CN-121994762-A - Three-dimensional fluorescence spectrometer and pollution tracing method thereof

CN121994762ACN 121994762 ACN121994762 ACN 121994762ACN-121994762-A

Abstract

The invention belongs to the technical field of analysis and detection, and particularly relates to a three-dimensional fluorescence spectrometer and a pollution tracing method thereof, wherein the three-dimensional fluorescence spectrometer comprises a light source module, a light source monochromator, a fluorescence monochromator, a sample cell and a PMT detector, the light source monochromator and the fluorescence monochromator are respectively provided with an incident slit and an emergent slit, and the incident slit and the emergent slit are respectively of a slit continuous adjustable structure; the pollution tracing method comprises the steps of constructing a three-dimensional fluorescence spectrum database of a pollution source, performing operations such as data acquisition, pretreatment and the like on an acquired water sample, training to obtain an optimal BiA-GRU-LSTM tracing model, inputting newly acquired sample data into the tracing model, and being applicable to multi-scene pollution tracing of surface water, industrial wastewater and the like, wherein the type and the characteristics of the pollution source can be rapidly identified.

Inventors

XU TAO
ZHOU LEI
GU JIASHENG
CHEN TING
TANG HUAIWU
ZHOU WENJIE
LIAO CHANGYI
ZHENG XIANLEI

Assignees

杭州泽天春来科技股份有限公司

Dates

Publication Date: 20260508
Application Date: 20260116

Claims (10)

1. The three-dimensional fluorescence spectrometer comprises a light source module, a light source monochromator, a fluorescence monochromator, a sample cell and a PMT detector, wherein the light source monochromator and the fluorescence monochromator are respectively provided with an incident slit and an emergent slit, and the three-dimensional fluorescence spectrometer is characterized in that the incident slit and the emergent slit are respectively in a slit continuously adjustable structure; The slit continuously adjustable structure comprises a base frame, a slit opening, a first slit adjusting block, a second slit adjusting block, a slit adjusting gear, a transmission gear and a stepping motor, wherein the slit opening, the first slit adjusting block, the second slit adjusting block, the slit adjusting gear, the transmission gear and the stepping motor are arranged on the base frame, the slit adjusting gear and the slit opening are coaxially distributed, the slit adjusting gear is meshed with the transmission gear, and the transmission gear is in driving connection with the stepping motor; The first slit adjusting block and the second slit adjusting block are both movably connected with two parallel guide posts, and the guide posts are fixedly arranged on the base frame; the first slit adjusting block and the second slit adjusting block are distributed on two sides of the slit opening, the two guide posts are respectively sleeved with a reset spring, two ends of one reset spring are respectively abutted between the first slit adjusting block and the base frame, and two ends of the other reset spring are respectively abutted between the second slit adjusting block and the base frame; The slit adjusting gear is provided with a first limit column and a second limit column along two ends of the middle axis, the first limit column is abutted to the second slit adjusting block, the second limit column is abutted to the first slit adjusting block, and the first slit adjusting block and the second slit adjusting block are adjusted to be close to or far away from each other relative to the slit opening through rotation of the slit adjusting gear so as to adjust the width of the slit.
2. The three-dimensional fluorescence spectrometer according to claim 1, wherein the first slit adjusting block comprises a first movable connection block and a first slit sheet provided on the first movable connection block, the first slit sheet being for shielding a slit opening; the second slit adjusting block comprises a second movable connecting block and a second slit sheet arranged on the second movable connecting block, and the second slit sheet is used for shielding a slit; The first slit sheet and the second slit sheet are close to or far away from each other so as to adjust the slit width.
3. The three-dimensional fluorescence spectrometer according to claim 2, wherein the first movable connection block is provided with a first extending abutting arm, the first abutting arm and the first movable connection block are respectively positioned at two sides of the slit, and the second limiting column abuts against one side of the first abutting arm away from the first movable connection block; The second movable connecting block is provided with a second abutting arm which extends, the second abutting arm and the second movable connecting block are respectively positioned at two sides of the slit, and the first limiting column abuts against one side, far away from the second movable connecting block, of the second abutting arm.
4. The three-dimensional fluorescence spectrometer of claim 3, wherein the first slit adjusting block and the second slit adjusting block are centered with respect to each other.
5. The three-dimensional fluorescence spectrometer of any of claims 1-4, wherein the first and second stop posts are bearing structures.
6. The three-dimensional fluorescence spectrometer of any of claims 1-4, wherein the base frame is provided with a positioning optocoupler, and the first slit adjusting block or the second slit adjusting block is provided with an optocoupler baffle, and the optocoupler baffle is matched with the positioning optocoupler for adjusting the slit width.
7. The three-dimensional fluorescence spectrometer of any of claims 1-4, wherein the base frame is further provided with a filter turntable and a plurality of bandpass filters circumferentially arranged on the filter turntable, the filter turntable is in driving connection with the driving motor, and the bandpass filters are switched to correspond to the slit openings by rotation of the filter turntable.
8. The three-dimensional fluorescence spectrometer of claim 7, wherein the pedestal is further provided with a positioning sensor for positioning the bandpass filter center in alignment with the slit aperture center.
9. The pollution tracing method of a three-dimensional fluorescence spectrometer of any one of claims 1-8, comprising the steps of: s1, acquiring a three-dimensional fluorescence spectrum data set through a three-dimensional fluorescence spectrometer and preprocessing data; S2, carrying out parallel factorization on the three-dimensional fluorescence spectrum data after the data pretreatment, and decomposing the three-dimensional fluorescence spectrum data into an excitation load matrix, an emission load matrix and a relative concentration load matrix to form a characteristic data set; S3, training a BiA-GRU-LSTM model by utilizing a characteristic data set to obtain a traceability model, wherein the BiA-GRU-LSTM model comprises Bi-GRU and Bi-LSTM which are arranged in parallel, the Bi-GRU and the Bi-LSTM are output and spliced by characteristic dimensions, multi-head attention is added, attention weight is weighted and aggregated, weighted attention characteristic representation is generated, layer normalization and batch standardization processing are sequentially carried out, a plurality of repeated processing layers are processed, and a model result is output by matching with a ReLU activation function, wherein the repeated processing layers comprise linear transformation, batch standardization and dropout layer processing in sequence; S4, acquiring three-dimensional fluorescence spectrum data of the water sample to be identified through a three-dimensional fluorescence spectrometer, preprocessing the data, decomposing parallel factors, inputting a traceability model for prediction, and outputting a traceability result.
10. The data processing method according to claim 9, wherein the tracing result includes an emission source name with highest matching degree, a matching degree value and a confidence interval; when the predicted matching degree is more than or equal to a preset threshold value, judging that the water sample to be identified is derived from a corresponding emission source; And when the matching degree is less than a preset threshold value, judging that the water sample is an unknown source water sample or a mixed pollution water sample.

Description

Three-dimensional fluorescence spectrometer and pollution tracing method thereof Technical Field The invention belongs to the technical field of analysis and detection, and particularly relates to a three-dimensional fluorescence spectrometer and a pollution tracing method thereof. Background The three-dimensional fluorescence spectrum technology is a spectrum analysis technology based on the fluorescence characteristic of a substance, and realizes qualitative and quantitative analysis and traceable identification of fluorescent components in a sample by synchronously collecting data of three dimensions of excitation wavelength, emission wavelength and corresponding fluorescence intensity of the fluorescent substance to construct an excitation-emission-intensity three-dimensional spectrum, also called a fluorescence fingerprint. The technology is widely applied to the field of environmental monitoring by virtue of the advantages of high sensitivity, non-destructiveness, multi-component simultaneous analysis and the like, and particularly becomes one of core technologies in the tracing of organic pollutants in water. In practical application, however, the slit width is fixed or is adjusted in a multi-stage discrete manner, continuous adjustment cannot be achieved, in addition, characteristic peaks with close peak positions cannot be distinguished through simple pretreatment in the prior art, the tracing deviation is large due to overlapping and shielding of fluorescent peaks, the accuracy is low, and the prior art relies on a large amount of marking data and is prone to failure in the face of different process waste water. Disclosure of Invention Based on the above-mentioned drawbacks and deficiencies of the prior art, it is an object of the present invention to at least solve one or more of the above-mentioned problems of the prior art, in other words, to provide a three-dimensional fluorescence spectrometer and a pollution tracing method thereof that meet one or more of the above-mentioned needs. In order to achieve the aim of the invention, the invention adopts the following technical scheme: the three-dimensional fluorescence spectrometer comprises a light source module, a light source monochromator, a fluorescence monochromator, a sample cell and a PMT detector, wherein the light source monochromator and the fluorescence monochromator are respectively provided with an incident slit and an emergent slit, and the incident slit and the emergent slit are respectively in a slit continuously adjustable structure; The slit continuously adjustable structure comprises a base frame, a slit opening, a first slit adjusting block, a second slit adjusting block, a slit adjusting gear, a transmission gear and a stepping motor, wherein the slit opening, the first slit adjusting block, the second slit adjusting block, the slit adjusting gear, the transmission gear and the stepping motor are arranged on the base frame, the slit adjusting gear and the slit opening are coaxially distributed, the slit adjusting gear is meshed with the transmission gear, and the transmission gear is in driving connection with the stepping motor; The first slit adjusting block and the second slit adjusting block are both movably connected with two parallel guide posts, and the guide posts are fixedly arranged on the base frame; the first slit adjusting block and the second slit adjusting block are distributed on two sides of the slit opening, the two guide posts are respectively sleeved with a reset spring, two ends of one reset spring are respectively abutted between the first slit adjusting block and the base frame, and two ends of the other reset spring are respectively abutted between the second slit adjusting block and the base frame; The slit adjusting gear is provided with a first limit column and a second limit column along two ends of the middle axis, the first limit column is abutted to the second slit adjusting block, the second limit column is abutted to the first slit adjusting block, and the first slit adjusting block and the second slit adjusting block are adjusted to be close to or far away from each other relative to the slit opening through rotation of the slit adjusting gear so as to adjust the width of the slit. As a preferable scheme, the first slit adjusting block comprises a first movable connecting block and a first slit sheet arranged on the first movable connecting block, wherein the first slit sheet is used for shielding a slit; the second slit adjusting block comprises a second movable connecting block and a second slit sheet arranged on the second movable connecting block, and the second slit sheet is used for shielding a slit; The first slit sheet and the second slit sheet are close to or far away from each other so as to adjust the slit width. As a preferable scheme, the first movable connecting block is provided with a first abutting arm which extends, the first abutting arm and the first movable connecting blo