high-flux sample treatment. Background In life science research such as proteomics, metabonomics and the like, desalting or desalting purification of biological samples (such as peptide fragments after enzymolysis) is a vital pretreatment step before mass spectrometry. Currently, high throughput desalination is mainly performed in a 96-well plate format. The prior art schemes are divided into two types, namely, a chromatography column or a centrifugal column is improved, and the chromatography column or the centrifugal column is integrated into a row shape (such as CN 209166966U), so that the automatic grabbing is facilitated, but the flexibility is poor, the cost is high, and the single centrifugal column is incompatible with a single centrifugal column commonly used in a laboratory. Another type is to design a carrier device (e.g., CN 215296882U), and assemble and align the general micro-chromatography column, collection tube, and spot plate with a multi-layered support structure. The scheme has good compatibility, but has obvious defects that firstly, additional parts such as a fixed support and a support column are required, the assembly and the disassembly are complicated, and secondly, when two key steps of washing (collecting waste liquid) and eluting (collecting samples) are carried out, a collection container at the bottom (such as a collection pipe is replaced by a spot plate) is required to be manually replaced or adjusted, so that the operation steps and the pollution risk of the samples are increased, and the operation process is not visual and smooth. In addition, there is a general chromatographic collection device (such as CN 211905242U), which can switch collection tubes by sliding, but it cannot realize seamless and rapid switching between washing and eluting modes, and it is difficult to directly adapt to standardized spot plates. Therefore, there is a strong need in the art for a high throughput sample processing device that has a simpler structure, higher integration, seamless connection of different processing stages (e.g., waste liquid collection and sample elution collection) on the same device, and perfect compatibility with laboratory consumables (standard 96-well plates, various specifications of micro-chromatography columns). Disclosure of Invention The invention aims to overcome the defects of the prior art and provides the integrated desalting device and the method for high-flux sample treatment, which have the advantages of simple structure, visual operation and capability of realizing rapid switching of washing and eluting modes. In order to achieve the above purpose, the present invention provides the following technical solutions: In a first aspect, the present invention provides an integrated desalination device for high throughput sample processing, comprising: a base provided with a plurality of first accommodation parts distributed in an array; a movable partition plate arranged above the base, provided with through holes corresponding to the first accommodation parts one by one, for bearing sample processing columns, and A standard multi-well plate; the movable partition plate is provided with a first working position and a second working position; When the movable partition board is positioned at the first working position, the movable partition board is positioned on the base, and the outlet at the lower end of the sample processing column is opposite to the corresponding first accommodating part; when in the second working position, the standard porous plate is arranged between the base and the movable partition plate, and the outlet at the lower end of the sample processing column is opposite to the corresponding hole of the standard porous plate; The movable partition board and the standard porous board are provided with wedge-shaped guide positioning mechanisms which are matched with each other, and the wedge-shaped guide positioning mechanisms are configured to enable the movable partition board to lift and transversely align relative to the standard porous board through inclined surface guide contact in the process of horizontally pushing the standard porous board into the space between the base and the movable partition board until the movable partition board and the standard porous board reach a preset relative position, the movable partition board is stably supported on the standard porous board, accurate alignment of the through holes and corresponding holes of the standard porous board is achieved, and switching to the second working position is completed. Further, the wedge-shaped guiding and positioning mechanism comprises: First wedge-shaped guide parts arranged on two sides of the lower surface of the movable partition board