CN-121994892-A - Detection test paper capable of detecting two biological indexes and detection method and system thereof

CN121994892ACN 121994892 ACN121994892 ACN 121994892ACN-121994892-A

Abstract

The application provides a detection test paper capable of detecting two biological indexes, and a detection method and a detection system thereof. The test paper at least comprises a basal layer, a working electrode, a recognition electrode and a coding electrode. The working electrode is arranged on the first side surface and is at least used for detecting a first biological index and a second biological index. The identification electrodes are arranged on the first side face, are arranged to be at least 3, and at least two of the identification electrodes have a conducting relationship to form an identification circuit with unique corresponding identification codes, and each identification code corresponds to one test paper type. The coding electrodes are arranged on the second side face, each coding electrode is provided with a unique identification code, any at least two coding electrodes are conducted to form a preset on-off relation, a unique corresponding correction code is formed based on the identification codes and the on-off relation, and the correction code is used for determining first correction information of the first working electrode and second correction information of the second working electrode.

Inventors

FAN LIANGWEI
WANG XUEFENG
SU CHEN
Gong Lingbo
LI CHENGTAO

Assignees

江苏跃凯生物技术有限公司
江苏鱼跃凯立特生物科技有限公司
浙江凯立特医疗器械有限公司

Dates

Publication Date: 20260508
Application Date: 20260409

Claims (13)

1. A test strip capable of detecting two biological indicators, the test strip comprising: a substrate layer having oppositely disposed first and second sides; the working electrode is arranged on the first side surface and at least comprises a first working electrode for detecting a first biological index and a second working electrode for detecting a second biological index different from the first biological index; the identification electrodes are arranged on the first side face and are spaced from the working electrode in the flowing direction of the sample, at least 3 identification electrodes are arranged, at least two of the identification electrodes have a conducting relationship to form an identification circuit with unique corresponding identification codes, each identification code corresponds to one test paper type, and The coding electrodes are arranged on the second side face, the number of the coding electrodes is at least 6, each coding electrode is provided with a unique identification code, any at least two coding electrodes are conducted to form a preset on-off relation, a unique corresponding correction code is formed based on the identification codes and the on-off relation, and the correction code is used for determining first correction information of the first working electrode and second correction information of the second working electrode.
2. The test strip of claim 1, wherein the first biological indicator corresponds to blood glucose and the second biological indicator corresponds to uric acid.
3. The test strip of claim 1, further comprising a plurality of test electrodes disposed on the first side, wherein any two of the test electrodes, the identification electrodes, or the encoding electrodes are turned on to form a power-on signal, and the test instrument switches from a power-off state to an operating state when receiving the power-on signal.
4. A test strip according to claim 3, wherein the sum of the number of the detection electrodes and the number of the identification electrodes is equal to the number of the code electrodes, and the positions of the electrodes disposed on the first side face and the second side face are disposed in one-to-one correspondence, and projections of the electrodes disposed in each pair of the corresponding arrangements at least partially overlap in a direction perpendicular to the first side face.
5. The test strip of claim 1, wherein the code electrodes comprise a first code electrode and a second code electrode, the identification code and the on-off relationship of the first code electrode are used to determine the first correction information, the identification code and the on-off relationship of the second code electrode are used to determine the second correction information, and the number of the first code electrode and the second code electrode is greater than 2.
6. The test strip of any one of claims 1-5, wherein the identification code is formed based on a preset position of the code electrode, each of the calibration codes corresponding to a set of conversion functions pre-stored in a test instrument, the conversion functions including a first conversion function for converting a first test parameter into the first biological indicator and a second conversion function for converting a second test parameter into the second biological indicator, the first test parameter and the second test parameter being initial parameters extracted by the first working electrode and the second working electrode, respectively.
7. The test strip of any one of claims 1-5, wherein each of the identification codes corresponds to a set of initial conversion functions pre-stored in the test instrument, each of the calibration codes corresponds to a set of calibration parameters pre-stored in the test instrument, and the first biological indicator and the second biological indicator are obtained based on the initial conversion functions, the calibration parameters, first detection parameters, and second detection parameters, the first detection parameters and the second detection parameters being initial parameters extracted by the first working electrode and the second working electrode, respectively.
8. A test strip according to claim 3, wherein any at least two of the coding electrodes constitute a first judgment electrode, any at least two of the detection electrodes or the identification electrodes constitute a second judgment electrode, the first judgment electrodes and the second judgment electrodes are equal in number and are arranged in a one-to-one correspondence, projections of the first judgment electrodes and the second judgment electrodes at least partially overlap in a direction perpendicular to the first side, one of the first judgment electrodes and the second judgment electrodes is in a connected state, and the other of the first judgment electrodes and the second judgment electrodes is in a disconnected state, and an insertion direction of the test strip is determined based on an electrical connection relationship between the first judgment electrodes and the second judgment electrodes and a detection instrument.
9. The test strip of claim 1, wherein a first reagent is immobilized on the surface of the first working electrode, a second reagent is immobilized on the surface of the second working electrode, and the first reagent is one of glucose oxidase and glucose dehydrogenase, and the second reagent is uricase.
10. The test strip of any one of claims 1 to 9, comprising the steps of: The method comprises the steps of starting up, namely electrically connecting any two conducting electrodes in the detection test paper with a conducting terminal of a detection instrument to form a starting-up signal, wherein the starting-up signal enables the detection instrument to be switched from a shutdown state to a working state; The detection test paper further comprises a first judgment electrode and a second judgment electrode, the first judgment electrode and the second judgment electrode are electrically connected with a conductive terminal of the detection instrument to form a plugging direction identification signal, and the detection instrument outputs the information of the insertion direction of the detection test paper based on the plugging direction identification signal; The identification electrode contacts with a conductive terminal of the detection instrument to form an identification code, and the detection instrument confirms the type of the test paper through the identification code; And determining correction information, namely electrically connecting the coding electrode with a conductive terminal of the detection instrument to form a correction code, and displaying the correction code by the detection instrument and determining first correction information of the first working electrode and second correction information of the second working electrode through the correction code.
11. The method of detecting according to claim 10, wherein the determining correction information further comprises: Each correction code corresponds to a set of conversion functions, the detection instrument converts a first detection parameter into the first biological index through a first conversion function in the conversion functions, converts a second detection parameter into a second biological index through a second conversion function in the conversion functions, and the first detection parameter and the second detection parameter are initial parameters extracted by the first working electrode and the second working electrode respectively.
12. The method of detecting according to claim 10, wherein the determining correction information further comprises, after: Each identification code corresponds to a set of initial conversion function, the detection instrument determines the initial conversion function through the identification code, converts a first detection parameter into the first biological index through a first initial conversion function and first correction information in the initial conversion function, converts a second detection parameter into a second biological index through a second conversion function and second correction information in the initial conversion function, and the first detection parameter and the second detection parameter are initial parameters extracted by the first working electrode and the second working electrode respectively.
13. A detection system capable of detecting two biological indicators, the detection system comprising: the test strip according to any one of claims 1 to 9; A detection instrument, the detection instrument comprising: A slot for receiving the test paper; The conductive terminal is arranged in the slot and is used for being electrically connected with the electrode when the detection test paper is inserted; a processor electrically connected with the conductive terminal; A memory and a computer program stored on the memory and capable of running on the processor; and the display screen is used for displaying the correction code, the first biological index and the second biological index.

Description

Detection test paper capable of detecting two biological indexes and detection method and system thereof Technical Field The application relates to the technical field of biosensors, in particular to a detection test paper capable of detecting two biological indexes, and a detection method and a detection system thereof. Background The electrochemical biosensor test paper is widely applied to the instant detection of biological indexes such as blood sugar, uric acid, ketone body and the like. Along with the improvement of the requirements of chronic patients such as diabetes, hypertension and the like on self health management, the multifunctional test paper capable of simultaneously detecting multiple indexes becomes an important development direction. The test paper can obtain at least two detection results simultaneously through one-time blood sampling and one-time sample adding, so that the detection efficiency is greatly improved, and the pain of a patient is relieved. However, the existing biosensor test paper with multi-index detection function may generate different detection signals on test papers of different batches due to raw material differences and production process fluctuations between test papers of different batches. For multi-index test paper, the problem is more complex, reagent components, reaction principles and sensitivities of a plurality of detection indexes are different, and the influence degree of batch fluctuation is inconsistent, so that independent correction information needs to be configured for each index. At present, the correction information or the confirmation and input mode has complicated and error-prone operation, or the read coding design logic of the correction information is complex and the quantity of the loadable correction information is limited, so that the correction requirement of multi-index detection is difficult to meet. More critically, the problem of mutual interference of electrode signals may exist when dense electrode layout and complex coding design are performed on the test paper with limited surface space, and for the multi-index test paper with more electrodes, the problem is more prominent, which not only affects the accuracy of the detection result, but also may cause failure in reading correction information. Based on this, how to provide a multi-index test paper with reasonable layout, which can accurately and efficiently read multi-correction information and bear more correction information, is a technical problem to be solved in the art. Disclosure of Invention In order to solve the problems, the embodiment of the application provides a detection test paper capable of detecting two biological indexes, a detection method and a detection system thereof, which are used for reasonably arranging electrodes of the multi-index detection test paper, realizing accurate and efficient multi-correction information reading and bearing more correction information, and effectively meeting the requirements of users on correction and detection of the multi-biological indexes. In a first aspect, an embodiment of the present application provides a test strip capable of detecting two biological indicators, the test strip comprising: a substrate layer having oppositely disposed first and second sides; the working electrode is arranged on the first side surface and at least comprises a first working electrode for detecting a first biological index and a second working electrode for detecting a second biological index different from the first biological index; the identification electrodes are arranged on the first side face and are spaced from the working electrode in the flowing direction of the sample, at least 3 identification electrodes are arranged, at least two of the identification electrodes have a conducting relationship to form an identification circuit with unique corresponding identification codes, each identification code corresponds to one test paper type, and The coding electrodes are arranged on the second side face, the number of the coding electrodes is at least 6, each coding electrode is provided with a unique identification code, any at least two coding electrodes are conducted to form a preset on-off relation, a unique corresponding correction code is formed based on the identification codes and the on-off relation, and the correction code is used for determining first correction information of the first working electrode and second correction information of the second working electrode. In one implementation of the application, the first biological indicator corresponds to blood glucose and the second biological indicator corresponds to uric acid. In one implementation manner of the application, the detection test paper further comprises a plurality of detection electrodes arranged on the first side face, any two electrodes of the detection electrodes, the identification electrodes or the coding electrodes are conducted to form a starting