CN-121994896-A - Stable multi-parameter determination kit for electrolyte analyzer

Abstract

The invention relates to a stable multi-parameter electrolyte analyzer assay kit, which comprises a solution A and a solution B, wherein the solution A comprises 80-160 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-100 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant. The solution B comprises 10-100 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-150 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant. The invention can simultaneously test the K + 、Na + 、Cl ‑ 、Ca 2+ 、Li + 、Mg 2+ and the pH content in the sample, the accuracy, the repeatability and the recovery rate are not affected when the sample is stored for 24 months at the temperature of 2-37 ℃, and the raw materials are easy to obtain.

Inventors

• CHEN ZIQI
• LI WEILI
• HUANG YANNI

Assignees

• 桂林优利特医疗电子有限公司

Dates

Publication Date

20260508

Application Date

20260227

Claims (5)

1. 1. A stable multi-parameter electrolyte analyzer is with survey kit, characterized by, including A liquid and B liquid; the solution A comprises the following components: 80-160 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-100 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant; The liquid B comprises the following components: 10-100 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-150 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant.
2. 2. The stable multiparameter electrolyte analyzer assay kit of claim 1, wherein the buffer is one or more of HEFES buffer, MES buffer, PBS buffer, tris-HCl buffer.
3. 3. The stable multiparameter electrolyte analyzer assay kit of claim 1, wherein the buffer has a pH of 6.80-7.60 at 25 ℃.
4. 4. The stable multiparameter electrolyte analyzer assay kit of claim 1, wherein the stabilizing agent is one or more of sorbitol, lactitol, sodium citrate, EDTA-Na2, EGTA.
5. 5. The stable multiparameter electrolyte analyzer assay kit according to any one of claims 1-4, wherein the surfactant is one or more of SB 3-10, tween-20, pluronic F-68, dodecyl polyethylene glycol ether.

Description

Stable multi-parameter determination kit for electrolyte analyzer Technical Field The invention relates to reagents in the field of in-vitro diagnosis, in particular to a stable measuring kit for a multiparameter electrolyte analyzer. Background The electrolyte is a main component of body fluid of a human body and participates in a plurality of important physiological and biochemical processes of the body. The detection of ion concentration plays an important role in the diagnosis of water, electrolyte metabolic disorders and acid-base imbalance, and is also indispensable for the monitoring of the body state in certain critical cases. The hypernatremia can be caused by absentmindedness, dysphoria, convulsion, epileptiform seizure, coma and death, and the hypokalemia can cause symptoms of digestive system, central nervous system, circulatory system and urinary system, and the hyperkalemia is mainly manifested by heart rate slowing, premature ventricular contraction, atrioventricular conduction block, ventricular fibrillation, cardiac arrest and the like. The electrolyte analyzer and the matched reagent thereof used in clinic at present contain less test items, the electrolyte analyzer containing lithium and magnesium channels is less, and the lack of the channels may lead to incomplete diagnosis, such as magnesium ion concentration affecting potassium and calcium metabolism, clinical hyponatremia is a common cause of lithium ion poisoning, hypokalemia can increase the risk of lithium ion poisoning, and for example, only test pH is not performed to test Cl -, although the problem of acid-base balance can be reflected, unbalanced cause cannot be assisted in positioning, such as respiratory acidosis caused by pneumonia, metabolic acidosis caused by diabetic ketosis and the like. Furthermore, these electrolyte analyzers have certain limitations on the requirements of the sample, and very few electrolyte analyzers can measure urine, plasma, whole blood and cerebrospinal fluid. For the matched reagent of the electrolyte analyzer, the pH change of the buffer solution can be caused by the temperature change, and the main components in the electrolyte reagent are acid-base buffer solution, inert inorganic salt and the like, so that the reagent is easy to breed microorganisms, and the problems of increased faults, reduced service life of an electrode and the like of the electrolyte analyzer can be caused, so that formaldehyde can be added to inhibit microorganisms conventionally. However, formaldehyde is harmful, so that research is now being conducted on developing reagents which do not contain formaldehyde or other volatile harmful substances, and the stability and the validity period of the reagents are improved by screening proper acid-base buffer solution, adding proper preservative, surfactant and the like, and meanwhile, the electrode film is friendly, so that the stability of the measurement result is ensured. Therefore, aiming at clinical demands, the development of a stable multi-parameter electrolyte analyzer assay kit with comprehensive test projects and sample types has important practical significance. Disclosure of Invention In summary, in order to overcome the defects of the prior art, the invention aims to provide a stable multi-parameter determination kit for an electrolyte analyzer, which is characterized in that under the condition of ensuring no influence on an electrode film, the stable multi-parameter determination kit for the electrolyte analyzer, which can simultaneously test various items (K +、Na+、Cl-、Ca2+、Li+、Mg2+, pH) and can test the sample types (serum, plasma, whole blood, urine, cerebrospinal fluid) comprehensively, ensures that all ions are not interfered with each other and improves the stability thereof by reasonably screening a buffer system, a preservative, a stabilizer, all kinds of ions and other components. The technical scheme for solving the technical problems is that the stable multi-parameter determination kit for the electrolyte analyzer comprises a solution A and a solution B; the solution A comprises the following components: 80-160 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-100 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant. Adding the components into purified water, and mixing to obtain solution A. The liquid B comprises the following components: 10-100 mmol/L of sodium chloride, 2-10 mmol/L of potassium chloride, 1-10 mmol/L of lithium acetate, 0.1-5 mmol/L of magnesium acetate, 0.1-4 mmol/L of anhydrous calcium chloride, 10-100 mmol/L of anhydrous sodium acetate, 50-150 mmol/L of buffer solution, 0.1-10 g/L of preservative, 0.1-10 g/L of stabilizer and 0.1-10 g/L of surfactant. Adding the components into purified water, and mixing to