CN-121995002-A - Device and method for determining distribution of plant carbon assimilation source library

CN121995002ACN 121995002 ACN121995002 ACN 121995002ACN-121995002-A

Abstract

The invention relates to the technical field of isotope labeling, and provides a device and a method for measuring distribution of a plant carbon assimilation source library. The device for determining plant carbon assimilation source library distribution comprises a generator, a leaf chamber, a concentration monitor and a main controller, wherein the leaf chamber is connected with the generator, the concentration monitor is connected with the leaf chamber, the main controller is used for establishing a standard curve between the concentration of CO 2 and 13 CO 2 by combining the mass fraction of a solution containing 13 CO 3 in the generator according to CO 2 concentration data detected by the concentration monitor, acquiring the current mass fraction of 13 CO 2 according to the real-time detected concentration of CO 2 through the standard curve, and calculating to obtain a concentration value of 13 CO 2 based on the concentration of CO 2 and the mass fraction of 13 CO 2 . The device for measuring the distribution of the plant carbon assimilation source library realizes high-precision and low-cost control of 13 CO 2 marked gas concentration under the condition of not depending on an expensive isotope analyzer.

Inventors

CHEN XIN
HUANG SHITANG
HU JIARONG
SHI HAO
ZHU YUXIAN

Assignees

北京大学

Dates

Publication Date: 20260508
Application Date: 20251222

Claims (10)

1. An apparatus for determining the distribution of a plant carbon assimilation source library comprising: A generator for generating CO 2 , the CO 2 comprising 12 CO 2 and 13 CO 2 ; At least one assimilation unit, each of the assimilation units forming a circulation loop with the generator, each of the assimilation units comprising: The leaf chamber is provided with a cavity, plant tissues are contained in the cavity, and the leaf chamber is connected with the generator; A concentration monitor connected to the leaf chamber, the concentration monitor for monitoring the concentration of She Shina CO 2 ; The main controller is electrically connected with the concentration monitor and is used for establishing a standard curve between the concentration of CO 2 and the mass fraction 13 CO 2 according to the concentration data of the CO 2 detected by the concentration monitor and the mass fraction of the solution containing 13 CO 3 in the generator, acquiring the current mass fraction of 13 CO 2 according to the concentration of the CO 2 detected in real time through the standard curve, and calculating to obtain a concentration value of 13 CO 2 based on the concentration of the CO 2 and the mass fraction of 13 CO 2 .
2. The apparatus for determining plant carbon assimilation source library partitioning according to claim 1, wherein the leaf chamber comprises: the leaf chamber body is provided with the cavity, the leaf chamber body is provided with a first plate body and a second plate body which are opposite to each other, the first plate body is provided with a first groove, the second plate body is provided with a plurality of pairs of air inlet holes and air outlet holes, the generator is connected with the pair of air inlet holes and the pair of air outlet holes, and the concentration monitor is connected with the pair of air inlet holes and the pair of air outlet holes; The cover body is covered on the leaf chamber main body and is in sealing connection with the leaf chamber main body, the cover body is the printing opacity lid, the cover body is equipped with first logical groove and second logical groove, first logical groove with first recess intercommunication, first recess is used for holding the petiole of plant, the cavity is used for holding the blade of plant, first recess first logical groove with the second logical groove is sealed by flexible sealant.
3. The apparatus for determining plant carbon assimilation source library dispensing according to claim 2, wherein the leaf chamber further comprises an elastic member disposed in the first groove, the elastic member being provided with a second groove for receiving a petiole of a plant.
4. The apparatus for determining plant carbon assimilation source library dispensing according to claim 3, wherein a sealant is provided in the second groove.
5. The apparatus for determining plant carbon assimilation source library dispensing according to claim 2, wherein the leaf chamber further comprises a flexible sealing membrane, the bottom plate of the leaf chamber body is provided with a third through-slot, and the flexible sealing membrane is provided at the third through-slot.
6. The apparatus for determining plant carbon assimilation source library allocation according to claim 1, wherein the assimilation unit further comprises a flow pump provided to the circulation loop, the flow pump for adjusting a flow rate of the circulation gas in the circulation loop, the flow pump being electrically connected to the main controller.
7. The apparatus for determining plant carbon assimilation source library allocation according to claim 1, wherein the assimilation unit further comprises a barometer provided to the circulation circuit, the barometer being for detecting a barometric pressure of the circulation circuit.
8. The apparatus for determining plant carbon assimilation source library allocation according to claim 1, wherein the assimilation unit further comprises a buffer controller provided to the circulation loop, the buffer controller for reducing a difference between the leaf indoor environment parameter and the external environment parameter, the buffer controller being electrically connected to the main controller.
9. A method of determining a plant carbon assimilation source library dispensing device according to any of claims 1-8, comprising: Establishing a standard curve between the concentration of CO 2 and the mass fraction of 13 CO 2 ; Obtaining a first mass fraction of carbon in the source organ dry matter and a first isotope percentage of 13 C, and obtaining a second mass fraction of carbon in the sink organ dry matter and a second isotope percentage of 13 C; Respectively placing a source organ and a dry organ into the leaf chamber for photosynthesis, obtaining the concentration of She Shina CO 2 , and controlling the concentration of 13 CO 2 within a set range by using the standard curve based on the concentration of CO 2 ; obtaining a third mass fraction of carbon and a third isotope percentage of 13 C in the labeled source organ dry matter, a fourth mass fraction of carbon and a fourth isotope percentage of 13 C in the library organ dry matter at different time points; Calculating a carbon assimilation distribution efficiency of the source organ based on the first mass fraction, the first isotope percentage, the third mass fraction, and the third isotope percentage; Calculating a carbon assimilation distribution efficiency of the library organ based on the second mass fraction, the second isotope percentage, the fourth mass fraction, and the fourth isotope percentage.
10. The method of claim 9, wherein the step of establishing a standard curve between CO 2 concentration and 13 CO 2 mass fraction comprises: Maintaining the total mass of the carbon isotope salt solution in the generator constant, changing the mass fraction of the solution containing 13 CO 3 , and obtaining the concentration of CO 2 generated by the solution containing 13 CO 3 at different mass fractions; Obtaining a mass fraction of 13 CO 2 based on the principle that 13 CO 2 generated by the reaction is equal to the mass fraction of the solution containing 13 CO 3 ; And performing curve fitting by taking the mass fraction of 13 CO 2 as an abscissa and the concentration of CO 2 as an ordinate to obtain a standard curve between the concentration of CO 2 and the mass fraction of 13 CO 2 .

Description

Device and method for determining distribution of plant carbon assimilation source library Technical Field The invention relates to the technical field of isotope labeling, in particular to a device and a method for measuring distribution of a plant carbon assimilation source library. Background In plant physiology and metabolism studies, stable isotope labeling techniques (e.g., 13C-CO2 labeling) are key tools for resolving carbon assimilation product transport and partitioning mechanisms. For economic crops such as cotton, the cotton boll alignment leaves are used as core photosynthetic source organs, and the photosynthetic efficiency and the transfer capacity of assimilates to cotton bolls directly determine the quality and yield of cotton fibers. Currently, a variety of leaf chamber systems have been used for plant photosynthetic assays and isotope labeling. For example, evans et al designed a dynamic flow leaf chamber with temperature control, airflow uniformity and rapid gas switching functions, mainly for Arabidopsis research, K lling et al developed a 14CO2 marker for single leaf, Ć erani ć et al reported a method for achieving uniform 13 C and 15 N marking of whole plants using custom growth chambers. However, the above-described systems rely on external gas sources or simple reactions, lack effective control over gas concentration, flow and distribution, marking efficiency is low and detection equipment is expensive, resulting in higher cost for the leaf chamber system. The prior art lacks a system which can realize low cost and high precision 13CO2 concentration control, is applicable to various plant organs and supports high-flux parallel experiments. Disclosure of Invention The invention provides a device and a method for determining distribution of a plant carbon assimilation source library, which are used for solving the defects of low marking efficiency and expensive detection equipment in the prior art. The invention provides a device for determining plant carbon assimilation source library distribution, which comprises a generator, at least one assimilation unit, a concentration monitor, a main controller and a concentration controller, wherein the generator is used for generating CO 2, the CO 2 comprises 12CO2 and 13CO2, the assimilation unit and the generator form a circulation loop, each assimilation unit comprises a leaf chamber, the leaf chamber is provided with a cavity, plant tissues are contained in the cavity, the leaf chamber is connected with the generator, the concentration monitor is connected with the leaf chamber and is used for monitoring the concentration of She Shina CO 2, the main controller is electrically connected with the concentration monitor and is used for establishing a standard curve between the concentration of CO 2 and the mass fraction 13CO2 according to the concentration data of CO 2 detected by the concentration monitor and the mass fraction of 13CO3 -containing solution in the generator, the mass fraction of current 13CO2 is obtained through the standard curve according to the concentration of the CO 2 detected in real time, and the concentration value of the concentration of the CO 56 13CO2 is calculated and obtained based on the concentration of the CO 2 and the mass fraction of the 2. The device for determining distribution of plant carbon assimilation source libraries comprises a leaf chamber main body, a cover body, a first through groove and a second through groove, wherein the leaf chamber main body is provided with a first plate body and a second plate body which are opposite to each other, the first plate body is provided with a first groove, the second plate body is provided with a plurality of pairs of air inlet holes and air outlet holes, the generator is connected with the pair of air inlet holes and the pair of air outlet holes, the concentration monitor is connected with the pair of air inlet holes and the pair of air outlet holes, the cover body is covered on the leaf chamber main body and is in sealing connection with the leaf chamber main body, the cover body is a light-transmitting cover body, the cover body is provided with the first through groove and the second through groove, the first through groove is communicated with the first groove, the first groove is used for accommodating plant leaf handles, and the cavity is used for accommodating plant leaves, and the first groove, the first through groove and the second through groove are sealed by flexible sealant. According to the device for determining distribution of the plant carbon assimilation source library, provided by the invention, the leaf chamber further comprises an elastic piece, the elastic piece is arranged in the first groove, the elastic piece is provided with a second groove, and the second groove is used for accommodating a leaf stalk of a plant. According to the device for determining distribution of the plant carbon assimilation source library, provided by the inven