CN-121995719-A - Dual-band Shan Xiangsu holographic microscopic imaging device

CN121995719ACN 121995719 ACN121995719 ACN 121995719ACN-121995719-A

Abstract

The invention relates to the technical field of computational imaging and microscopic imaging. A dual-band Shan Xiangsu holographic microscopic imaging device uses invisible light band to perform penetrating imaging on a medium or thick tissue sample with scattering property, and simultaneously uses visible light band to obtain high-resolution surface information of the sample to realize dual-band complementary observation, the device comprises a dual-band illumination module composed of a visible laser (1), an invisible laser (2) and a dichroic mirror (3), the device comprises a beam combination and expansion module consisting of a collimation beam expander (4) and a condenser (5), an objective lens (6), a sample to be tested, an off-axis parabolic lens group consisting of a first off-axis parabolic lens (7), a second off-axis parabolic lens (8) and a third off-axis parabolic lens (9), a space modulation element (10), a fourth off-axis parabolic lens (11) and a fifth off-axis parabolic lens (12).

Inventors

WANG DONG
Lan xuan
ZHENG TINGTING
ZHAO WENJING
ZHAI AIPING

Assignees

太原理工大学

Dates

Publication Date: 20260508
Application Date: 20260205

Claims (7)

1. A dual-band Shan Xiangsu holographic microscopic imaging device is characterized in that the device utilizes an invisible light band to carry out penetrating imaging on a medium or a thick tissue sample with scattering characteristics, and simultaneously utilizes a visible light band to acquire high-resolution surface form information of the sample to realize dual-band complementary observation, the device comprises a dual-band illumination module consisting of a visible laser (1), an invisible light laser (2) and a dichroic mirror (3), a beam combining and expanding module consisting of a collimating and beam expander (4) and a condenser (5), an objective lens (6), a sample to be detected, an off-axis parabolic lens group consisting of a first off-axis parabolic mirror (7), a second off-axis parabolic mirror (8) and a third off-axis parabolic mirror (9), a spatial modulation element (10), a fourth off-axis parabolic mirror (11), a fifth off-axis parabolic mirror (12), a detector group consisting of a first pinhole (13) and a second pinhole (14), a detector group consisting of a first detector (15) and a second detector (16), a dichroic mirror (3) and a laser beam (1) and a second mirror (4) which emit coherent light beam to be detected to the same as the laser beam to be detected and the laser beam to be detected (4) after passing through the same optical axis of the first off-axis parabolic mirror (7) and the second off-axis parabolic mirror (9), and then, transmitting the wave front of the sample to a space modulation element (10) through a second off-axis parabolic mirror (8) and a third off-axis parabolic mirror (9) of a 4f system with a relay function, loading a modulation mask on the space modulation element (10) to modulate a light beam, carrying out Fourier transform on the modulated light path after the space modulation element (10) is modulated through a fourth off-axis parabolic mirror (11) and a fifth off-axis parabolic mirror (12), and then, receiving and filtering visible light and invisible light signal data of non-zero diffraction order components through a pinhole group and a detector group, and combining the obtained data with a reconstruction algorithm through an interference method to obtain an imaging result.
2. The dual-band Shan Xiangsu holographic microscopy imaging device of claim 1, wherein the invisible light band is infrared band or ultraviolet band.
3. The dual-band Shan Xiangsu holographic microimaging device as in claim 1, wherein the spatial modulation element is any one of a digital micromirror device, a spatial light modulator, or a magneto-optical modulator, and the wavelength ranges of the visible light band and the invisible light band are within the operating wavelength response range of the spatial modulation element.
4. A dual band Shan Xiangsu holographic microscopy imaging means as defined in claim 1, wherein the off-axis parabolic mirror set, fourth off-axis parabolic mirror (11) and fifth off-axis parabolic mirror (12) are selected from off-axis parabolic mirrors.
5. The dual-band Shan Xiangsu holographic microscopy imaging device of claim 1, wherein the objective lens is a transmissive objective lens, a reflective objective lens or a catadioptric hybrid objective lens.
6. The dual-band Shan Xiangsu holographic microscopy imaging device of claim 1, wherein the interference is an off-axis interference or a phase-shift interference.
7. The dual-band Shan Xiangsu holographic microscopy imaging of claim 1, wherein the reconstruction algorithm is TVAL or a second order correlation algorithm.

Description

Dual-band Shan Xiangsu holographic microscopic imaging device Technical Field The invention relates to the technical field of computational imaging and microscopic imaging. Background The holographic microscope imaging technology can simultaneously acquire the amplitude and phase information of the microscale sample, and plays a vital role in the fields of biomedical research and material science. However, conventional holographic microscopes typically rely on detection by cameras, due to the high cost and complexity of high performance infrared detection techniques, and limited infrared detection distances. In contrast, single-pixel microscopes have become a novel computational imaging modality that skillfully achieves indirect acquisition of optical field image information by a combination of measurement and computation, whereby the advantages of low cost, broad spectral response, and high sensitivity are utilized to represent color in the infrared band. Despite the development of single-pixel microscopy, integration of long-wave and short-wave holographic imaging in the same set of microscopy systems and their complementary advantages have not been achieved. Therefore, a single-pixel holographic microscopic imaging system which can simultaneously utilize the strong penetrability of long waves and the high resolution of short waves and has the phase reconstruction capability is developed, and the single-pixel holographic microscopic imaging system has important application value for the research of complex biological samples. Disclosure of Invention The invention aims to solve the technical problem of providing a single-pixel holographic microscopic imaging device which can simultaneously utilize high penetrability of long waves and high resolution of short waves, can complementarily observe and has phase reconstruction capability. The technical scheme adopted by the invention is that the device comprises a dual-band Shan Xiangsu holographic microscopic imaging device, a dual-band illuminating module, an objective lens (6), a sample to be detected, an off-axis parabolic lens group, a space modulation element (10), a fourth off-axis parabolic lens (11), a fifth off-axis parabolic lens (12), a pinhole group, a detector group, and a first detector (15) and a second detector (16), wherein the dual-band illuminating module consists of a visible laser (1), an invisible laser (2) and a dichroic mirror (3), the beam combining and expanding module consists of a collimating and beam expander (4) and a condenser (5), the objective lens (6) is used for carrying out penetrating imaging on a medium or thick tissue sample with scattering characteristics by utilizing an invisible light band, the off-axis parabolic lens group consists of a first off-axis parabolic lens (7), a second off-axis parabolic lens (8) and a third off-axis parabolic lens (9), the space modulation element (10), the fourth off-axis parabolic lens (11), the fifth off-axis parabolic lens (12), the detector group consists of a first pinhole (13) and a second pinhole (14), the detector group consists of a first detector (15) and a second detector (16), the detector (3) is used for combining the visible light beam (1) and the second laser beam (4) to the same as the optical beam after the optical beam passes through the optical axis parabolic lens (7) and the second parabolic lens (5) and the optical lens (7) to be focused, and then, transmitting the wave front of the sample to a space modulation element (10) through a second off-axis parabolic mirror (8) and a third off-axis parabolic mirror (9) of a 4f system with a relay function, loading a modulation mask on the space modulation element (10) to modulate a light beam, carrying out Fourier transform on the modulated light path after the space modulation element (10) is modulated through a fourth off-axis parabolic mirror (11) and a fifth off-axis parabolic mirror (12), and then, receiving and filtering visible light and invisible light signal data of non-zero diffraction order components through a pinhole group and a detector group, and combining the obtained data with a reconstruction algorithm through an interference method to obtain an imaging result. The invisible light wave band is an infrared wave band or an ultraviolet wave band. The spatial modulation element is any one of a digital micromirror device, a liquid crystal spatial light modulator or a magneto-optical modulator, and the wavelength ranges of the visible light wave band and the invisible light wave band are within the working wavelength response range of the spatial modulation element. The off-axis parabolic mirror group, the fourth off-axis parabolic mirror (11) and the fifth off-axis parabolic mirror (12) are selected from off-axis parabolic mirrors. The objective lens adopts a transmission type objective lens, a reflection type objective lens or a catadioptric hybrid type objective lens. The method of interference is off-axis inter