CN-122003242-A - Composition for protecting tight junctions

Abstract

The invention aims to provide a novel application of EPS of lactic acid bacteria. Further, the present invention aims to provide a composition for protecting tight junctions or a composition for promoting gene expression of tight junction constituent molecules. According to the present invention, there is provided a composition for protecting tight junctions, or a composition for promoting the expression of a tight junction protein-1 (ZO-1) gene, the composition comprising EPS of lactic acid bacteria.

Inventors

• HIROKI ISHIKAWA
• SASHIHARA TOSHIHIRO
• Yokoo takehiro

Assignees

• 株式会社明治

Dates

Publication Date

20260508

Application Date

20240809

Priority Date

20230810

Claims (17)

1. 1. A composition for protecting tight junctions, which comprises extracellular polysaccharide of lactic acid bacteria, the tight junctions being tight junctions of lung cells.
2. 2. The composition of claim 1, wherein protecting the tight junction is promoting recovery of the damaged tight junction or inhibiting damage to the tight junction.
3. 3. The composition of claim 2, wherein the injury is caused by a viral infection.
4. 4. The composition of claim 2, wherein the injury is a reduction in expression of zonulin-1, ZO-1, gene.
5. 5. A composition according to claim 3, wherein the virus is an influenza virus.
6. 6. The composition of claim 1, wherein the lactic acid bacteria are exopolysaccharide-producing bacteria.
7. 7. The composition of claim 1, wherein the lactic acid bacteria are selected from the group consisting of bacteria classified as lactobacillus delbrueckii subsp bulgaricus, bacteria classified as lactococcus lactis subsp lactis, and bacteria classified as lactococcus lactis subsp.
8. 8. The composition according to claim 1, wherein the lactic acid bacteria is lactobacillus delbrueckii subspecies bulgaricus OLL1073R-1 (FERM BP-10741).
9. 9. The composition of claim 1 for ingestion by any one selected from the group consisting of a person over 65 years old, a child, an infant, a neonate, a person suffering from a chronic respiratory disease, and a smoker.
10. 10. The composition of claim 2, wherein promoting recovery of tight junctions or inhibiting damage to tight junctions is achieved by promoting ZO-1 gene expression.
11. 11. A composition for promoting expression of a ZO-1 gene comprising an extracellular polysaccharide of a lactic acid bacterium.
12. 12. The composition of claim 11 for promoting expression of ZO-1 gene in damaged tight junctions.
13. 13. The composition of claim 11, wherein the lactic acid bacteria are exopolysaccharide-producing bacteria.
14. 14. The composition of claim 11, wherein the lactic acid bacteria are lactic acid bacteria classified as lactobacillus delbrueckii subsp bulgaricus.
15. 15. The composition of claim 11, wherein the lactic acid bacteria are selected from the group consisting of bacteria classified as lactococcus lactis subspecies lactis and bacteria classified as lactococcus lactis subspecies cremoris.
16. 16. A process for producing a fermented milk for protecting tight junctions, which comprises adding and fermenting lactic acid bacteria classified as Lactobacillus delbrueckii subspecies bulgaricus and lactic acid bacteria classified as Streptococcus thermophilus to a prepared milk liquid containing raw milk.
17. 17. An inhalant comprising an extracellular polysaccharide of a lactic acid bacterium.

Description

Composition for protecting tight junctions Technical Field The present invention relates to a composition for protecting tight junctions. Background Tight junctions are intercellular adhesion devices that exist in epithelial cells, vascular endothelial cells. The tight junctions are mainly composed of a closed protein (Occludin) and a dense protein (Claudin) as transmembrane proteins, a zona occludens (ZO: zonula occludens) as cytoskeletal proteins, and the like. The tight junctions form a barrier by mechanically connecting cells to each other, and play an important role in preventing invasion of foreign substances such as pathogenic bacteria and toxins. Regarding reinforcement of barrier function, patent document 1 describes a composition comprising Lactobacillus species which proliferate as a biofilm and/or an extract comprising the biofilm, and in the case where the composition is applied to a surface, the biofilm and/or the extract thereof reinforce barrier function so as to make the surface less susceptible to microbial, viral and/or chemical contamination. Patent document 3 describes a skin-quality improver containing lactobacillus rhamnosus (Lactobacillus rhamnosus) cells or an extract of the cells as an active ingredient, and shows that the amount of trans-epidermal water evaporation from the skin is reduced by applying a sterilized powder cream of lactobacillus rhamnosus KO3 strain. Non-patent document 1 describes that lactic acid bacteria act on intestinal immunity and on intestinal epithelial cells to increase expression of tight junctions and mucin production, thereby improving intestinal barrier function. Non-patent document 2 suggests that lactobacillus rhamnosus OLL2838 improves intestinal barrier dysfunction. Non-patent document 3 suggests that Lactobacillus fermentum (Lactobacillus fermentum), lactobacillus helveticus (Lactobacillus helveticus), lactobacillus salivarius (Lactobacillus salivarius) or Lactobacillus plantarum (Lactobacillus plantarum) improve intestinal epithelial barrier dysfunction. Patent document 4 describes a lactobacillus plantarum LOC1 strain, and discloses that the strain increases expression of a specific tight junction related gene in intestinal cells, and the like. On the other hand, it was confirmed that Extracellular Polysaccharide (EPS) produced by lactic acid bacteria brings about various health effects. Patent document 2 describes a composition for preventing or reducing the risk of onset of a secondary infection after a viral infection, which comprises an extracellular polysaccharide of lactic acid bacteria. In this document, it is described that prevention of secondary infection after virus infection or reduction of risk of occurrence is achieved by inhibiting expression of carcinoembryonic antigen-related cell adhesion molecule 1 (Carcinoembryonic antigen-RELATED CELL adhesion molecule, ceacam-1), and that EPS has no effect on mRNA expression of a tightly-linked molecule under experimental conditions. Prior art literature Patent literature Patent document 1 Japanese patent application laid-open No. 2023-503350 Patent document 2 International publication WO2023/120547 Patent document 3 Japanese patent application laid-open No. 2022-182870 Patent document 4 Japanese patent laid-open No. 2022-73988 Non-patent literature Non-patent document 1: sha Zhi in the field, a food-derived ingredient, i.e., a component よ, which acts as an immune therapy, a component which acts as a base of a food, a component which acts as a base of an immune therapy (i.e., a component which acts as an immune therapy based on food ingredients: food-derived ingredient, i.e., よ -protective immune motor, i.e., kidney-energy-producing food. The physical therapy of the disease, the prevention of the disease (immunomodulatory effects based on food ingredients Non-patent literature 2：E Miyauchi, H Morita, S Tanabe, Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of zonula occludens-1 and myosin light-chain kinase in vivo. J Dairy Sci. 2009 Jun;92(6)：2400-8. doi： 10.3168/jds.2008-1698. Non-patent literature 3：Ruchi Jariwala, Hemanti Mandal and Tamishraha Bagchi, Indigenous lactobacilli strains of food and human sources reverse enteropathogenic E. coli O26：H11-induced damage in intestinal epithelial cell lines： effect on redistribution of tight junction proteins. Microbiology 2017;163：1263-1272 Disclosure of Invention Problems to be solved by the invention The invention aims to provide a novel application of EPS of lactic acid bacteria. Further, the present invention aims to provide a composition for protecting tight junctions or a composition for promoting gene expression of tight junction constituent molecules. Solution for solving the problem Namely, the present invention relates to the following. [1] A composition for protecting tight junctions comprising an extracellular polysaccharide of a lactic acid bacterium. [2] The composition according