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CN-121994938-A - Construction method of five-finger wild peach decoction piece standard decoction fingerprint

CN121994938ACN 121994938 ACN121994938 ACN 121994938ACN-121994938-A

Abstract

The invention discloses a method for constructing a standard decoction fingerprint of a five-finger wild peach decoction piece, and relates to the technical field of traditional Chinese medicines. The construction method comprises the steps of adding psoralen and bergapten into a methanol aqueous solution with the volume fraction of 50% to prepare a reference substance solution, respectively carrying out water extraction treatment on batches of Ficus hirta to obtain different batches of standard decoction, respectively adding methanol into the different batches of standard decoction until the volume fraction of methanol is 50%, centrifuging to obtain supernatants to obtain different batches of sample solutions, respectively carrying out high performance liquid chromatography detection on the different batches of sample solutions to obtain fingerprint patterns, and calibrating characteristic peaks by using the reference substance solution. The method can accurately construct the standard decoction finger print of the Ficus simplicissima lour decoction pieces, thereby providing reference and basis for the overall quality control and evaluation of the standard decoction of the Ficus simplicissima lour decoction pieces and the formula particles.

Inventors

  • CHEN QING
  • LIN QINGHUA
  • JIANG LIN
  • LIN NING
  • LI FANGCHAN

Assignees

  • 广西中医药大学

Dates

Publication Date
20260508
Application Date
20231124

Claims (8)

  1. 1. The method for constructing the standard decoction fingerprint of the five-finger wild peach decoction pieces is characterized by comprising the following steps of: Adding psoralen and bergapten into 50% methanol water solution to obtain reference solution; Respectively extracting the five-finger nectarines in batches by water to obtain standard decoction in different batches, respectively adding methanol into the standard decoction in different batches until the volume fraction of the methanol is 50%, and centrifuging to obtain supernatant to obtain sample solutions in different batches; And respectively carrying out high performance liquid chromatography detection on the sample solutions in different batches to obtain fingerprint patterns, and calibrating characteristic peaks by using the reference solution.
  2. 2. The method for preparing the standard decoction according to claim 1, wherein the method for preparing the standard decoction comprises the steps of taking the Ficus simplicissima lour decoction pieces, soaking the decoction pieces in water with the weight being 8 times that of the decoction pieces for 30min, boiling and decocting the decoction pieces for 30min, filtering to obtain a first filtrate and filter residues, adding water with the weight being 6 times that of the filter residues, boiling and decocting the filter residues for 20min, filtering to obtain a second filtrate, merging the first filtrate and the second filtrate, and concentrating the first filtrate and the second filtrate under reduced pressure to obtain the standard decoction.
  3. 3. The method according to claim 1, wherein the high performance liquid chromatography is performed by XB-C18 chromatographic column.
  4. 4. The method according to claim 1, wherein the mobile phase detected by high performance liquid chromatography is methanol-0.1% phosphoric acid aqueous solution, wherein methanol is phase a and 0.1% phosphoric acid aqueous solution is phase B.
  5. 5. The method according to claim 4, wherein the gradient elution procedure for high performance liquid chromatography detection is as follows: 0-10 min,30% of phase A; 10-30 min, 30-50% of phase A; 30-55 min, 50-95% of phase A; 55-60 min,95% phase A.
  6. 6. The method according to claim 1, wherein the flow rate detected by high performance liquid chromatography is 1mL/min.
  7. 7. The method according to claim 1, wherein the detection wavelength of the high performance liquid chromatography is 245nm.
  8. 8. The method according to claim 1, wherein the column temperature detected by the high performance liquid chromatography is 30 ℃.

Description

Construction method of five-finger wild peach decoction piece standard decoction fingerprint Technical Field The invention relates to the technical field of traditional Chinese medicines, in particular to a method for constructing a standard decoction fingerprint of a five-finger wild peach decoction piece. Background Ficus HIRTA VAHL is dry root of Ficus aculeata of Moraceae, has sweet taste, and has effects of invigorating spleen, invigorating qi, promoting circulation of qi, removing dampness, relieving rigidity of muscles and activating collaterals. The five-finger wild peach is a common medicine and food homologous Chinese medicine in the Ling nan area, and has been accepted by the 1977 edition of Chinese pharmacopoeia. Modern researches have shown that Ficus simplicissima lour mainly contains various chemical components such as phenylpropanoids, flavonoids, terpenes, sterols, volatile oils, etc. In addition, psoralen has good testability, can enter blood in prototype, has liver protecting effect, and the anti-aging and anti-inflammatory effects of bergapten are consistent with the pharmacological effects of Ficus hirta. The standard decoction of traditional Chinese medicine decoction pieces is based on the guidance and clinical application of the traditional Chinese medicine theory, and is prepared by referring to the modern extraction method and the standardized process, and can be used for marking clinical medication, so that the accuracy of medication and the consistency of dosage are ensured. In view of the fact that research on the aspect of the standard decoction of the Ficus simplicissima lour decoction pieces at present has not been reported, the invention refers to relevant contents such as "treatment control and standard formulation technical requirement of traditional Chinese medicine formula particles" (the invention is called as technical requirement for short) "," management standard of traditional Chinese medicine decoction room of medical institution "(the invention is called as management standard for short)", and "research strategy of the standard decoction of the traditional Chinese medicine decoction pieces", etc., prepares the standard decoction of the Ficus simplicissima lour decoction pieces, establishes the quality standard thereof, and provides reference and basis for quality research and evaluation of the Ficus simplicissima lour formula particles and related preparations thereof. Disclosure of Invention The invention aims to provide a construction method of a Ficus simplicissima lour decoction piece standard decoction fingerprint spectrum, which aims to solve the problems in the prior art, and the construction method can be used for accurately constructing the Ficus simplicissima lour decoction piece standard decoction fingerprint spectrum, so that references and bases are provided for overall quality control and evaluation of Ficus simplicissima lour decoction piece standard decoction and formula particles. In order to achieve the above object, the present invention provides the following solutions: the invention provides a method for constructing a standard decoction fingerprint of Ficus simplicissima lour decoction pieces, which comprises the following steps: Adding psoralen and bergapten into 50% methanol water solution to obtain reference solution; Respectively extracting the five-finger nectarines in batches by water to obtain standard decoction in different batches, respectively adding methanol into the standard decoction in different batches until the volume fraction of the methanol is 50%, and centrifuging to obtain supernatant to obtain sample solutions in different batches; And respectively carrying out high performance liquid chromatography detection on the sample solutions in different batches to obtain fingerprint patterns, and calibrating characteristic peaks by using the reference solution. Further, the preparation method of the standard decoction comprises the steps of taking Ficus simplicissima lour decoction pieces, adding 8 times of water into the decoction pieces for soaking for 30min, boiling and decocting for 30min, filtering to obtain a first filtrate and filter residues, adding 6 times of water into the filter residues, boiling and decocting for 20min, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, and concentrating under reduced pressure to obtain the standard decoction. Further, the high performance liquid chromatography detection adoptsXB-C18 chromatographic column. Further, the mobile phase detected by the high performance liquid chromatography is methanol-0.1% phosphoric acid aqueous solution, wherein methanol is A phase, and 0.1% phosphoric acid aqueous solution is B phase. Further, the gradient elution procedure for high performance liquid chromatography detection is as follows: 0-10 min,30% of phase A; 10-30 min, 30-50% of phase A; 30-55 min, 50-95% of phase A; 55-60 min,95% phase A. Further, the