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CN-121995012-A - Method for screening tobacco varieties with high flavonoid content based on Multiplex 3

CN121995012ACN 121995012 ACN121995012 ACN 121995012ACN-121995012-A

Abstract

The invention relates to the technical field of tobacco agriculture, in particular to a method for screening tobacco varieties with high flavonoid content based on Multiplex 3, which is used for screening tobacco varieties with high flavonoid content tobacco leaves by detecting the flavonoid content of tobacco leaves through Multiplex 3. Compared with the prior art, the method provided by the invention has the advantages of simplicity, rapidness, accuracy, no damage, low destructiveness, safety and reliability, and is not limited by application scenes.

Inventors

  • SUN XIAOWEI
  • HE MENGYING
  • Guo Daowei
  • WANG JIALONG
  • GUO WENMENG

Assignees

  • 河南省烟草公司许昌市公司

Dates

Publication Date
20260508
Application Date
20260309

Claims (8)

  1. 1. A method for non-destructive rapid screening of high flavonoid content tobacco based on Multiplex 3, said method comprising the steps of: under the same environmental parameters and sampling conditions, using a Multiplex 3 sensor to determine the flavonoid contents of the control tobacco sample leaves and the experimental tobacco sample leaves, and obtaining a screening threshold according to the flavonoid content results of the control tobacco sample leaves; According to the screening threshold and the flavonoid content of the experimental tobacco sample leaves, primarily screening candidate tobacco single plants with high flavonoid content; and under the differential condition of changing at least one of the environmental parameters or sampling conditions, re-determining the flavonoid content of the candidate tobacco individual plant leaves by using a Multiplex 3 sensor, wherein under the differential condition, the tobacco with the flavonoid content still higher than the screening threshold value is the tobacco variety with high flavonoid content.
  2. 2. The method of claim 1, wherein the environmental parameters and sampling conditions comprise three of: 1) Selecting a period of time with the illumination intensity of 300 lux-500 lux for measurement; 2) Selecting tobacco leaves with the same growth period and the same leaf position; 3) The measurement was performed in the same position area on the leaf that avoided the vein.
  3. 3. The method of claim 1, wherein the leaf area of the tobacco sample leaf is greater than or equal to 28cm 2 , wherein if the leaf area of the tobacco sample leaf is less than 28cm 2 , a black material is used to shield the light source, and wherein the area of the black material is an area that complements the difference between the leaf area of the tobacco sample leaf and 28cm 2 .
  4. 4. The method of claim 1, wherein the leaf is a fresh leaf.
  5. 5. The method according to claim 1, wherein the setting of the screening threshold comprises the steps of: carrying out normal distribution test on the measured flavonoid content value, and drawing a measured value-density curve; Calculating the mean mu and standard deviation sigma of the control group; the screening threshold is set to a value interval in the range of μ+σ to μ+2σ.
  6. 6. The method of claim 1, wherein the differentiation conditions comprise three of: 1) Selecting a measurement time different from the preliminary screening; 2) Selecting tobacco leaf positions different from the preliminary screening; 3) The measured positions on tobacco leaves different from the preliminary screening were selected.
  7. 7. The method of claim 1, wherein each sample is measured at least 3 consecutive times using a Multiplex 3 sensor.
  8. 8. The method of claim 1, wherein the Multiplex 3 sensor is a Multiplex 3 mx315411 model Force-a.

Description

Method for screening tobacco varieties with high flavonoid content based on Multiplex 3 Technical Field The invention belongs to the technical field of tobacco agriculture, and particularly relates to a method for screening tobacco varieties with high flavonoid content based on Multiplex 3. Background Flavonoid compounds are secondary metabolites widely distributed in plants, and more than 10000 compounds are reported, so that the flavonoid compounds have important pharmacological activities such as anticancer and anti-inflammatory. The stress-resistant effects of flavonoids in plants are of great interest and their role in regulating polar auxin transport and free radical scavenging mechanisms is emphasized. Plants under stress accumulate flavonoids by modulating expression of the flavonoid synthase gene, and flavonoid compounds modulate Polar Auxin Transport (PAT) in the form of ATP-binding cassette subfamily B/P-glycoprotein (ABCB/PGP) transporters by acting on the auxin export vector PIN-FORMED (PIN). Phenylpropanoids and flavonoids play a vital role in plant defense against biotic and abiotic stresses. They can mediate defensive responses such as accumulation in leaves and glandular hairs and are associated with plant resistance to fungi and oomycetes. Therefore, plant flavonoids play an important role in biosynthesis, biological function and in plant growth and development and environmental stress response. Flavonoid compounds are one of important products in the secondary metabolism process of tobacco, play an important role in the growth and development of tobacco, and are an important factor for measuring the quality of tobacco. Accumulation, conversion and degradation of the products in the processes of tobacco maturation, modulation, alcoholization and combustion generate dihydric phenols and furfural derivatives, and the products have direct influence on the flavor of the flue gas, can endow the flue gas with sweet flavor and roasted flavor, and can directly influence the flavor style, the flavor quality and the flavor quantity of flue-cured tobacco. Flavonoid compounds and polysaccharide extracts in tobacco leaves show good antioxidant activity in vitro antioxidant property research, and flavonoid compounds show better activity than polysaccharide in terms of scavenging free radicals, so that the flavonoid compounds and polysaccharide extracts have the potential of becoming powerful antioxidants. In addition, flavonoid substances also show improved resistance to various diseases in researches on disease resistance in tobacco, such as ralstonia solanacearum, colletotrichum gloeosporioides and Alternaria tabacum. At present, the flavonoid content in tobacco leaves is mostly obtained through field sampling and laboratory chemical analysis, if the sampling amount is small, the flavonoid content level of field tobacco cannot be truly reflected, and large-scale monitoring is difficult to realize, and if the sampling amount is enlarged, the accuracy is improved, but the operation is complex, the timeliness is poor, a large number of samples need to be acquired destructively, and the cost is high, so that the development of a method which is simple, quick, high in accuracy, low in destructiveness and suitable for field analysis is very necessary. Disclosure of Invention In order to solve the problems, the invention provides a method for screening tobacco varieties with high flavonoid content based on Multiplex 3, which has the advantages of simplicity, rapidness, accuracy, no damage, low destructiveness and no limitation of application scenes, can realize rapid and instant monitoring of the flavonoid content in the field, and provides theoretical basis and technical support for monitoring the flavonoid content in the field, managing the field of tobacco and tobacco agriculture technology. In order to achieve the above purpose, the specific technical scheme of the invention is as follows: In a first aspect, the present invention provides a method for screening tobacco varieties with high flavonoid content based on Multiplex 3, said method comprising the steps of: under the same environmental parameters and sampling conditions, using a Multiplex 3 sensor to determine the flavonoid contents of the control tobacco sample leaves and the experimental tobacco sample leaves, and obtaining a screening threshold according to the flavonoid content results of the control tobacco sample leaves; According to the screening threshold and the flavonoid content of the experimental tobacco sample leaves, primarily screening candidate tobacco single plants with high flavonoid content; and under the differential condition of changing at least one of the environmental parameters or sampling conditions, re-determining the flavonoid content of the candidate tobacco individual plant leaves by using a Multiplex 3 sensor, wherein under the differential condition, the tobacco with the flavonoid content still higher than the scree