CN-121994906-A - Ofloxacin concentration detection method, ofloxacin concentration detection device, electronic equipment and storage medium

CN121994906ACN 121994906 ACN121994906 ACN 121994906ACN-121994906-A

Abstract

The present application relates to the field of molecular imaging technologies, and in particular, to a method and an apparatus for detecting ofloxacin concentration, an electronic device, and a storage medium. The method comprises the steps of dropwise adding a preset matrix solution to the surface of a sample to be tested containing ofloxacin, drying in a preset drying mode to obtain a processed sample to be tested, carrying out ionization mass spectrometry imaging analysis on the processed sample to be tested in a preset ionization mode by using a preset imaging mass spectrometry microscope to obtain a current signal value, and determining the ofloxacin concentration in the sample to be tested based on a preset ofloxacin concentration-imaging signal value working curve and the current signal value. Therefore, the concentration detection of ofloxacin on the surface of goethite can be realized through the good linear relation between the ofloxacin concentration and the signal value of the imaging heat map, the problems of complex and time-consuming detection technology, more consumption of chemical reagents and the like in the prior art are solved, and the method has the characteristics of high sensitivity, small sample consumption, short analysis response time and the like.

Inventors

XU JING
TAO SHIYONG
LIN LIFANG
JIN LUYAO
CHEN YILIN
JI BINGBING

Assignees

武汉大学

Dates

Publication Date: 20260508
Application Date: 20241104

Claims (10)

1. The ofloxacin concentration detection method is characterized by comprising the following steps of: Obtaining a sample to be tested containing ofloxacin; Dropwise adding a preset matrix solution to the surface of the sample to be tested, and drying the sample by using a preset drying mode to obtain a treated sample to be tested; Carrying out ionization mass spectrometry imaging analysis on the processed sample to be tested in a preset ionization mode by using a preset imaging mass spectrometry microscope to obtain a current signal value; And determining the concentration of ofloxacin in the sample to be detected based on a preset ofloxacin concentration-imaging signal value working curve and the current signal value.
2. The method of claim 1, further comprising, prior to determining the ofloxacin concentration in the sample under test based on the preset ofloxacin concentration-imaging signal value operating curve and the current signal value: dripping ofloxacin solution with preset concentration on the surface of goethite of an initial conductive glass slide sample, and drying by utilizing the preset drying mode to obtain a first conductive glass slide sample; dropwise adding a preset matrix solution to the surface of the goethite of the first conductive slide glass sample, and drying by utilizing the preset drying mode to obtain a second conductive slide glass sample; Performing ionization mass spectrometry imaging analysis on the second conductive glass slide sample in the preset ionization mode by using the preset imaging mass spectrometry microscope to obtain a signal value at a mass-to-charge ratio corresponding to the ofloxacin solution with the preset concentration; And determining the preset working curve of the ofloxacin concentration-imaging signal value according to signal values of the ofloxacin solutions under different concentration conditions at corresponding mass-to-charge ratios.
3. The method of claim 2, further comprising, prior to dropping the predetermined concentration of ofloxacin solution on the goethite surface of the initial conductive slide sample: acquiring a target glass slide with conductivity; Attaching a conductive double-sided adhesive tape meeting a preset size to the surface of the target slide glass; and spreading a preset dose of goethite powder on the conductive double-sided adhesive tape on the surface of the target slide, and pressing and fixing to obtain an initial conductive slide sample with goethite adhered on the surface.
4. A method according to claim 3, wherein the ratio of the amount of ofloxacin solution added dropwise to the preset dose of goethite powder in each sample is 1mL/g.
5. The method of claim 3, wherein the target slide is an indium tin oxide slide.
6. A device for detecting the concentration of ofloxacin, characterized by comprising the following steps: the acquisition module is used for acquiring a sample to be detected containing ofloxacin; The processing module is used for dripping a preset matrix solution on the surface of the sample to be detected, and drying the sample to be detected by using a preset drying mode to obtain a processed sample to be detected; The analysis module is used for carrying out ionization mass spectrometry imaging analysis on the processed sample to be tested in a preset ionization mode by using a preset imaging mass spectrometry microscope to obtain a current signal value; And the determining module is used for determining the concentration of ofloxacin in the sample to be detected based on a preset ofloxacin concentration-imaging signal value working curve and the current signal value.
7. The apparatus of claim 6, wherein prior to determining the ofloxacin concentration in the sample under test based on the preset ofloxacin concentration-imaging signal value operating curve and the current signal value, the determining module further comprises: The first processing unit is used for dripping ofloxacin solution with preset concentration on the surface of goethite of the initial conductive slide sample, and drying the initial conductive slide sample by utilizing the preset drying mode to obtain a first conductive slide sample; the second processing unit is used for dropwise adding a preset matrix solution to the surface of the goethite of the first conductive slide glass sample, and drying the goethite by utilizing the preset drying mode to obtain a second conductive slide glass sample; The analysis unit is used for carrying out ionization mass spectrometry imaging analysis on the second conductive glass slide sample in the preset ionization mode by utilizing the preset imaging mass spectrometry microscope to obtain a signal value at a mass-to-charge ratio corresponding to the ofloxacin solution with the preset concentration; And the determining unit is used for determining the preset ofloxacin concentration-imaging signal value working curve according to signal values at the mass-to-charge ratios corresponding to the ofloxacin solutions under different concentration conditions.
8. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, the processor executing the program to implement the ofloxacin concentration detection method as claimed in any one of claims 1 to 5.
9. A computer-readable storage medium having stored thereon a computer program, characterized in that the program is executed by a processor for realizing the ofloxacin concentration detection method according to any one of claims 1 to 5.
10. A computer program product comprising a computer program for implementing the ofloxacin concentration detection method according to any one of claims 1 to 5 when executed by a processor.

Description

Ofloxacin concentration detection method, ofloxacin concentration detection device, electronic equipment and storage medium Technical Field The present application relates to the field of molecular imaging technologies, and in particular, to a method and an apparatus for detecting ofloxacin concentration, an electronic device, and a storage medium. Background Quinolone antibiotics are synthetic antibacterial agents for human and livestock containing 4-quinolone structures, and are also called as pyridonic acid or pyridonic acid antibiotics. The quinolone antibiotics are mostly discharged outside the body in a matrix form or a metabolic waste form after being used by human beings or animals to cause harm to the water body environment, can be discharged together with sewage, can also be discharged along with surface runoff or sewage treatment plant effluent and other modes, and finally enter the water environment. The organic ofloxacin (Ofloxacin, OFX for short) belongs to a typical fluoroquinolone antibiotic, and the substances are often used in industries such as medical treatment, cultivation and the like due to strong broad-spectrum antibacterial activity. Although ofloxacin is used for preventing and treating diseases, the residual ofloxacin in water environment has potential threat to aquatic plants, animals and even human beings, such as toxicity risk, generation of resistance genes, allergic reaction and the like. In the related art, chromatography is the most commonly used detection method for detecting ofloxacin concentration in environmental samples. The method is characterized in that the compound is separated into different components by utilizing the characteristics of different molecular weights, polarities, fluidity and the like of the compound, and by utilizing the instruments such as a high performance liquid chromatograph (High Performance Liquid Chromatography, for short, HPLC), an ultra-high performance liquid chromatograph (Ultra High Performance Liquid Chromatography, for short, UHPLC), a liquid chromatography-mass spectrometer (Liquid Chromatograph Mass Spectrometer, for short, LCMS) and the like, and the characteristic analysis is carried out by using a physical or chemical method, so that the identification and the quantification of chemical substances are realized. However, the method cannot directly measure the solid sample, only can convert the sample to be measured into completely dissolved liquid, and then the target substance to be measured is extracted through the modes of desorption, dissolution and the like, so that the treatment method is complex, time-consuming, more chemical reagents are consumed, and the method is needed to be solved. Disclosure of Invention The application provides a method, a device, electronic equipment and a storage medium for detecting ofloxacin concentration, which are used for solving the problems of complex and time-consuming detection technology, more consumption of chemical reagents and the like in the prior art, and have the characteristics of high sensitivity, small sample consumption, short analysis response time and the like. To achieve the above object, an embodiment of a first aspect of the present application provides a method for detecting ofloxacin concentration, comprising the steps of: Obtaining a sample to be tested containing ofloxacin; Dropwise adding a preset matrix solution to the surface of the sample to be tested, and drying the sample by using a preset drying mode to obtain a treated sample to be tested; Carrying out ionization mass spectrometry imaging analysis on the processed sample to be tested in a preset ionization mode by using a preset imaging mass spectrometry microscope to obtain a current signal value; And determining the concentration of ofloxacin in the sample to be detected based on a preset ofloxacin concentration-imaging signal value working curve and the current signal value. According to one embodiment of the present application, before determining the ofloxacin concentration in the sample to be measured based on the preset ofloxacin concentration-imaging signal value working curve and the current signal value, the method further comprises: dripping ofloxacin solution with preset concentration on the surface of goethite of an initial conductive glass slide sample, and drying by utilizing the preset drying mode to obtain a first conductive glass slide sample; dropwise adding a preset matrix solution to the surface of the goethite of the first conductive slide glass sample, and drying by utilizing the preset drying mode to obtain a second conductive slide glass sample; Performing ionization mass spectrometry imaging analysis on the second conductive glass slide sample in the preset ionization mode by using the preset imaging mass spectrometry microscope to obtain a signal value at a mass-to-charge ratio corresponding to the ofloxacin solution with the preset concentration; And determining the preset working cu