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CN-122003608-A - Automatic analysis device and dispensing method thereof

CN122003608ACN 122003608 ACN122003608 ACN 122003608ACN-122003608-A

Abstract

The invention aims to provide an automatic analysis device capable of reducing residues among liquids and realizing high dispensing precision. The automatic analyzer of the present invention includes a dispensing mechanism that dispenses a sample stored in a sample container or a reagent stored in a reagent container into a reaction container by operating a syringe connected to a nozzle, and a control unit that controls the dispensing mechanism, wherein the control unit performs a discharge operation for operating the syringe in a discharge direction before the dispensing mechanism sucks the sample or the reagent and then discharges the sample or the reagent into the reaction container, the discharge operation including a first discharge operation performed in a state where the nozzle is positioned at a position of the sample container or the reagent container, and a second discharge operation performed in a middle of a movement of the nozzle from the position of the sample container or the reagent container to the position of the reaction container, and an operation amount of the syringe in the first discharge operation is smaller than an operation amount of the syringe in the second discharge operation.

Inventors

  • FUKAYA MASASHI

Assignees

  • 株式会社日立高新技术

Dates

Publication Date
20260508
Application Date
20240919
Priority Date
20231006

Claims (10)

  1. 1. An automatic analysis device is provided with: a dispensing mechanism for dispensing a sample contained in the sample container or a reagent contained in the reagent container into the reaction container by operating a syringe connected to the nozzle, and A control unit for controlling the dispensing mechanism, The control unit performs a discharge operation of operating the syringe in a discharge direction before the sample or the reagent is sucked by the dispensing mechanism and then discharged to the reaction container, It is characterized in that the method comprises the steps of, The discharging operation includes: a first discharging operation performed in a state where the nozzle is located at the position of the sample container or the reagent container, and A second discharging operation performed in the middle of the movement of the nozzle from the position of the sample container or the reagent container to the position of the reaction container, The amount of actuation of the syringe in the first discharge action is smaller than the amount of actuation of the syringe in the second discharge action.
  2. 2. The automatic analyzer according to claim 1, wherein, At least in the second discharging operation, the sample or the reagent is discharged from the nozzle.
  3. 3. The automatic analyzer according to claim 1, wherein, Before the end of the first discharge operation, the backlash of the syringe is removed, and the sample or the reagent is discharged from the nozzle from the start of the second discharge operation.
  4. 4. The automatic analyzer according to claim 1, wherein, At the end of the first discharge operation, a backlash of the syringe remains, the backlash is removed during the second discharge operation, and the sample or the reagent is discharged from the nozzle.
  5. 5. The automatic analyzer according to claim 1, wherein, The amount of actuation of the syringe in the first expelling action is zero.
  6. 6. The automatic analyzer according to claim 1, wherein, The automatic analyzer has a cleaning tank for cleaning the outer side of the nozzle, The second discharging operation is performed in a state where the nozzle is located at the position of the cleaning tank.
  7. 7. The automatic analyzer according to claim 6, wherein, In the second discharging operation, the cleaning water is in a state of striking the nozzle from the start of discharging the sample or the reagent to the end of discharging.
  8. 8. The automatic analyzer according to claim 6, wherein, In the second discharging action, the nozzle is stopped.
  9. 9. The automatic analyzer according to claim 1, wherein, The second discharge operation is constituted by a plurality of operations performed when the nozzle is located at different positions.
  10. 10. A dispensing method for dispensing a sample contained in a sample container or a reagent contained in a reagent container into a reaction container using an automatic analyzer comprising a dispensing mechanism having a nozzle and a syringe and a control unit for controlling the dispensing mechanism, The dispensing method comprises the following steps: a liquid sucking step in which the control unit causes the syringe to operate in a sucking direction in a state where the nozzle is positioned at the position of the sample container or the reagent container; A first discharge step in which the control unit operates the syringe in a discharge direction in a state in which the nozzle is positioned at the position of the sample container or the reagent container; A second discharging step in which the control unit operates the syringe in a discharging direction while the nozzle is moving from the position of the sample container or the reagent container to the position of the reaction container, and A liquid discharge step in which the control unit operates the syringe in a discharge direction in a state where the nozzle is positioned at the position of the reaction vessel, The amount of actuation of the syringe in the first expelling step is less than the amount of actuation of the syringe in the second expelling step.

Description

Automatic analysis device and dispensing method thereof Technical Field The present invention relates to an automatic analyzer and a dispensing method thereof. Background An automatic analyzer for analyzing a biological sample such as blood or urine includes a dispensing mechanism for dispensing a liquid containing a sample or a reagent into a reaction container. The dispensing mechanism sucks or discharges the liquid by operating a syringe connected to the nozzle. Here, since a gap (backlash) is present in the connecting portion of each mechanical member constituting the syringe, when the operation direction of the syringe is reversed, a minute idle operation due to the gap occurs. Therefore, for example, a syringe operation (backlash removal operation) for eliminating the idle rotation is required before the liquid sucked by the dispensing mechanism is discharged to the reaction vessel. For example, patent document 1 discloses that a sample probe is lowered at a position of a sample container to suck a sample in the sample container, and then a backlash removing operation is performed at the position of the sample container to discharge the sample from the sample probe (paragraph 0038, fig. 6, etc.). Prior art literature Patent literature Patent document 1 Japanese patent application laid-open No. 2019-174318 Disclosure of Invention Problems to be solved by the invention After the sample probe attracts the sample, when the syringe is moved in the discharge direction as a backlash removal operation, the idle rotation of the syringe is eliminated, and then the sample in the sample probe is discharged when the syringe is continued to be moved in the discharge direction. The sample discharged at this time is not only the sample to be dispensed this time, but also a small amount of the sample to be dispensed last time remains. Therefore, as in the technique described in patent document 1, even when the backlash removal operation after the sample probe is sucked by the sample probe is performed only when the sample probe is positioned in the sample container, the pre-sample returned to the sample container cannot be ignored. Further, since the pre-sample is returned to the sample container and accumulated each time dispensing is repeated, there is a possibility that the dispensing accuracy may be lowered. The present invention has been made in view of the above problems, and an object of the present invention is to provide an automatic analyzer and a dispensing method thereof, which reduce residues between liquids and achieve high dispensing accuracy. Means for solving the problems In order to achieve the above object, an automatic analyzer according to the present invention includes a dispensing mechanism that operates by a syringe connected to a nozzle and dispenses a sample stored in a sample container or a reagent stored in a reagent container into a reaction container, and a control unit that controls the dispensing mechanism, wherein the control unit performs a discharge operation for operating the syringe in a discharge direction before the dispensing mechanism sucks the sample or the reagent and then discharges the sample or the reagent into the reaction container, and the discharge operation includes a first discharge operation performed in a state where the nozzle is located at a position of the sample container or the reagent container, and a second discharge operation performed in a middle of a movement of the nozzle from the position of the sample container or the reagent container to a position of the reaction container, and an operation amount of the syringe in the first discharge operation is smaller than an operation amount of the syringe in the second discharge operation. Effects of the invention According to the present invention, it is possible to provide an automatic analyzer and a dispensing method thereof, which can reduce residues between liquids and achieve high dispensing accuracy. Drawings Fig. 1 is a perspective view schematically showing the overall structure of an automatic analyzer. Fig. 2 is a plan view schematically showing the main structure of the automatic analyzer. Fig. 3 is a diagram schematically showing a flow path structure of the sample dispensing mechanism. Fig. 4 is a diagram showing a sequence of sample dispensing operations in the automatic analyzer of the comparative example. Fig. 5 is a diagram showing a mechanism of residual precursor remaining inside the nozzle. Fig. 6 is a diagram showing a sequence of sample dispensing operations in the automatic analyzer of example 1. Fig. 7A is a table showing values of the first discharge operation amount and the second discharge operation amount set in comparative example and example 1. Fig. 7B is a graph showing the average value of the inter-sample residual ratios in comparative examples and example 1. Fig. 8 is a diagram showing a sequence of sample dispensing operations in the automatic analyzer of example 2. Fig. 9 is