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CN-121994779-A - Determination method for nutrient digestibility of mineral elements of pig feed

CN121994779ACN 121994779 ACN121994779 ACN 121994779ACN-121994779-A

Abstract

The invention discloses a method for measuring the nutrient digestibility of mineral elements in pig feed. The method comprises the steps of selecting a test pig with a T-shaped fistula at the tail end of the ileum and the tail end of the cecum, preparing feed containing an indicator, setting a test period comprising a pre-test period, a fecal collecting period, a cecum chyme collecting period and an ileum chyme collecting period, feeding the test pig with the prepared feed, respectively collecting a feed sample, a fecal sample, a cecum chyme sample and an ileum chyme sample in the collecting period, measuring the mineral element content and the indicator content in the feed sample, the fecal sample, the cecum chyme sample and the ileum chyme sample, and calculating the apparent digestibility of mineral elements at different parts of the alimentary canal of the pig according to a formula.

Inventors

  • WAN DAN
  • GAO XINYI
  • LI RUI

Assignees

  • 中国科学院亚热带农业生态研究所

Dates

Publication Date
20260508
Application Date
20260107

Claims (10)

  1. 1. The method for determining the nutrient digestibility of the mineral elements of the pig feed is characterized by comprising the following steps of: (1) Selecting a test pig with T-shaped fistulae at the ileum terminal and the cecum terminal, and preparing a feed containing an indicator; (2) Setting a test period comprising a pre-test period, a fecal collection period, a cecal chyme collection period and an ileal chyme collection period, and feeding the test pigs in the step (1) with the feed prepared in the step (1); (3) Respectively collecting a feed sample, a fecal sample, a cecum chyme sample and an ileum chyme sample in the collecting period in the step (2); (4) Determining the mineral element content and the indicator content in a feed sample, a fecal sample, a cecal chyme sample and an ileal chyme sample; (5) The apparent digestibility of mineral elements at different parts of the pig digestive tract is calculated according to the following formula:
  2. 2. The method of determining nutrient digestibility of mineral elements in a pig feed of claim 1, wherein the indicator comprises one of TiO 2 、Cr 2 O 3 、Fe 2 O 3 、Ti 2 O 3 or BaSO 4 .
  3. 3. The method for determining the nutrient digestibility of mineral elements in a pig feed according to claim 2, wherein the indicator is TiO 2 .
  4. 4. The method for determining the nutrient digestibility of mineral elements in pig feed according to claim 1, wherein the indicator is 0.2-0.5% by mass of the feed.
  5. 5. The method for determining the nutrient digestibility of mineral elements in pig feed according to claim 4, wherein the indicator is 0.3% by mass of the feed.
  6. 6. The method for determining the nutrient digestibility of mineral elements in a pig feed according to claim 1, wherein the pre-test period in the test period is not less than 5 days, the fecal collection period is not less than 3 days, the cecal chyme collection period is not less than 2 days, and the ileal chyme collection period is not less than 2 days.
  7. 7. The method of determining nutrient digestibility of a mineral element of a pig feed of claim 1, wherein the mineral element comprises one of calcium, phosphorus, iron, zinc, copper, and manganese.
  8. 8. The method for determining the nutrient digestibility of mineral elements in pig diets according to claim 7, wherein the AID, ACD and ATTD determined by the indicator method are used to select a high value as a criterion for the mineral element digestibility or nutrient requirement parameter.
  9. 9. The method for determining the nutrient digestibility of mineral elements in pig diets according to claim 7, wherein the calcium and phosphorus are used as reference standards for apparent digestibility of calcium and phosphorus in the feed by ACD or ATTD, the iron is used as reference standards for apparent digestibility of iron in the feed by ATTD, and the zinc, copper and manganese are used as reference standards for apparent digestibility of zinc, copper and manganese in the feed by ACD.
  10. 10. The method for determining the nutrient digestibility of the mineral elements of the pig feed according to claim 1, wherein ATTD values measured by the indicator method and the total manure collecting method are mutually checked, and the relative error of ATTD values obtained by the two methods is less than or equal to 10%.

Description

Determination method for nutrient digestibility of mineral elements of pig feed Technical Field The invention belongs to the technical field of animal nutrition evaluation, and particularly relates to a method for measuring the nutrient digestibility of mineral elements in pig feed. Background In the field of accurate nutrition and green cultivation of live pigs, the in-vivo digestion and metabolism rate of mineral elements (such as iron, zinc, copper and manganese) in the diet is accurately estimated, and the method is a core precondition for optimizing the feed formula, improving the element utilization efficiency, reducing the environmental pollution and guaranteeing the animal health. Traditionally, slope ratio methods are often used for evaluation of the validity of pig ration mineral elements to obtain the relative biological titers of the mineral elements. However, the difference in standard mineral element sources greatly limits the accuracy of such an evaluation system and does not truly reflect the pig's need for mineral elements. The state of intestinal nutrient digestion and absorption can be characterized by collecting feces to determine the apparent digestibility of the nutrient in the whole intestinal tract (ATTD), and the obtained result can reflect the end-point digestibility value of the whole digestive tract, but the method has great challenges for the digestive metabolism rate of mineral elements. Since the hindgut (cecum, colon) has complex microbial fermentation activities and unavoidable endogenous mineral secretion and excretion, ATTD values are severely disturbed, and the endogenous excretion results in a failure to truly reflect the main digestion and absorption sites, i.e. the real digestion and absorption efficiency of the small intestine on mineral elements, due to the low content of mineral elements. To overcome hindgut interference, the ileal chyma may be collected directly by installing a T-fistula at the terminal ileum, and the Apparent Ileal Digestibility (AID) may be determined to more accurately assess the digestion and absorption of mineral elements in the small intestine. However, this method only provides data on foregut digestibility and has the key disadvantage that, first, it ignores digestion and absorption of mineral elements by hindgut microorganisms and fails to answer the change of mineral elements in the hindgut. Secondly, when novel nutrition strategies such as low-protein daily ration, high-fiber daily ration and the like are explored, the daily ration composition has obvious influence on the composition of the hindgut microorganisms and the fermentation activity, and further the solubility, chemical morphology and excretion of mineral elements can be influenced. Thus, single ileal digestibility data fails to reveal a complete metabolic profile of this foregut-to-foregut interaction. The prior art has a methodological slice of either general overall digestibility (ATTD, greatly disturbed by the hindgut) or isolated foregut absorption (AID, lack of hindgut metabolic information). This results in a lack of in vivo research methods that allow simultaneous, continuous resolution of mineral flow patterns throughout critical parts of the digestive tract when mineral nutrition is accurately assessed and nutrient interactions are studied. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides a method for measuring the nutrient digestibility of mineral elements in pig feed. In order to achieve the above purpose, the technical scheme provided by the invention is as follows: the method for measuring the nutrient digestibility of the mineral elements of the pig feed comprises the following steps: (1) Selecting a test pig with T-shaped fistulae at the ileum end and the cecum end, and preparing a feed containing an indicator (test growing pig feed is prepared according to the standard of pig nutrient requirement (2012); (2) Setting a test period comprising a pre-test period, a fecal collection period, a cecal chyme collection period and an ileal chyme collection period, and feeding the test pigs in the step (1) with the feed prepared in the step (1); (3) Respectively collecting a feed sample, a fecal sample, a cecum chyme sample and an ileum chyme sample in the collecting period in the step (2); (4) Determining the mineral element content and the indicator content in a feed sample, a fecal sample, a cecal chyme sample and an ileal chyme sample; (5) The apparent digestibility of mineral elements at different parts of the pig digestive tract is calculated according to the following formula: Preferably, the indicator includes, but is not limited to, one of TiO 2、Cr2O3、Fe2O3、Ti2O3 or BaSO 4. More preferably, the indicator is TiO 2. Preferably, the indicator is 0.2-0.5% by mass of the feed. More preferably, the indicator is 0.3% by mass of the feed. Preferably, the pre-test period in the test period is not less than 5 days, the fecal