CN-121994791-A - Imaging method, imaging device, electronic equipment and storage medium

CN121994791ACN 121994791 ACN121994791 ACN 121994791ACN-121994791-A

Abstract

The application discloses an imaging method, an imaging device, electronic equipment and a storage medium, wherein the imaging method comprises the following steps that an aperture diaphragm performs preliminary image acquisition of a sample on at least one hole site in an orifice plate device by matching with an imaging module with a preset aperture value; and respectively and correspondingly adjusting the actual aperture value of the aperture diaphragm according to the brightness difference value so as to respectively acquire the actual image of the sample at each position in the hole site by matching with the imaging module. The brightness difference between the central position and other different positions is obtained by the aperture diaphragm through the preset aperture value, and the actual aperture value of the aperture diaphragm is adjusted according to the brightness difference when photographing next time, so that other different positions can have large-angle light rays and concave liquid surface shapes to form a counteracting effect, the problem of edge blackening caused by the concave liquid surface formed by the liquid surface tension is solved, and the cell loss of an edge region during imaging is reduced.

Inventors

LI YUJIAN
QIN YONGRONG
LIN YUHUAN
SHI ZHENZHI

Assignees

广州市艾贝泰生物科技有限公司

Dates

Publication Date: 20260508
Application Date: 20260302

Claims (10)

1. An imaging method, comprising the steps of: the aperture diaphragm is matched with an imaging module to perform primary image acquisition of a sample on at least one hole site in the pore plate device according to a preset aperture value; calculating the brightness difference between the center position in the hole site and other different positions according to the preliminary image acquired by the preliminary image; And respectively and correspondingly adjusting the actual aperture value of the aperture diaphragm according to the brightness difference value so as to match the imaging module to respectively acquire the actual image of the sample at each position in the hole site.
2. The method of imaging of claim 1, wherein said performing a preliminary image acquisition of the sample comprises: and selecting a target hole site to be imaged, shooting the target hole site to obtain a plurality of images, and fusing the shot images after the shooting area covers the cross-sectional area of the hole site to obtain the preliminary image.
3. The method of imaging of claim 2, wherein calculating the difference in brightness between the center position and the other different positions in the hole site based on the preliminary image acquired from the preliminary image comprises: And carrying out region division on the primary image obtained by fusion based on the image brightness value to obtain a central region and an edge region, and calculating the difference between the image brightness value of the central region and the image brightness value of the edge region to obtain the brightness difference.
4. The imaging method as set forth in claim 3, wherein the step of performing actual image acquisition of the sample for each position in the hole site in cooperation with the imaging module comprises: Respectively acquiring actual images according to the central area and the edge area; when the central area performs actual image acquisition, the aperture diaphragm performs image acquisition by matching the preset aperture value with the imaging module; when the edge area is used for acquiring an actual image, the aperture diaphragm is adjusted by taking the brightness value of the central area during imaging as a target brightness value, so that the image is acquired by matching with the imaging module after the actual brightness value of the edge area reaches the target brightness value.
5. The imaging method according to claim 1, wherein the adjusting the actual aperture values of the aperture stops according to the luminance difference values respectively includes: And presetting a mapping relation between a brightness difference value and a target aperture value, determining the target aperture value according to the brightness difference value based on the mapping relation, and adjusting the actual aperture value of the aperture diaphragm to reach the target aperture value.
6. The imaging method according to claim 5, wherein the map includes aperture calculation formulas corresponding to different liquid levels, the corresponding aperture calculation formulas are determined according to actual liquid levels, and the target aperture value is determined according to the corresponding aperture calculation formulas and the luminance difference value.
7. An imaging device is characterized by comprising an imaging module, an illumination module and a control module; the illumination module comprises an aperture diaphragm with an adjustable aperture; the imaging module is matched with the illumination module to acquire images; The control module is configured to issue control instructions, and the imaging module and the illumination module are configured to respond to the control instructions, respectively, to implement the imaging method as claimed in any one of claims 1 to 6.
8. The imaging device of claim 7, wherein the aperture diaphragm is adjustable in aperture by means of stepless adjustment or shift adjustment.
9. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor implements the imaging method as claimed in any one of claims 1 to 6 when the computer program is executed by the processor.
10. A storage medium having stored thereon a computer program which, when executed by a processor, implements the imaging method according to any of claims 1 to 6.

Description

Imaging method, imaging device, electronic equipment and storage medium Technical Field The present application relates to the field of optical imaging technologies, and in particular, to an imaging method, an imaging device, an electronic apparatus, and a storage medium. Background The pore plate is used as a sample carrying container and applied to a microscopic imaging scene, and a microscopic imaging system is used for observing and analyzing samples in the pore plate, so that relevant data of the samples are obtained. However, in the actual imaging process of the pore plate, due to the action of the surface tension of the liquid, the sample liquid in the pore plate can form a concave liquid surface, the brightness of the edge of the concave liquid surface can be lower than that of the central area under the influence of the light absorption characteristic of the liquid, and the cell loss of the edge area in the imaging image is easy to cause, so that the quality of full-pore imaging and the accuracy of subsequent analysis are affected. In order to solve the imaging defect caused by the concave liquid surface, some related technologies propose to design diaphragms with different shapes, shape the light to compensate the influence of the concave liquid surface, but the method only can compensate the concave liquid surface with a specific shape, and as the size of the concave liquid surface is directly related to the sample adding amount of the pore plate, the method has good effect only under specific conditions and has the problem of poor universality. There are also some related techniques to compensate for the problem of meniscus by making action on the consumable and by the shape of the consumable, but this method requires the development of new consumables, which are costly when using the main stream consumable, both commonly used and universal. There are some related arts that solve the problem of meniscus by adding an iris between the imaged objective lens and tube lens, but this method actually reduces the numerical aperture of the objective lens, thereby reducing the resolution of the imaging system. Disclosure of Invention The application provides an imaging method which is used for effectively solving the technical problems of imaging defects and the like caused by a concave liquid surface when an orifice plate is imaged in the related art. An embodiment of the first aspect of the present application provides an imaging method, including the steps of: the aperture diaphragm is matched with an imaging module to perform primary image acquisition of a sample on at least one hole site in the pore plate device according to a preset aperture value; calculating the brightness difference between the center position in the hole site and other different positions according to the preliminary image acquired by the preliminary image; And respectively and correspondingly adjusting the actual aperture value of the aperture diaphragm according to the brightness difference value so as to match the imaging module to respectively acquire the actual image of the sample at each position in the hole site. Further, the performing preliminary image acquisition of the sample includes: and selecting a target hole site to be imaged, shooting the target hole site to obtain a plurality of images, and fusing the shot images after the shooting area covers the cross-sectional area of the hole site to obtain the preliminary image. Further, the calculating the brightness difference between the center position in the hole site and other different positions according to the preliminary image acquired by the preliminary image includes: And carrying out region division on the primary image obtained by fusion based on the image brightness value to obtain a central region and an edge region, and calculating the difference between the image brightness value of the central region and the image brightness value of the edge region to obtain the brightness difference. Further, the matching with the imaging module to collect actual images of the samples at each position in the hole site respectively includes: Respectively acquiring actual images according to the central area and the edge area; when the central area performs actual image acquisition, the aperture diaphragm performs image acquisition by matching the preset aperture value with the imaging module; when the edge area is used for acquiring an actual image, the aperture diaphragm is adjusted by taking the brightness value of the central area during imaging as a target brightness value, so that the image is acquired by matching with the imaging module after the actual brightness value of the edge area reaches the target brightness value. Further, the adjusting the actual aperture values of the aperture stop according to the brightness difference values includes: And presetting a mapping relation between a brightness difference value and a target aperture value, determining the t