CN-121994760-A - Tissue fluorescence die body with controllable depth structure and preparation method and application thereof

CN121994760ACN 121994760 ACN121994760 ACN 121994760ACN-121994760-A

Abstract

The invention discloses a tissue fluorescent die body with a controllable depth structure, and a preparation method and application thereof, and belongs to the technical field of medical imaging and optical detection. The tissue fluorescent die body is prepared from a multi-component optical simulation material and a die, wherein the material comprises, by weight, 0.01-0.05% of a melanin solution, 0.01-0.1% of polystyrene microspheres, 0.03-0.08% of a fluorescent agent, 1.0-1.5% of a coagulant and the balance of a liquid matrix. The tissue fluorescence model has the beneficial effects that the tissue fluorescence model can simulate tissue absorption, scattering and endogenous fluorescence characteristics at the same time, so that the optical behavior of the tissue fluorescence model is more similar to that of a real biological tissue. The die body design with the small holes with controllable structured depth ensures that the exogenous fluorescence can be distributed at different depths according to the requirement, thereby simulating the depth gradient fluorescence signal in real tissues. The preparation process is simple, high in repeatability, easy to standardize and suitable for mass production.

Inventors

NI JINGSHU
WANG YIKUN
DONG MEILI
He Tengchao
LIU YONG

Assignees

中国科学院合肥物质科学研究院
合肥易康达医学科技有限公司

Dates

Publication Date: 20260508
Application Date: 20251231

Claims (10)

1. A multi-component optical simulation material is characterized by comprising, by weight, 0.01-0.05% of a melanin solution, 0.01-0.1% of polystyrene microspheres, 0.03-0.08% of a fluorescent agent, 1.0-1.5% of a coagulant and the balance of a liquid matrix.
2. The multicomponent optical modeling material of claim 1, wherein the fluorescent agent is reduced coenzyme I or flavin adenine dinucleotide, the clotting agent is agar or gelatin, and the liquid matrix is purified water or PBS solution.
3. The method of preparing a tissue phantom from a multicomponent optical modeling material of claim 1, comprising the steps of: (1) Mixing melanin solution, polystyrene microsphere, coagulant and liquid matrix, heating, adding fluorescent agent, and mixing; (2) Pouring the mixed solution obtained in the step (1) into a die A, covering a die B, standing until the mixed solution is completely solidified, and demolding to obtain the finished product.
4. A method according to claim 3, wherein the heating is in particular heating to boiling.
5. A method according to claim 3, wherein the mould a is matched with a mould B in which a plurality of pillars of different heights are provided.
6. A tissue fluorescent motif produced by the method of any one of claims 3-5.
7. Use of the tissue fluorescent phantom of claim 6 in an evaluation of an imaging device.
8. The use of claim 7, wherein the items assessed by the imaging device include lateral resolution of the fluoroscopic imaging system, depth imaging performance and penetration capability, fluorescence quantification accuracy, multispectral imaging algorithm verification, imaging system signal-to-noise assessment.
9. The die assembly for preparing the tissue fluorescent die body is characterized by comprising a die A and a die B, wherein the die A is matched with the die B, a plurality of stand columns with different heights are arranged in the die B, and the stand columns are conical stand columns with a die drawing angle of 1-3 degrees.
10. The mold assembly according to claim 9, wherein the mold a is made of any one of heat-resistant glass and stainless steel, the mold a is in any one of a cylinder, a cuboid, a cube and a cone, the mold a is used for containing liquid mold body materials, and the mold B is used for being inserted into a mold body to form small holes with different depths.

Description

Tissue fluorescence die body with controllable depth structure and preparation method and application thereof Technical Field The invention belongs to the technical field of medical imaging and optical detection, relates to a tissue optical die body for performance evaluation, calibration and verification of a fluorescence imaging system, and in particular relates to a tissue fluorescence die body with a depth-controllable structure, and a preparation method and application thereof. Background The fluorescence imaging technology is widely applied to the fields of clinical infection detection, tumor imaging, photodynamic therapeutic agent distribution detection, biochemical metabolism research and the like. In order to accurately assess the depth penetration capability, fluorescence quantification accuracy, and image processing algorithm performance of a fluorescence imaging device, it is often necessary to calibrate and verify the device using tissue optical motifs. The prior art has the following defects that 1, most die bodies only have absorption and scattering characteristics and lack adjustable fluorescent characteristics. Traditional motifs such as agar motifs, PVA-C motifs, intralipid motifs, etc. are used only for optical parameter simulation and cannot reproduce the endogenous fluorescence of solid tissues (e.g.NADH, etc.). 2. Most of the fluorescent die bodies are of an integral uniform structure, and fluorescent signals with different tissue depths cannot be simulated. Due to the lack of depth structure, it is difficult to use for depth-resolved performance testing of devices. 3. There is a lack of a method for preparing a phantom with a precisely repeatable geometry. 4. The die body structure which can support the porous depth and bear a plurality of fluorescent reagents at the same time is not established, so that the functional limitation is caused, and the integral performance verification requirement of imaging equipment with different wavelengths cannot be met. Therefore, a tissue fluorescent motif capable of simultaneously modeling absorption, scattering, endogenous fluorescence and having a controlled depth pore structure is needed to address the lack of standardized testing of device depth imaging performance. The Chinese patent application document with publication number of CN116124752A discloses a tissue bionic die body based on multispectral regulation and control and a generation method thereof, wherein the die body lacks endogenous background fluorescent substances and necessary absorbent and scattering agent components, and cannot truly simulate the complex optical environment of biological tissues, meanwhile, the die body is of a uniform integral structure, lacks a channel system with controllable depth, cannot simulate fluorescent distribution with different depths on the same die body, does not support dynamic replacement of different fluorescent reagents in the test process, and is difficult to meet the actual requirements of depth resolution and comprehensive performance evaluation of fluorescent imaging equipment. Disclosure of Invention The invention aims to solve the technical problems of single simulation function and poor simulation result accuracy of the existing tissue fluorescent die body. The invention aims to solve the following technical problems in the existing tissue fluorescent die body: 1. It is difficult to simulate the absorption, scattering and endogenous fluorescence characteristics of real tissues at the same time, and the existing die body can only simulate one of the scattering or absorption characteristics of the tissues, or only add exogenous fluorescence but lack endogenous fluorescence components, so that the simulation result has obvious difference with the optical behaviors of the real tissues, and the evaluation accuracy of imaging equipment is affected. 2. The existing die body is generally of a uniform integral structure, and cannot form a small-scale space structure with controllable depth and repeatability in the die body for simulating fluorescent signals generated at different depths in tissues, so that the deep detection capability and the depth resolution performance of equipment cannot be systematically evaluated. 3. The preparation process of the die body is complex, and the standardization and the mass production are inconvenient, wherein the existing method needs manual layering, repeated pouring and other steps, has complicated operation and is greatly influenced by human factors, so that the consistency among the die bodies is poor, and the requirements of laboratory standardization, mass production of equipment enterprises and clinical verification are difficult to meet. 4. The die body has single function, cannot support the multi-index performance evaluation of imaging equipment, wherein the traditional die body is mainly used for simple luminescence simulation, can not synchronously realize absorption, scattering, endogenous fluo