CN-121994575-A - Paraffin embedding device for pathological section and control method thereof

CN121994575ACN 121994575 ACN121994575 ACN 121994575ACN-121994575-A

Abstract

The invention relates to the field of paraffin embedding, and discloses a paraffin embedding device for pathological sections and a control method thereof. The device comprises a workbench, a wax cavity, a movable groove and a heat dissipation cavity, wherein the wax filling device comprises a first wax dripping machine and a second wax dripping machine, the refrigeration platform comprises a first refrigeration plate and a second refrigeration plate, the transportation part comprises a roller and a roadblock assembly, the temperature control device adopts a refrigeration system consisting of a compressor, a condensing coil, a throttling mechanism and an evaporating coil, wherein part of the condensing coil is arranged in the wax cavity to provide heat, the other part of the condensing coil is arranged in the heat dissipation cavity to dissipate heat, and the evaporating coil is used for refrigerating the refrigeration platform. The invention effectively improves the embedding quality and efficiency by combining staged embedding and freezing processes and automatic transportation, and simultaneously utilizes the waste heat of the refrigerating system to heat paraffin, thereby realizing the recycling of energy sources and having the advantages of energy conservation, environmental protection, simple and convenient operation, stable embedding quality and the like.

Inventors

YIN MEIFANG
LIU XIA
LI YONGTING
HUANG PING
LI HONGWU
CHEN XIAOLING
DONG LIRU
SHEN JIA

Assignees

深圳市第二人民医院(深圳市转化医学研究院)

Dates

Publication Date: 20260508
Application Date: 20251218

Claims (10)

1. The paraffin embedding device for pathological sections is characterized by comprising a workbench (1), a paraffin filling device (2), a conveying component (3), a freezing table (4), temperature control equipment (5) and an embedding mould (6); the workbench (1) is provided with a wax cavity (101), the refrigerating table (4) and the conveying component (3) are arranged on the workbench (1), and the wax cavity (101) is communicated with the wax filling device (2); the wax filling device (2) comprises a first wax dropping machine (201) and a second wax dropping machine (202), and the freezing table (4) comprises a first freezing plate (401) and a second freezing plate (402); The first freezing plate (401) is arranged below the first wax dropping machine (201) and is used for primarily embedding and freezing the tissue blocks of the embedding mould (6); The second wax dropping machine (202) is arranged above the conveying component (3) and is used for completely embedding the preliminarily embedded tissue blocks; The end part of the conveying component (3) is provided with a second freezing plate (402) for carrying out final freezing treatment on an embedding mould (6) for completely embedding the tissue block on the second freezing plate (402); the refrigerating part of the temperature control equipment (5) is connected with the refrigerating table (4), and the heating part of the temperature control equipment is connected with the wax cavity (101).
2. Paraffin embedding device for pathological sections according to claim 1, characterized in that the temperature control equipment (5) comprises a compressor (501), a condensing coil (502), a throttling mechanism (503) and an evaporating coil (504), wherein the outlet of the compressor (501) is connected with the condensing coil (502), the condensing coil (502) is connected with the evaporating coil (504) through the throttling mechanism (503), part of the condensing coil (502) is arranged in the wax cavity (101) to provide heat for the wax cavity (101), the evaporating coil (504) is arranged to fit with the freezing table (4), and the freezing table (4) is refrigerated to solidify wax in the embedding mould (6).
3. Paraffin embedding device for pathological sections according to claim 2, characterized in that the workbench (1) is further provided with a heat dissipation cavity (102), part of the condensing coil (502) is further arranged in the heat dissipation cavity (102), the heat dissipation cavity (102) is provided with a convection opening, and heat exchange is performed through a fan (7).
4. A paraffin embedding device for pathological sections according to claim 3, characterized in that the workbench (1) is further provided with a moving groove (103), the transporting component (3) comprises a plurality of rollers (301), the rollers (301) are arranged on the moving groove (103) in rows, and the rollers (301) are further connected with a motor in a transmission manner and are used for enabling the embedding mold (6) to be transported automatically along the rows of rollers (301).
5. Paraffin embedding device for pathological sections according to claim 4, characterized in that the bottom of the mobile trough (103) is also detachably provided with a wax receiving box (8), the wax receiving box (8) being located below the roller (301) for receiving wax drops scattered during the embedding process.
6. Paraffin embedding device for pathological sections according to claim 5, characterized in that the transport means (3) further comprise a barrier assembly (302), which barrier assembly (302) is arranged below the second wax-dripping machine (202) for blocking and positioning an embedding mould (6) moving on the roller (301) below the second wax-dripping machine (202) for wax-receiving.
7. Paraffin embedding device for pathological sections according to claim 1, characterized in that the first freezing plate (401) is further provided with a mobile mounting table (9) for placing an embedding mould (6), the mobile mounting table (9) comprising a receiving plate (901), an elastic rack (902) and a spring rack (903); The elastic frame (902) is arranged at the bottom of the bearing plate (901) and is used for providing a reset force when the bearing plate (901) moves downwards; the receiving plate (901) is arranged above the first freezing plate (401) through the spring frame (903).
8. Paraffin embedding device for pathological sections according to claim 1, characterized in that the embedding mould (6) comprises a metal bottom box (601) for embedding pathological sections and a grid cover (602), wherein the grid cover (602) can be sleeved above the metal bottom box (601).
9. A control method using the paraffin embedding device as set forth in claim 6, wherein the control method of the paraffin embedding device includes: S1, opening temperature control equipment (5), placing an embedding mould (6) carrying a tissue block on a first freezing plate (401) after the temperature reaches a proper temperature, starting a first wax dropping machine (201) to drop molten paraffin into the embedding mould (6) for preliminary embedding, and simultaneously refrigerating the first freezing plate (401) by the temperature control equipment (5) to solidify the paraffin preliminarily embedded; S2, transferring the embedding mould (6) subjected to preliminary embedding and freezing to the initial end of the conveying part (3), and moving the embedding mould (6) under the rotation of the roller (301); S3, when the embedding mould (6) moves to the lower part of the second wax dropping machine (202), the second wax dropping machine (202) drops molten paraffin into the embedding mould (6) to be completely embedded by being blocked and positioned by the roadblock assembly (302); S4, when the molten paraffin dripped into the embedding mould (6) is completely embedded, the barrier of the roadblock assembly (302) is released, the completely embedded embedding mould (6) continues to move to the end part of the conveying part (3) and reaches the second freezing plate (402) for final freezing treatment, and after the paraffin is completely solidified, the embedded embedding mould (6) is taken down.
10. Paraffin embedding device for pathological sections according to claim 9, characterized in that in step S2, the scattered wax drops are received by a wax receiving box (8) located below the roller (301).

Description

Paraffin embedding device for pathological section and control method thereof Technical Field The invention relates to the field of paraffin embedding, in particular to a paraffin embedding device for pathological sections and a control method thereof. Background In pathological diagnosis and research, paraffin pathological section technology is the core and foundation for preparing histological specimens. The technology prepares biological tissues into slices which can be observed under a microscope through a series of strict steps including sample material taking, fixing, dehydration, transparency, wax dipping, embedding, slicing, spreading, dyeing and the like. The paraffin embedding link is particularly critical, and aims to fully submerge and fixedly seal the tissue blocks subjected to the pretreatment in paraffin to form the paraffin blocks with certain hardness and toughness, so that necessary structural support is provided for subsequent high-quality ultrathin sections. The conventional paraffin embedding operation is highly dependent on manual work, and the basic flow is that an operator places a tissue block immersed with paraffin at the bottom of a specific metal mold, then manually injects molten paraffin into the mold, and finally covers the mold and transfers the mold to a freezing table for rapid cooling and solidification. However, the paraffin dosage is difficult to accurately control, the paraffin filling amount is completely judged by the personal experience of operators, excessive paraffin filling amount is easy to overflow, waste paraffin and pollute the working table surface and the environment, or insufficient paraffin filling amount leads to poor wax block forming and insufficient tissue support, thereby generating the problems of cracks, wrinkles and the like during slicing, and seriously affecting the quality of the final slice and the accuracy of diagnosis. To address the above problems, some automated or semi-automated paraffin embedding devices are on the market. However, existing automation devices still have significant shortcomings in the functionality of the key links. They generally lack modules capable of achieving ordered, localized transfer and uniform cooling, resulting in limited effectiveness in improving standardization levels and batch processing efficiencies. Therefore, developing a paraffin embedding device that can achieve orderly transfer and uniform cooling to meet the increasing high throughput, standardization and high quality requirements of pathology laboratories has become a technical problem to be solved in the art. Disclosure of Invention The present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide a paraffin embedding device for pathological sections; another object of the present invention is to provide a method for controlling a paraffin embedding device. The invention is realized by adopting the following technical scheme: a paraffin embedding device for pathological sections comprises a workbench, a paraffin filling device, a conveying part, a freezing table, temperature control equipment and an embedding mould; The workbench is provided with a wax cavity, the refrigerating table and the conveying part are arranged on the workbench, and the wax cavity is communicated with the wax filling device; the wax filling device comprises a first wax dropping machine and a second wax dropping machine, and the freezing table comprises a first freezing plate and a second freezing plate; the first freezing plate is arranged below the first wax dropping machine and is used for primarily embedding and freezing the tissue blocks of the embedding mould; the second wax dropping machine is arranged above the conveying component and is used for completely embedding the preliminarily embedded tissue blocks; the end part of the conveying component is provided with a second freezing plate, and the second freezing plate is used for conveying an embedding mould for completely embedding the tissue blocks to the second freezing plate for final freezing treatment; The refrigerating part of the temperature control equipment is connected with the refrigerating table, and the heating part of the temperature control equipment is connected with the wax cavity. The temperature control equipment comprises a compressor, a condensing coil, a throttling mechanism and an evaporating coil, wherein an outlet of the compressor is connected with the condensing coil, the condensing coil is connected with the evaporating coil through the throttling mechanism, a part of the condensing coil is arranged in the wax cavity to provide heat for the wax cavity, and the evaporating coil is attached to the refrigerating table to refrigerate the refrigerating table so as to solidify wax in the embedding mould. Further, a heat dissipation cavity is further formed in the workbench, part of the condensing coil is fu