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CN-122003599-A - Method and device for determining biomolecules

CN122003599ACN 122003599 ACN122003599 ACN 122003599ACN-122003599-A

Abstract

According to one embodiment of the present invention, a method of assaying a biomolecule may be provided. The method comprises providing a recognition means comprising a conductive polymer and a molecular recognition pH indicator that functions as a dopant for the conductive polymer and recognizes a target biomolecule, introducing a sample containing the target biomolecule into the recognition means, measuring an electrical characteristic of the conductive polymer based on a reaction between the target biomolecule and the molecular recognition pH indicator that occurs when the target biomolecule is introduced, and determining the amount of the target biomolecule in the sample based on the electrical characteristic obtained by the measurement.

Inventors

  • SAKATA TOSHIYA
  • Alex Zhiwei once, ever, previously
  • ITO NARUSHI

Assignees

  • 国立大学法人东京大学
  • 株式会社普欧威盖特

Dates

Publication Date
20260508
Application Date
20240823
Priority Date
20230829

Claims (17)

  1. 1. A method of determining a biomolecule, comprising the steps of: Providing a recognition unit comprising a conductive polymer and a molecular recognition pH indicator that functions as a dopant for the conductive polymer and recognizes a target biomolecule; Introducing a sample containing the target biomolecule into the recognition unit; Determining an electrical property of the conductive polymer based on a reaction of the target biomolecule with the molecular recognition pH indicator that occurs upon introduction of the target biomolecule, and Based on the electrical characteristics obtained by the measurement, the amount of the target biomolecule in the sample is determined.
  2. 2. The method of claim 1, wherein the molecular recognition pH indicator is disposed in a network of the conductive polymer.
  3. 3. The method of claim 1, wherein the identification unit is further provided with a second polymer disposed in the network of conductive polymers.
  4. 4. A method according to claim 3, wherein the second polymer has the ability to function as a dopant for the conductive polymer.
  5. 5. The method of claim 1, wherein, The target biological molecule is albumin and the target biological molecule is the albumin, The molecular recognition pH indicator is BCP or BCG, The conductive polymer comprises PEDOT.
  6. 6. The method of claim 5, wherein the identification unit is further provided with a PSS disposed in a network of the conductive polymer.
  7. 7. A method for obtaining GA values, comprising the steps of: Providing a sample containing albumin; determining the amount of albumin in the sample using the method of claim 5 or 6; determining the amount of glycated albumin in the sample, and The GA value of the sample is obtained from the amount of albumin and the amount of glycated albumin.
  8. 8. An apparatus for measuring a biological molecule, comprising: A recognition unit comprising a conductive polymer and a molecular recognition pH indicator that functions as a dopant for the conductive polymer and recognizes a target biomolecule, and A transducer that converts a reaction of the molecular recognition pH indicator with the target biomolecule that occurs upon introduction of the target biomolecule into an electrical signal.
  9. 9. The device of claim 8, further comprising a circuit coupled to the transducer and configured to process the electrical signal based on the reaction of the molecular recognition pH indicator with the target biomolecule.
  10. 10. The apparatus of claim 8, wherein, The transducer is provided with an electrode which, The conductive polymer and the molecular recognition pH indicator are disposed on the surface of the electrode, The device further includes a circuit connected to the electrode, wherein the circuit is configured to evaluate a redox reaction of the conductive polymer related to a reaction of the molecular recognition pH indicator with the target biomolecule, which occurs when the target biomolecule is introduced.
  11. 11. The apparatus of claim 8, wherein, The device is provided with a drain electrode and a source electrode, The conductive polymer is arranged in such a manner as to connect the drain electrode and the source electrode, The device includes a circuit connected to the drain electrode and the source electrode, and the circuit is configured to measure transistor characteristics between the drain electrode and the source electrode, the transistor characteristics being related to a reaction between the molecular recognition pH indicator and the target biomolecule, the reaction occurring when the target biomolecule is introduced.
  12. 12. An apparatus for measuring albumin, comprising: a recognition unit comprising PEDOT, and BCP or BCG, and A transducer for detecting a reaction between the BCP or BCG and albumin, which occurs when albumin is introduced.
  13. 13. A GA value measurement device is provided with: An albumin measuring unit comprising a recognition unit comprising PEDOT and BCP or BCG, a transducer for detecting a reaction between the BCP or BCG and albumin, which occurs when albumin is introduced, and And a unit for assaying the glycated albumin.
  14. 14. A fluidic device for GA value measurement, comprising: an albumin measurement chamber having a recognition unit including PEDOT and BCP or BCG, a transducer for detecting a reaction between the BCP or BCG and albumin, which occurs when albumin is introduced, and The chamber for measuring glycated albumin has a unit for measuring glycated albumin disposed therein.
  15. 15. The fluidic device of claim 14, wherein, The albumin-measuring chamber is in fluid connection with the glycated albumin-measuring chamber, The glycated albumin assay chamber is disposed downstream of the albumin assay chamber.
  16. 16. The fluidic device according to claim 14, wherein the sample introduced into the fluidic device is split and introduced into the albumin measurement chamber and the glycated albumin measurement chamber, respectively.
  17. 17. The fluidic device of claim 14, wherein, The fluid device further comprises a measuring fluid chamber, The measurement fluid chamber accommodates both an albumin measurement unit and a glycated albumin measurement unit therein.

Description

Method and device for determining biomolecules Technical Field The present invention relates to a method and apparatus for determining biomolecules. Background In the field of biosensing, there is a need for a technique capable of more accurately and more easily measuring biomolecules present in a solution such as a body fluid. For example, GA values are attracting attention as important indicators for blood glucose management. There is a need for a technique for simply and accurately determining the GA value. The GA value is the ratio of the amount of glycated albumin to the total amount of albumin. Biosensing technology for glycated albumin has been developed. On the other hand, measurement of the total amount of albumin is usually performed by BCP method, BCG method, HPLC method, immunoturbidimetry, ELISA (enzyme-linked immunosorbent assay), high Performance Liquid Chromatography (HPLC) precipitation method, or the like, but these methods are liquid phase method or in-solution measurement method. Therefore, the development of biosensors for measuring albumin has been delayed. Disclosure of Invention Here, the necessity of a technique for measuring various substances (e.g., proteins, etc.) not limited to the total amount of albumin, for example, but not limited to, in a body fluid, simply and easily using a biosensor is recognized. Some embodiments of the invention may provide methods of assaying biomolecules. In some embodiments, the method includes the step of providing a recognition unit comprising a conductive polymer and a molecular recognition pH indicator that functions as a dopant for the conductive polymer and recognizes a target biomolecule. In some embodiments, the method comprises the step of introducing a sample comprising the target biomolecule into the recognition unit. In some embodiments, the method includes the step of determining an electrical characteristic of the conductive polymer based on a reaction of the target biomolecule with the molecular recognition pH indicator that occurs upon introduction of the target biomolecule. In some embodiments, the method includes the step of determining the amount of the target biomolecule in the sample based on the electrical characteristics obtained by the measurement. According to the above embodiment, for example, the amount of biomolecules in body fluid can be simply and accurately measured using a biosensor. Further aspects and advantages of the present invention will become readily apparent to those skilled in the art from the following detailed description, which shows and illustrates only exemplary embodiments of the invention. As will be realized, the invention is capable of other and different embodiments and its several details are capable of modifications in various obvious aspects all without departing from the invention. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive. Drawings FIG. 1 is a cyclic voltammogram of a PEDOT: BCP system relative to various concentrations of HSA solution for one embodiment. Fig. 2 is a graph showing the relationship between the concentration of HSA solution and the height of the oxidation peak of the cyclic voltammogram shown in fig. 1. Fig. 3 is a schematic diagram showing the constitution of the apparatus of one embodiment and the functions thereof. Fig. 4 is a schematic diagram showing the constitution of an apparatus of one embodiment and the functions thereof. Fig. 5 is a schematic diagram showing the constitution of the apparatus of one embodiment and the functions thereof. Fig. 6 is a schematic diagram showing the configuration of an apparatus for GA value measurement according to an embodiment. Fig. 7 is a schematic diagram showing the configuration of an apparatus for GA value measurement according to an embodiment. Fig. 8 is a schematic diagram showing the configuration of an apparatus for GA value measurement according to an embodiment. FIG. 9A1 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p1 FIG. 9A2 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p2 FIG. 9A3 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p3 FIG. 9A4 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p4 FIG. 9A5 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p5 FIG. 9A6 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p6 FIG. 9A7 is a graph showing the evaluation of PEDOT: BCP electrochemical electrode in human serum albumin measurement by dye binding method, p7 FIG. 9A8