CN-122000092-A - Network pharmacological target screening method for application of nano curcumin to plateau skin burn and wound composite injury and application of nano curcumin in preparation of medicament for treating plateau burn and wound composite injury

CN122000092ACN 122000092 ACN122000092 ACN 122000092ACN-122000092-A

Abstract

The invention discloses a network pharmacological target screening method of nano curcumin applied to plateau skin burn and wound composite wounds and application of the nano curcumin in preparation of medicaments for treating the plateau skin burn and wound composite wounds, and relates to the technical field of medicaments. Nano curcumin in the medicine plays a role in promoting recovery in early stage of wound surface by promoting wound surface healing of burn and wound composite injury; the medicine can relieve inflammatory reaction and oxidation reaction of organism by inhibiting AKT-IκB-NF κB signaling pathway; the nano curcumin can effectively reduce inflammatory reaction and oxidative stress injury of the wound surface by accurately regulating and controlling AKT/iκB/NF κB signal paths, and provides a new medical strategy for promoting healing of the burn-wound composite injury in a special highland environment.

Inventors

LIU JIANGWEI
ZHOU ENNING
SHI CHUANKAI
SHEN CAIFU
CHEN CHANGXU
Mao Zehai
SU LISONG
REN KEPENG

Assignees

中国人民解放军陆军第九五七医院

Dates

Publication Date: 20260508
Application Date: 20260224

Claims (10)

1. A network pharmacological target screening method for applying nano curcumin to plateau skin burn and wound compound injury is characterized by comprising the following steps: s1, obtaining a curcumin-related protein target; s2, obtaining a protein target spot related to the plateau burn wound; s3, constructing a protein interaction network and selecting a key target point; S4, cross target enrichment analysis, namely guiding the common target of the nano curcumin aiming at the burn wound into a metscape platform to obtain GO and KEGG enrichment data; S5, molecular docking, namely, receptor proteins are NF kappa B, AKT and I kappa B (PDB ID:1 SVC), a semi-flexible docking mode is set, a docking algorithm is a Ramark genetic algorithm, and Auto Dock Vina.1.2.5 software is operated to carry out molecular docking to obtain docking combination free energy and a docking result file; S6, molecular dynamics simulation, namely performing molecular dynamics simulation on the complex of the NF- κB protein and the curcumin by using software.
2. An application of nano curcumin obtained by adopting the network pharmacology target screening method of the nano curcumin applied to the plateau skin burn injury complex injury in preparing medicines for treating the plateau burn injury complex injury.
3. The method according to claim 2, wherein the nano curcumin is used for preparing a medicament for treating the composite wound of the plateau burn, and the dosage of the nano curcumin in the medicament is 30mg/ml-50mg/ml.
4. The application of the medicine according to claim 3, wherein the nano curcumin in the medicine is used for promoting recovery by promoting the wound healing of the burn and wound composite wound and plays a role in early stage of the wound, and the dosage of the nano curcumin in the medicine is 45mg/ml.
5. The method according to claim 2, wherein the nano curcumin is used for preparing a medicament for regulating an Akt-NF kappa B signal pathway, and the medicament can regulate an apoptosis signal pathway and oxidative stress.
6. The method according to claim 5, wherein the nano curcumin targets include NF- κ B, AKT and I- κB.
7. The application of the nano curcumin is characterized in that the nano curcumin, the NF- κB and the protein form multiple binding forces, the binding energy is smaller than-7.2 kcal mol -1 , and the core action target point of the nano curcumin in the medicament is the NF- κB.
8. The method of claim 5, wherein the agent reduces inflammatory and oxidative reactions in the body by inhibiting the AKT-IκB-NF κB signaling pathway.
9. The method of claim 2, wherein the agent is capable of reducing the levels of the pro-inflammatory cytokines IL-6, IL-1. Beta., TNF-alpha. And oxidative stress marker MDA induced by the composite trauma of the plateau burn.
10. The method according to claim 8, wherein the medicament comprises a therapeutically effective amount of nano-curcumin and a pharmaceutically acceptable carrier, wherein the medicament acts by inhibiting AKT-IκB-NF κB signaling pathway, and wherein the nano-curcumin is obtained by pulverizing curcumin into uniform nano-scale particles.

Description

Network pharmacological target screening method for application of nano curcumin to plateau skin burn and wound composite injury and application of nano curcumin in preparation of medicament for treating plateau burn and wound composite injury Technical Field The invention relates to the technical field of medicines, in particular to a network pharmacological target screening method for applying nano curcumin to plateau skin burn injury complex injury and application of the nano curcumin in preparation of medicines for treating the plateau skin burn injury complex injury. Background Treatment of burn wounds is a significant clinical challenge in high altitude, low temperature, low oxygen, altitude, plateau environments. In special environments, skin wounds are easy to develop into persistent and unhealed wounds, so that acceleration of wound healing is particularly important. Traditional curcumin has remarkable antioxidant and anti-inflammatory activities, however, clinical application is often limited by low bioavailability, low solubility and the like. Nano curcumin is a nano preparation of curcumin, and the curcumin has various biological activities such as anti-inflammatory, antioxidant, antibacterial and the like. However, the application of nano curcumin in treating high-altitude skin burn and wound composite wounds has not been fully studied. Network pharmacology is an emerging research method, and can systematically analyze the interaction relationship between drugs and diseases and screen out potential therapeutic targets. However, no related report exists on the application of the network pharmacology method to the screening of the composite wound targets of the plateau skin burn treatment by the nano curcumin. Therefore, the development of a network pharmacological target screening method of the nano curcumin applied to the plateau skin burn and wound composite injury and the exploration of the application of the nano curcumin in preparing the medicine for treating the plateau skin burn and wound composite injury have important clinical significance and social value, and are expected to provide a new thought and method for the medicine for treating the plateau skin burn and wound composite injury. Disclosure of Invention In view of the defects existing in the prior art, the main purpose of the invention is to provide a network pharmacology target screening method for applying nano curcumin to plateau skin burn injury complex injury and application of the nano curcumin in preparation of medicaments for treating the plateau burn injury complex injury, three key signal channel proteins of AKT, iκB and NF κB are successfully screened out as core action targets by constructing a protein-protein interaction network, and the nano curcumin provides a novel medical strategy and a solid experimental basis for promoting healing of the burn injury complex injury in a special plateau environment by accurately regulating and controlling AKT/iκB/NF κB signal channels. In order to achieve the above purpose, the present invention adopts the following technical scheme: A network pharmacology target screening method for applying nano curcumin to plateau skin burn and wound compound injury comprises the following steps: s1, obtaining a curcumin-related protein target; s2, obtaining a protein target spot related to the plateau burn wound; s3, constructing a protein interaction network and selecting a key target point; S4, cross target enrichment analysis, namely guiding the common target of the nanocrystalline curcumin aiming at the burn wound into a metscape platform to obtain GO and KEGG enrichment data; S5, molecular docking, namely, receptor protein is NF kappa B (PDB ID:1 SVC), a semi-flexible docking mode is set, a docking algorithm is a Ramahk genetic algorithm, and Auto Dock Vina.2.5 software is operated to carry out molecular docking, so that docking combination free energy and a docking result file are obtained; S6, molecular dynamics simulation, namely performing molecular dynamics simulation on the complex of the NF- κB protein and the curcumin by using software. The application of the nano curcumin obtained by the network pharmacological target screening method of the nano curcumin applied to the plateau skin burn and wound composite injury in preparing the medicine for treating the plateau burn and wound composite injury. As a preferable scheme, the nano curcumin is used for preparing a medicament for treating the composite wound of the plateau burn, and the dosage of the nano curcumin in the medicament is 25mg/ml-50mg/ml. As a preferable scheme, the nano curcumin in the medicament plays a role in promoting recovery in early stage of wound healing by promoting wound healing of burn and wound composite injury, and the dosage of the nano curcumin in the medicament is 45mg/ml. As a preferable scheme, the nano curcumin is used for preparing a medicament for regulating and controlling an AKT/iκB/NF κB signal