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CN-122003175-A - Ascaroside and phytonutrient combination and methods of use

CN122003175ACN 122003175 ACN122003175 ACN 122003175ACN-122003175-A

Abstract

Provided herein are combinations of agents for enhancing plant growth and methods of use thereof. The combination comprises at least one ascaroside (or derivative or analogue of ascaroside) and at least one plant nutrient. The one or more ascarosides and one or more phytonutrients may be provided in the same composition, or may be applied together (e.g., substantially simultaneously) or sequentially.

Inventors

  • Muli Manohar
  • EDISON HIDALGO
  • Gabriel Vilmos

Assignees

  • 归因生物科技有限公司

Dates

Publication Date
20260508
Application Date
20240724
Priority Date
20230725

Claims (20)

  1. 1. A method for providing nutrients and protection against pathogens to a plant, the method comprising contacting the plant, plant part, or soil surrounding the plant with an effective amount of a combination of agents comprising one or more ascarosides and one or more plant nutrients.
  2. 2. A method for providing nutrients and protection against pathogens to a plant, the method comprising applying ascaroside to a plant, plant part, or soil surrounding the plant or plant part, wherein the plant, plant part, or soil surrounding the plant or plant part is receiving or has received nutrients (e.g., via application to the plant, plant part, or soil surrounding the plant or plant part).
  3. 3. A method for providing nutrients and protection against pathogens to a plant, the method comprising applying nutrients to a plant, plant part, or soil surrounding the plant or plant part, wherein the plant, plant part, or soil surrounding the plant or plant part is receiving or has received ascarosides (e.g., via application to the plant, plant part, or soil surrounding the plant or plant part).
  4. 4. The method of claim 1, wherein the one or more plant nutrients comprise one or more macronutrients selected from the group consisting of nitrogen-containing nutrients, phosphorus-containing nutrients, potassium-containing nutrients, calcium-containing nutrients, magnesium-containing nutrients, sulfur-containing nutrients, and combinations thereof.
  5. 5. The method of claim 1, wherein the one or more plant nutrients comprise one or more micronutrients selected from the group consisting of boron-containing nutrients, chlorine-containing nutrients, copper-containing nutrients, iron-containing nutrients, manganese-containing nutrients, molybdenum-containing nutrients, zinc-containing nutrients, and combinations thereof.
  6. 6. The method of claim 1, wherein the one or more phytonutrients comprise one or more macronutrients and one or more micronutrients.
  7. 7. The method of any one of claims 1-6, wherein the one or more ascarosides comprises a ascaroside having structure (I): Wherein: Z is an optionally substituted C 3-40 aliphatic group, and Each of R a and R b is independently-H or an optionally substituted moiety selected from the group consisting of C 1-20 aliphatic, C 1-20 acyl, C 1-20 heteroaliphatic, aryl, heteroaryl, hydroxy protecting group, phosphorus-attached functional group, sulfur-attached functional group, silicon-attached functional group, C 2-20 carbonates (e.g., part-C (O) OR c ）、C 2-20 carbamates (e.g., part-C (O) N (R c ) 2 ）、C 2-20 thioesters (e.g., part-C (S) R c ）、C 2-20 thiocarbonates (e.g., part-C (S) OR c ）、C 2-20 dithiocarbonates (e.g., part-C (S) SR c ）、C 1-20 thiocarbamates (e.g., part-C (S) N (R c ) 2 ), A sugar moiety, a peptide, a polymer chain, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety. Wherein R c at each occurrence is independently selected from-H, an optionally substituted C 1-12 aliphatic, an optionally substituted C 1-12 heteroaliphatic, an optionally substituted aryl, an optionally substituted heteroaryl, a polymer chain, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety, and wherein R a and R b may together form an optionally substituted ring optionally containing one or more heteroatoms and optionally containing one or more sites of unsaturation.
  8. 8. The method of claim 7, wherein Z is selected from the group consisting of: (i) -CH (CH 3 )–R 1 , wherein R 1 is an optionally substituted C 1-40 aliphatic; (ii) -CH (CH 3 )–(CH 2 ) n –CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (iii) -CH (CH 3 )–(CH 2 ) n –CH=CH-CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (iv) -CH (CH 3 )–(CH 2 ) n –CH(OH)–CH 2 -CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (v) -CH (CH 3 )–(CH 2 ) n –C(O)–CH 2 -CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (vi) - (CH 2 ) n –CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (vii) - (CH 2 ) n –CH=CH-CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide; (viii) - (CH 2 ) n –CH(OH)–CH 2 -CO 2 R 2 ) wherein n is an integer from 1 to 40 and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide, and (Ix) - (CH 2 ) n –C(O)–CH 2 -CO 2 R 2 , wherein n is an integer from 1 to 40, and R 2 is-H, a metal cation, an optionally substituted C 1-20 aliphatic group, an optionally substituted aromatic group, a glycoside, an amino acid, a peptide or a nucleotide.
  9. 9. The method of claim 7, wherein Z is selected from the group consisting of: (x) -CH (CH 3 )–(CH 2 ) n –CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xi) -CH (CH 3 )–(CH 2 ) n –CH=CH-CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xii) -CH (CH 3 )–(CH 2 ) n –CH(OH)–CH 2 -CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xiii) -CH (CH 3 )–(CH 2 ) n –C(O)–CH 2 -CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xiv) - (CH 2 ) n –CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl group, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xv) - (CH 2 ) n –CH=CH-CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl group, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xvi) - (CH 2 ) n –CH(OH)–CH 2 -CON(R 3 ) 2 , wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl group, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety; (xvii) - (CH 2 ) n –C(O)–CH 2 -CON(R 3 ) 2 ) wherein n is an integer from 1 to 40, and each R 3 is independently-H, an optionally substituted C 1-20 aliphatic, an optionally substituted C 1-20 heteroaliphatic, an optionally substituted aromatic, an optionally substituted heteroaryl, a polymer chain, an amino acid, a peptide, a nucleotide, or a linkage to another ascaroside molecule via a bond or a carbon-containing linker moiety, and (Xix) An optionally unsaturated optionally substituted C 2-40 side chain terminating at the chain end comprising a nitrogen-, oxygen-or sulfur-containing functional group.
  10. 10. The method of any one of claims 1 to 9, wherein the one or more ascarosides comprise ascarosides selected from the group consisting of ascr#9, ascr#10, ascr#16, ascr#18, ascr#20, ascr#22, and ascr#24.
  11. 11. The method of any one of claims 1 to 9, wherein the one or more ascarosides comprises ascr#18.
  12. 12. The method of any one of claims 1 to 11, wherein the one or more ascarosides and the one or more phytonutrients are applied simultaneously.
  13. 13. The method of claim 12, wherein the one or more ascarosides and the one or more phytonutrients are contained in the same composition.
  14. 14. The method of any one of claims 1 to 13, wherein the one or more ascarosides and the one or more phytonutrients are applied sequentially.
  15. 15. The method of any one of claims 1 to 14, wherein the combination is used as a seed coating.
  16. 16. A composition comprising one or more ascarosides and one or more phytonutrients.
  17. 17. The composition of claim 16, wherein the one or more ascarosides and the one or more phytonutrients are present in an effective amount.
  18. 18. The composition of claim 16 or 17, wherein the one or more plant nutrients comprise one or more macronutrients selected from the group consisting of nitrogen-containing nutrients, phosphorus-containing nutrients, potassium-containing nutrients, calcium-containing nutrients, magnesium-containing nutrients, sulfur-containing nutrients, and combinations thereof.
  19. 19. The composition of claim 16 or 17, wherein the one or more plant nutrients comprise one or more micronutrients selected from the group consisting of boron-containing nutrients, chlorine-containing nutrients, copper-containing nutrients, iodine-containing iron-containing nutrients, manganese-containing nutrients, molybdenum-containing nutrients, zinc-containing nutrients, and combinations thereof.
  20. 20. The composition of claim 16 or 17, wherein the one or more plant nutrients comprise one or more macronutrients and one or more micronutrients.

Description

Ascaroside and phytonutrient combination and methods of use Cross Reference to Related Applications The present application claims priority from U.S. provisional application No. 63/515,554 filed on 7.25.2023, the entire contents of which are hereby incorporated by reference. Technical Field The present invention relates to compositions and methods relating to the use of ascarosides and one or more phytonutrients. Background Plant nutrients are very important for the growth and development of plants and for the production and quality of flowers and fruits. Plant nutrients are also required for plants to produce chlorophyll and to perform photosynthesis. Many essential and beneficial plant nutrients have been identified, each having an important role in promoting the growth and development of plants, flowers and fruits. One or more (and often more than one) plant nutrients are typically applied to any given crop-producing plant ("crop") during the growth of the plant. In order to effectively address plant nutrient deficiencies and prevent pathogen damage in plant life, it is often necessary to apply these components separately. This is detrimental because it requires additional passes over the crop, which increases costs, resource usage (e.g., fuel, water, labor, etc.), and potential damage to the crop and/or soil. Disclosure of Invention Combinations of agents for promoting plant growth and methods of use thereof are provided. The combination comprises at least one ascaroside (or derivative or analogue of ascaroside) and at least one plant nutrient. Ascarosides may be combined with at least one plant nutrient to provide the plant with its components necessary for growth while also providing durable protection against pathogens (e.g., fungi, mold, bacteria, viruses, and/or nematodes) during plant life. The one or more ascarosides and the one or more nutrients may be provided in the same composition, or may be applied together (i.e., substantially simultaneously) or sequentially. The agent is applied in an effective amount (i.e., an amount sufficient to provide one or more nutrients to the plant to promote growth and development and also provide durable protection against the pathogen). The present invention includes, but is not limited to, the following examples. Example 1A method for providing nutrients and protection against pathogens to a plant comprising contacting the plant, plant part, or soil surrounding the plant with an effective amount of a combination of agents comprising one or more ascarosides and one or more phytonutrients. Embodiment 2 the method of embodiment 1 wherein the one or more plant nutrients comprise one or more macronutrients selected from the group consisting of nitrogen-containing nutrients, phosphorus-containing nutrients, potassium-containing nutrients, calcium-containing nutrients, magnesium-containing nutrients, sulfur-containing nutrients, and combinations thereof. Embodiment 3 the method of embodiment 1 or 2 wherein the macronutrient is selected from the group consisting of a nitrogen-containing nutrient, a phosphorus-containing nutrient, a potassium-containing nutrient, and combinations thereof. Embodiment 4 the method of any one of embodiments 1 to 3, wherein the one or more phytonutrients are in the form of a mixture of nitrogen-containing nutrients, phosphorus-containing nutrients, and potassium-containing nutrients. Embodiment 5 the method of embodiment 2 wherein the macronutrient is selected from the group consisting of a calcium-containing nutrient, a magnesium-containing nutrient, a sulfur-containing nutrient, and combinations thereof. Embodiment 6 the method of any one of embodiments 1 to 5 wherein the one or more phytonutrients are in the form of manure/guano, fish milk, bone meal, blood meal, biofertilizer or cover crop. Embodiment 7 the method of any one of embodiments 1-6 wherein the one or more plant nutrients comprise one or more micronutrients selected from the group consisting of boron-containing nutrients, chlorine-containing nutrients, copper-containing nutrients, iron-containing nutrients, manganese-containing nutrients, molybdenum-containing nutrients, zinc-containing nutrients, and combinations thereof. Embodiment 8 the method of any one of embodiments 1 to 7, wherein the one or more phytonutrients comprise one or more macronutrients and one or more micronutrients. Embodiment 9 the method of any one of embodiments 1-8, wherein the one or more ascarosides comprises a ascaroside having structure (I): Wherein Z is an optionally substituted C 3-40 aliphatic group and each of R a and R b is independently-H or an optionally substituted moiety selected from the group consisting of C 1-20 aliphatic, C 1-20 acyl, C 1-20 heteroaliphatic, aryl, heteroaryl, hydroxy protecting group, phosphorus-attached functional group, sulfur-attached functional group, silicon-attached functional group, C 2-20 carbonates (e.g., part-C (O) OR c）、C2-20 carbamates (e.g., part-C (O