CN-121996867-A - Radix puerariae quality evaluation method by combining multi-index quantification with entropy weight method-TOPSIS method

CN121996867ACN 121996867 ACN121996867 ACN 121996867ACN-121996867-A

Abstract

The invention relates to a kudzuvine root quality evaluation method by a multi-index quantitative combination entropy weight method-TOPSIS method, which comprises the steps of crushing kudzuvine root raw materials, optimizing parameters such as an extraction method, an extraction solvent, extraction time and the like, adopting the optimal extraction process to obtain a kudzuvine root extract, detecting compounds, adopting an ultra-high performance liquid chromatography-high resolution mass spectrometer, optimizing mass spectrum and chromatographic parameters to realize qualitative and quantitative analysis, and determining the weight of each quality evaluation index by an entropy weight method by taking detected compound content data as quality evaluation indexes, and calculating the distance from each quality evaluation index to an optimal scheme and the distance from each quality evaluation index to a worst scheme based on the TOPSIS method to obtain the relative proximity degree between kudzuvine roots of different production places and the optimal scheme as the basis of quality evaluation. The quality evaluation of the kudzuvine root in different producing areas is realized by applying the method, the quality evaluation index data information of each quality evaluation index is fully utilized, the quality difference of different kudzuvine roots is accurately reflected, and the influence of subjective factors is avoided.

Inventors

YIN YANYAN
FAN QIGAO
ZHANG MENGRUI
LI LULU
WANG YEMEI
LU HU

Assignees

贵州习酒股份有限公司

Dates

Publication Date: 20260508
Application Date: 20251120

Claims (10)

1. A method for evaluating quality of radix puerariae is characterized in that the quality of radix puerariae is evaluated according to physical and chemical indexes of radix puerariae, wherein the physical and chemical indexes of radix puerariae comprise puerarin content, daidzin content, daidzein content, genistin content and luteolin content.
2. The evaluation method according to claim 1, wherein the physicochemical index of kudzuvine root further comprises a rutin content; preferably, when the kudzuvine root is a powdery kudzuvine root, the physicochemical index of the kudzuvine root is selected from the group consisting of the content of puerarin, the content of daidzein, the content of genistin and the content of luteolin; Preferably, when the kudzuvine root is the root of kudzu vine, the physicochemical index of the kudzuvine root is selected from the group consisting of puerarin content, daidzein content, genistin content, luteolin content and rutin content.
3. The evaluation method according to claim 1 or 2, wherein the evaluation result of the quality of kudzuvine root is obtained by combining the physicochemical index of kudzuvine root by an entropy weight method-TOPSIS method.
4. A method of evaluating according to any one of claims 1 to 3, wherein the method of evaluating comprises the steps of: (a) Obtaining physical and chemical indexes of the kudzuvine root; (b) Constructing an original data matrix X based on the physicochemical indexes of the kudzuvine root obtained in the step (a), carrying out normalization processing on the original data matrix, and constructing a normalized quality evaluation matrix Y; (c) Calculating the weight of the physicochemical index of each kudzuvine root in the normalized quality evaluation matrix according to an entropy weight method based on the normalized quality evaluation matrix Y obtained in the step (b); (d) Based on the weight of the physicochemical index of the kudzuvine root obtained in the step (c), analyzing the quality of the kudzuvine root by adopting a TOPSIS method; preferably, the physicochemical indexes of the kudzuvine root comprise puerarin content, daidzin content, daidzein content, genistin content and luteolin content; preferably, the physicochemical index of the kudzuvine root further comprises the content of rutin; preferably, when the kudzuvine root is a powdery kudzuvine root, the physicochemical index of the kudzuvine root is selected from the group consisting of the content of puerarin, the content of daidzein, the content of genistin and the content of luteolin; Preferably, when the kudzuvine root is the root of kudzu vine, the physicochemical index of the kudzuvine root is selected from the group consisting of puerarin content, daidzein content, genistin content, luteolin content and rutin content.
5. The method of evaluating according to claim 4, wherein the method of obtaining physical and chemical indicators of kudzuvine root in step (a) comprises the steps of: (1) Reflux extracting radix Puerariae in solvent; (2) Centrifuging to obtain supernatant; (3) Diluting the supernatant, and filtering to obtain a liquid to be detected; (4) Performing qualitative and quantitative analysis on the liquid to be detected by utilizing high performance liquid chromatography-mass spectrometry to obtain physical and chemical indexes of the radix puerariae; Preferably, the temperature of the reflux extraction in the step (1) is 60-90 ℃; preferably, the reflux extraction time in the step (1) is 40-70 min; preferably, the solvent in the step (1) comprises ethanol, methanol, n-butanol, chloroform, acetone, ethyl acetate or water; Preferably, the volume fraction of the solvent in the step (1) is 40% -100%; Preferably, in the step (1), the mass/volume ratio of the kudzuvine root to the solvent is 1:20-1:40; preferably, the centrifugation condition in the step (2) is 5000-10000 rpm for 4-20 min; Preferably, the filtering in the step (3) comprises treatment with an organic filter membrane of 0.22-0.45 μm; Preferably, the conditions of the high performance liquid chromatography in the step (4) comprise a chromatographic column of a Hypersil GOLD chromatographic column (150×2.1 mm,1.9 μm), a column temperature of 25-35 ℃, a sample injection volume of 1-5 μl, a flow rate of 0.1-0.3 mL/min, and a mobile phase comprising an aqueous phase of 0.05% -0.15% formic acid aqueous solution (a) and an organic phase of methanol (B); Preferably, the gradient elution procedure of the high performance liquid chromatography is as follows: 0.0 min to 1.0 min, a flow rate of 0.2 to mL/min, a mobile phase A of 95% formic acid aqueous solution (V/V), a mobile phase B of 5% methanol (V/V), 1.0 min to 5.0 min, a flow rate of 0.2 to mL/min, a mobile phase A of 5% formic acid aqueous solution (V/V), a mobile phase B of 95% methanol (V/V), 5.0 to 8.0 min, a flow rate of 0.2 to mL/min, a mobile phase A of 5% formic acid aqueous solution (V/V), a mobile phase B of 95% methanol (V/V), 8.0 to min to 11.0 min, a flow rate of 0.2 to mL/min, a mobile phase A of 95% formic acid aqueous solution (V/V), a mobile phase B of 5% methanol (V/V), a flow rate of 11.0 min~12.0 min, a flow rate of 0.2 to mL/min, a mobile phase A of 95% formic acid aqueous solution (V/V), and a mobile phase B of 5% methanol (V/V); Preferably, the conditions of the mass spectrum comprise an electrospray ionization positive/negative ion switching ion mode, a spray voltage of 3.0-4.0 kV, a sheath gas of 30-40 psi, an auxiliary gas of 1-10 arb, an ion source heating temperature of 250-350 ℃, an ion transmission tube temperature of 330-370 ℃, a scanning mode of Full MS/dd-MS 2 , a scanning range of 100 m/z-800 m/z, a primary scanning resolution of 50000-90000 and a secondary scanning resolution of 20000-60000.
6. The method of claim 4, wherein the normalized quality evaluation matrix Y of step (b) is obtained by the formula (1): (1); the method for obtaining the weight of the physical and chemical indexes of the kudzuvine root in the step (c) comprises the following steps: based on the normalized quality evaluation matrix Y, calculating the information entropy Ej of the physicochemical indexes of the kudzuvine root by the formula (2): (2); Based on the information entropy of the physicochemical indexes of the kudzuvine roots, calculating the weight Wj of the physicochemical indexes of the kudzuvine roots by the formula (3): (3); Wherein X is an original data matrix, xij is represented as a j-th physicochemical index of kudzuvine root of an i-th production place, i=1, 2,3, n, j=1, 2,3, m, n is the total number of samples of kudzuvine root, m is the total number of physicochemical indexes, max (Xij) is the maximum value of the j-th physicochemical index in kudzuvine root of all production places in the original data matrix, and min (Xij) is the minimum value of the j-th physicochemical index in kudzuvine root of all production places in the original data matrix.
7. The method of evaluating as claimed in claim 4, wherein the analysis of the quality of kudzuvine root by using TOPSIS method in the step (d) comprises the steps of: Obtaining a weighting standard matrix V according to a formula (4): (4); According to the weighting standard matrix V, determining a theoretical optimal scheme value Vj + in the radix puerariae samples of all the producing places and a theoretical worst scheme value Vj - in the radix puerariae samples of all the producing places; Based on the theoretical optimal solution value Vj + in the radix puerariae samples of all producing places and the theoretical worst solution value Vj - in the radix puerariae samples of all producing places, respectively calculating the distance D + i between the physicochemical indexes of the radix puerariae in each producing place and the optimal solution, the distance D - i between the physicochemical indexes and the worst solution and the European closeness Ci of the optimal solution according to the formula (5): (5); wherein Z is a standardized matrix, zij is a standard value of the j-th physical and chemical index of the i-th production place, V is a weighted standardized matrix, vij is a weighted standard value of the j-th physical and chemical index of the i-th production place, and Ci is evaluated according to Ci, and the larger Ci is, the better Ci is.
8. An evaluation method for judging a root of kudzu vine origin, characterized in that an evaluation result of the root of kudzu vine origin is obtained according to the evaluation method of any one of claims 1 to 7.
9. An evaluation device for quality of kudzuvine root, comprising: The data acquisition module is used for acquiring physical and chemical index data of the radix puerariae sample; The data processing module is used for carrying out normalization processing on the physical and chemical index data of the kudzuvine root by combining an entropy weight method to obtain normalized physical and chemical index data of the kudzuvine root, and calculating the weight of the physical and chemical index data of each kudzuvine root; The evaluation module is used for calculating the relative proximity of the radix puerariae samples and outputting quality grade or sequencing result based on the normalized radix puerariae physicochemical index data and weight by combining a TOPSIS method; preferably, the physicochemical indexes of the kudzuvine root comprise puerarin content, daidzin content, daidzein content, genistin content and luteolin content; preferably, the physicochemical index of the kudzuvine root further comprises the content of rutin; Preferably, the physicochemical indexes of the kudzuvine root further comprise a combination of puerarin content, daidzin content, daidzein content, genistin content and luteolin content; Preferably, the physicochemical indexes of the kudzuvine root further comprise a combination of puerarin content, daidzin content, daidzein content, genistin content, luteolin content and rutin content; Preferably, the data acquisition module comprises a high performance liquid chromatograph and a mass spectrometer, and the data processing module comprises a computer program with built-in entropy weight method and TOPSIS method.
10. Use of the evaluation method according to any one of claims 1 to 8 or the evaluation device according to claim 9 for evaluating the quality of kudzuvine root.

Description

Radix puerariae quality evaluation method by combining multi-index quantification with entropy weight method-TOPSIS method Technical Field The invention relates to the field of traditional Chinese medicine detection, in particular to a kudzuvine root quality evaluation method by combining a multi-index quantitative entropy weight method with a TOPSIS method. Background The kudzuvine root is used as a traditional Chinese medicinal material, and is called as a thousand years ginseng because of rich nutrition and strong pharmacological action, and is listed in the article list of foods and medicines in 2000. In the pharmacopoeia of 2020, pueraria lobata is divided into two varieties of Pueraria lobata (wild arrowroot) Ohwi and Pueraria lobata (wild arrowroot) Pueraria thomsonii Benth, wherein the Pueraria lobata is a wild variety and the Pueraria lobata is a cultivated variety. The data report shows that the chemical components of the two materials are basically similar, but the content level difference is larger. The kudzuvine root resources in China are rich, most provinces in China are distributed, but kudzuvine roots in different producing areas are affected by factors such as environment, climate and the like, the quality difference is large, and the quality is uneven. The main active ingredient in the kudzuvine root is flavonoid compound, has certain pharmacological activity in the aspects of resisting diabetes, treating alcoholic liver disease and the like, and has potential application value in the aspects of development of medicines and health-care products. Therefore, an efficient extraction process and an accurate quantification method of flavonoid compounds in the kudzuvine root are established, the content difference of main compounds in different kudzuvine roots is clarified, the quality control and evaluation of the kudzuvine roots are realized, and the improvement of the utilization value of kudzuvine root resources is facilitated. At present, the extraction process of flavonoid compounds in kudzuvine root is mainly divided into three categories, namely a physical extraction method, a chemical extraction method and a biological extraction method. The physical extraction method is the widest extraction method in actual production application at present because of low operation cost and safe and controllable process. Because of more flavonoid compounds in the kudzuvine root and different chemical structure properties, the requirements on the extraction process are high, and the extraction efficiency is greatly influenced by different extraction parameters. The flavonoid compound is used as a main active ingredient in the kudzuvine root, and the current detection method mainly comprises an ultraviolet spectrophotometry, a high performance liquid chromatography and a liquid chromatography tandem mass spectrometry. The ultraviolet spectrophotometry can only measure the total flavone content in the kudzuvine root, and can not realize qualitative and quantitative analysis of the flavone compounds. The high performance liquid chromatography uses the retention time of the target object to be detected and the standard substance as a qualitative basis and uses the peak area for quantification, but the pueraria is various in compound types and complex in matrix, so that the accuracy of the measurement result is low. The liquid chromatography tandem mass spectrometry is characterized in that the accurate mass number of the target quantitative ions is used for qualitative and quantitative, and the accuracy is high, but the analysis condition requirement is high because of various compound types in the kudzuvine root and the existence of isomer compounds. The current kudzuvine root quality evaluation method mainly comprises a conventional analysis technology for morphological subjective judgment, identification and chemical composition. The subjective judgment and identification of the morphology has low accuracy of the judgment result due to strong subjectivity. The conventional analysis technology of chemical components mainly carries out comparative analysis from the contents of total flavone, starch, cellulose and the like, but the quality level of the kudzuvine root cannot be intuitively reflected due to the abundant types of the compounds in the kudzuvine root, which is not beneficial to the utilization of kudzuvine root resources and the development of products. In the Chinese pharmacopoeia, the quality evaluation of the kudzuvine root only takes puerarin as a quality evaluation index, and the quality of the kudzuvine root cannot be objectively and comprehensively reflected, so that the development of an objective and accurate kudzuvine root quality evaluation method is urgently needed. Disclosure of Invention In some embodiments, the invention provides a method for evaluating quality of kudzuvine root, which evaluates the quality of kudzuvine root according to physical and chemical indexes of kudzuvin