CN-122003181-A - Feed additive for improving performance of farmed animals

Abstract

The present invention is in the field of feed additives for animals (e.g., farmed animals), particularly feed additives that are added to animal feed to obtain improved performance of such animals (e.g., increase daily gain and/or increase feed efficiency (i.e., decrease feed conversion rate)). In addition, the invention also relates to a culture medium and a cell culture method.

Inventors

• David Manuel Bravo
• Karin Beagle Bitter

Assignees

• 纽崔克IP资产有限公司

Dates

Publication Date

20260508

Application Date

20240814

Priority Date

20230814

Claims (15)

1. 1. An animal feed comprising blackberry leaves.
2. 2. The animal feed of claim 1, wherein the blackberry leaves are dried and optionally ground.
3. 3. The animal feed of claim 1 or 2, wherein the blackberry leaves are included in an amount of about 1 mg/kg feed to about 10 g/kg feed.
4. 4. Use of blackberry leaves as feed additive.
5. 5. The use according to claim 4, wherein the feed additive is provided with written instructions to include the feed additive in animal feed in an amount of about 1 mg/kg feed to about 10000 mg/kg feed, preferably 5 mg/kg feed to about 5000 mg/kg feed.
6. 6. The use according to claim 4 or 5, wherein the feed additive is included in a premix.
7. 7. Use of blackberry leaves or an extract thereof or a composition comprising blackberry leaves or an extract thereof or an animal feed according to any one of claims 1-3 for increasing the performance of an animal.
8. 8. Use of blackberry leaves or extracts thereof or a composition comprising blackberry leaves or extracts thereof or an animal feed according to any one of claims 1-3 for feeding an animal, increasing feed intake, increasing daily gain, increasing feed efficiency (i.e. reducing feed conversion rate), increasing relative growth rate and/or increasing specific growth rate.
9. 9. Use according to claim 7 or 8, wherein the extract is selected from the group consisting of aqueous extract, ethanol extract, methanol extract, isopropanol extract, ethyl acetate extract, acetone extract, hexane extract or mixtures of these solvents or supercritical CO2 extract, or mixtures of any of these.
10. 10. Use according to any one of claims 4-9, wherein the feed additive is intended to be included in a feed for farmed animals.
11. 11. The use according to claim 10, wherein the farmed animals are selected from the group consisting of poultry, pigs, ruminants such as beef cattle and dairy cows, fish such as salmon, trout, sea bream, sea bass, tilapia, tuna, etc., and crustaceans such as shrimp.
12. 12. Use of an extract of blackberry leaves for culturing muscle cells and/or adipocytes, for increasing glucose uptake in cells (e.g., muscle cells and/or adipocytes), and/or for increasing cell proliferation (e.g., muscle cell proliferation and/or adipocyte proliferation).
13. 13. Use according to claim 12, wherein the cultured cells are for the purpose of preparing cultured meat or cultured fish.
14. 14. A cell culture medium comprising an extract of blackberry leaves.
15. 15. A method of culturing muscle cells and/or adipocytes, comprising the steps of providing a cell culture medium according to claim 14, inoculating the medium with cells to be cultured, and proliferating the cells.

Description

Feed additive for improving performance of farmed animals Technical Field The invention is in the field of feed additives for animal feeds, in particular for improving the performance of farmed animals. Furthermore, the invention is also in the field of cell culture, for example of muscle cells, for example for the preparation of cultured meat. Background Antimicrobial agents have been used for decades as antibiotic growth promoters in livestock feed. However, resistance to such antimicrobial agents is currently considered a serious threat to human health. Since month 1 in 2006, the european union has completely banned the use of antimicrobial agents as growth promoters in livestock feed. Meanwhile, the demand for proteins from animal sources has increased, driven by the growth of the population. In addition, food production needs to be more sustainable, requiring less (investment) to produce more (product). In summary, animal production requires greater and greater efficiency. These developments have triggered a hot tide of developing feed additives that improve the performance of the farmed animals without inducing resistance to bacteria. Feed additives derived from plants, also known as vegetable products, are substances of vegetable origin which are added to the animal diet for the purpose of improving animal performance. Essential oils, herbs and spices are all used as sources for bioactive ingredients (e.g. phenols, polyphenols, flavonoids, terpenes and others). It is an object of the present invention to provide compositions that enhance glucose uptake in cells, increase cell proliferation and/or enhance performance of farmed animals. It is another object of the present invention to provide a method of increasing weight gain, increasing daily average weight gain, increasing feed intake, and/or decreasing Feed Conversion Ratio (FCR) (i.e., increasing feed efficiency) in a farm animal. Further, it is an object of the present invention to provide a composition that increases glucose uptake in muscle cells and/or increases cell proliferation in muscle cells. Such a composition may be part of a cell culture medium, particularly for culturing muscle cells to produce cultured meat. Disclosure of Invention The present disclosure provides animal feeds comprising blackberry leaves or extracts thereof. The blackberry leaves can be dried, and optionally crushed. Blackberry leaves, or equivalent amounts of extract, are included in amounts of about 1 mg/kg feed to about 10 g/kg feed. The extract may be selected from the group consisting of an aqueous extract, an ethanol extract, a methanol extract, an isopropanol extract, an ethyl acetate extract, an acetone extract, a hexane extract or a supercritical CO2 extract, or a mixture of any of these. The present disclosure also provides for the use of blackberry leaves or extracts thereof as a feed additive. The feed additive may be provided with written instructions to include the feed additive in an amount of from about 1 mg/kg feed to about 10000 mg/kg feed, preferably from 5 mg/kg feed to about 5000 mg/kg feed, blackberry leaves or an equivalent amount of extract into an animal feed. The feed additive may be included in a premix. The present disclosure further provides the use of blackberry leaves or extracts thereof or compositions comprising blackberry leaves or extracts thereof or animal feeds as taught herein for increasing the performance of an animal. The present disclosure also provides the use of blackberry leaves or extracts thereof, or compositions comprising blackberry leaves or extracts thereof, or animal feed as taught herein, for increasing feed intake, increasing daily gain, increasing feed efficiency (i.e., reducing feed conversion rate), increasing relative growth rate, and/or increasing specific growth rate. The extract may be selected from the group consisting of aqueous extract, ethanol extract, methanol extract, isopropanol extract, ethyl acetate extract, acetone extract, hexane extract or mixtures of these solvents or supercritical CO2 extract, or mixtures of any of these. The feed additive may be intended to be included in a feed for farmed animals. The farmed animals may be selected from the group consisting of poultry, pigs, ruminants such as beef cattle and dairy cows, fish such as salmon, trout, sea bream, sea bass, tilapia, tuna, etc., and crustaceans such as shrimp. The present disclosure also provides for the use of an extract of blackberry leaves for culturing cells (e.g., muscle cells and/or adipocytes), for increasing glucose uptake in cells (e.g., muscle cells and/or adipocytes), and/or for increasing cell proliferation (e.g., muscle cell proliferation and/or adipocyte proliferation). The cells may be cultured for the purpose of preparing cultured meat, including cultured fish. The present disclosure further provides a cell culture medium comprising an extract of blackberry leaves. Furthermore, the present disclosure relates to a me