CN-122003603-A - Enzyme solution and method for evaluating biodegradability of biodegradable resin using the same

Abstract

The present disclosure relates to an enzyme solution having biodegradability of a biodegradable resin and useful for evaluating biodegradability of a biodegradable resin, and a biodegradability evaluation method for a biodegradable resin using the same.

Inventors

• Ding Xizheng
• ZHANG SHIYONG
• Ding Bingzhun
• JIN DUHENG
• LI TINGBIN
• Pu Guangpei
• Pu Jixie
• SHEN MINHE
• LIN YONGJI
• JIN XIANZHEN
• BAI SONGYI

Assignees

• 株式会社LG化学

Dates

Publication Date

20260508

Application Date

20250903

Priority Date

20240903

Claims (16)

1. 1. An enzyme solution comprising: A hydrolase comprising a lipase and a cutinase, and Phosphate buffered saline.
2. 2. The enzyme solution according to claim 1, Wherein the enzyme unit ratio of lipase to cutinase is 0.5:1 to 4:1.
3. 3. The enzyme solution according to claim 1 or 2, Wherein the total amount of hydrolase in the enzyme solution of 1ml is 400 units to 80,000 units.
4. 4. The enzyme solution according to claim 1 to 3, Wherein the lipase is a lipase of one or more microorganisms selected from Pseudomonas cepacia (Pseudomonas Cepacia), rhizopus Oryzae (Rhizopus Oryzae), aspergillus niger (Aspergillus niger) and Aspergillus Oryzae (Aspergillus Oryzae).
5. 5. The enzyme solution according to claim 1 to 4, Wherein the cutinase is a cutinase of one or more microorganisms selected from the group consisting of Humicola insolens (Humicola Insolens), aspergillus oryzae, fusarium solani (Fusarium solani) and Pseudomonas putida (Pseudomonas putida).
6. 6. The enzyme solution according to claim 1 to 5, Wherein the lipase comprises a lipase of Pseudomonas cepacia, and The cutinase includes a humicola insolens cutinase.
7. 7. The enzyme solution according to claim 1 to 6, Lipase comprising Pseudomonas cepacia lipase and Rhizopus oryzae lipase as the lipase, and A cutinase comprising humicola insolens and a cutinase of aspergillus oryzae as the cutinase.
8. 8. The enzyme solution according to any one of claim 1 to 7, A lipase comprising Pseudomonas cepacia as said lipase, and A cutinase comprising humicola insolens and a cutinase of aspergillus oryzae as the cutinase.
9. 9. The enzyme solution according to claim 1 to 8, Also included are one or more enzymes selected from the group consisting of alpha-amylase, cellulase, proteinase K, proteolytic enzyme, acetyl-CoA carboxylase, and alkaline protease.
10. 10. A biodegradability assessment method for a biodegradable resin, comprising: i) Contacting an enzyme solution comprising a hydrolase enzyme and phosphate buffered saline with a biodegradable resin, the hydrolase enzyme comprising a lipase and a cutinase, and Ii) observing the degradation state of the biodegradable resin over time.
11. 11. The method for evaluating biodegradability of a biodegradable resin according to claim 10, Wherein i) is performed by dropping the enzyme solution onto a resin sample comprising a biodegradable resin.
12. 12. The method for evaluating biodegradability of a biodegradable resin according to claim 10, Wherein i) is performed by immersing a resin sample comprising a biodegradable resin in the enzyme solution.
13. 13. The method for evaluating biodegradability of a biodegradable resin according to claim 10, Wherein i) a sample is prepared by spraying a biodegradable resin solution onto a culture medium, and then dropping the enzyme solution onto the sample, and Ii) by observing the change over time of the transparent areas formed on the sample.
14. 14. The biodegradability assessment method for a biodegradable resin according to any one of claim 10 to 13, Wherein ii) comprises: a) Obtaining an image of the biodegradable resin according to the elapsed unit time, and B) Biodegradability was evaluated by the change of images of the resin sample with time.
15. 15. The method for evaluating biodegradability of a biodegradable resin according to claim 14, Wherein b) comprises: b-1) obtaining a difference in brightness from an image immediately after the dropwise addition of hydrolase for each image of the resin sample obtained from the elapsed unit time; b-2) representing the image brightness difference as a function of elapsed unit time, and B-3) assessing biodegradability by analyzing said function.
16. 16. The method for evaluating biodegradability of a biodegradable resin according to claim 15, Wherein b-3) the biodegradability of the biodegradable resin is evaluated by determining the time when the second derivative of the function is at a maximum.

Description

Enzyme solution and method for evaluating biodegradability of biodegradable resin using the same Technical Field Cross Reference to Related Applications The present application claims the benefit of priority based on korean patent application nos. 10-2024-01119112, 10-2024-0111913 and 10-2024-0111914, filed on 3 months 9 of 2024, and korean patent application No. 10-2024-0183657, filed on 11 months 12 of 2024, the entire contents of which are disclosed in the documents being incorporated as part of the present specification. The present disclosure relates to an enzyme solution having biodegradability of a biodegradable resin and useful for evaluating biodegradability of a biodegradable resin, and a biodegradability evaluation method for a biodegradable resin using the same. Background Conventional petroleum-based plastic materials do not naturally decompose and cause serious environmental pollution. Accordingly, biodegradable polymers such as Polylactide (PLA), poly (butylene adipate-co-terephthalate) (PBAT), and polybutylene succinate (PBS) that are biodegradable in nature are attracting attention as alternatives to petroleum-based plastic materials. In developing such biodegradable resins, biodegradability assessment is indispensable. However, the existing biodegradability test is performed for several weeks to several months according to various conditions such as soil, compost, or ocean, and there are problems in that expensive large-sized equipment is required. Therefore, it is required to develop an evaluation method capable of evaluating the biodegradability of a biodegradable resin in a short period of time using a simpler method. Disclosure of Invention Technical problem An object of the present invention is to provide an enzyme solution which has degradability to a biodegradable resin and can more simply and more rapidly predict biodegradability of the biodegradable resin when exposed to a natural environment. Furthermore, it is an object of the present invention to provide a biodegradability assessment method for a biodegradable resin using the enzyme solution. Technical proposal According to one embodiment of the present disclosure, an enzyme solution is provided comprising a hydrolase enzyme comprising a lipase and a cutinase, and phosphate buffered saline. According to another embodiment of the present disclosure, there is provided a biodegradability assessment method for a biodegradable resin, comprising the steps of i) contacting an enzyme solution comprising a hydrolase enzyme and phosphate buffered saline with the biodegradable resin, the hydrolase enzyme comprising a lipase and a cutinase, and ii) observing the degradation state of the biodegradable resin over time. Advantageous effects The enzyme solution of the present disclosure has biodegradability of a biodegradable resin and can be effectively used for evaluating biodegradability of a biodegradable resin. According to the biodegradability assessment method for a biodegradable resin using the enzyme solution of the present disclosure, biodegradability of a biodegradable resin can be assessed simply and rapidly without expensive large-scale equipment. Further, according to the biodegradability assessment method for a biodegradable resin, the biodegradability of the biodegradable resin under various natural environmental conditions such as soil, compost, and sea can be easily predicted. Drawings FIG. 1 shows the results of biodegradability evaluation in soil/compost environment of poly (butylene adipate-co-terephthalate) compound 1 (PBAT C1) and poly (butylene adipate-co-terephthalate) compound 2 (PBAT C2) performed in (3) of example A-1. FIG. 2 shows the results of biodegradability tests under industrial composting conditions measured according to ISO 14855-1 standard of PBAT C1, PBAT C2 and cellulose (manufacturer: sigma-Aldrich, product number: 310697). FIG. 3 shows the results of biodegradability evaluation in a marine environment of poly (butylene succinate) (PBS) and poly (butylene succinate adipate) (PBSA) performed in (3) of example A-2. Fig. 4 shows the biodegradability test results under marine conditions measured according to ASTM D6691 for PBS and PBSA. FIG. 5 shows the results of biodegradability evaluation of the PBAT/TPS compound and the PBAT/PLA compound in a soil/compost environment performed in (3) of example B-1. FIG. 6 shows the results of biodegradability assessment in soil environment according to ISO 17556 standard of the PBAT/TPS compound, PBAT/PLA compound and cellulose (manufacturer: sigma-Aldrich, product number: 310697) used in example B-1. FIG. 7 shows the results of biodegradability evaluation under marine conditions of PBS and PBSA performed in (3) of example B-2. FIG. 8 is a schematic view showing a method of dropping an enzyme solution onto a sample in the biodegradability evaluation of examples C-1 and C-2. FIG. 9 shows the results of biodegradability assessment in an industrial composting environment