CN-122003999-A - Application of graphene in improving species diversity of sorghum rhizosphere microbial community in saline-alkali soil

Abstract

The invention discloses application of graphene in improving species diversity of sorghum rhizosphere microbial communities in saline-alkali soil, and belongs to the technical field of crop ecological carbon sinks. According to the invention, by using the graphene sol fertilizer to treat sorghum crops in saline-alkali soil, the graphene is unexpectedly found to be capable of remarkably promoting the abundance and diversity of rhizosphere microbial community species of sorghum, and recruiting some potential plant probiotics in root systems. According to the invention, the amplicon sequencing method is used for classifying and counting the rhizosphere microbial community conditions of sorghum, and the fact that graphene promotes growth of sorghum in saline-alkali soil and depends on change of rhizosphere microbial community structures is proved, so that the application range of graphene is widened, and a theoretical foundation is laid for wide application of graphene in agriculture.

Inventors

• ZHAO JIANGUO
• CHEN XIAOKANG
• YANG JIASHENG
• SHEN LIJIA
• SHI WENTANG
• QIAO JUN
• CHEN ZHIWEN

Assignees

• 山西大同大学

Dates

Publication Date

20260512

Application Date

20241112

Claims (10)

1. 1. Use of graphene in improving species diversity of crop rhizosphere microbial communities.
2. 2. The use of claim 1, wherein the graphene is graphene oxide. Preferably, the graphene is applied to the soil in the form of a graphene sol fertilizer. Preferably, in the graphene sol fertilizer, the concentration of graphene is 5-20 mg/L.
3. 3. The use according to claim 1 or 2, wherein the graphene is prepared by two-step electrochemical methods using flexible graphite paper and platinum foil as cathode and anode respectively.
4. 4. Application of graphene in preparing crop growth fertilizer.
5. 5. The application of claim 4, wherein the concentration of graphene in the fertilizer is 5-20 mg/L.
6. 6. A fertilizer comprising the graphene of any one of claims 1-3.
7. 7. The fertilizer material of claim 6, wherein the concentration of graphene in the fertilizer material is 5-20 mg/L.
8. 8. Use of a fertilizer according to claim 6 or 7 for improving the diversity of crop rhizosphere microbial community species and/or for promoting crop growth.
9. 9. A method of improving the diversity of species of crop rhizosphere microbial communities comprising applying the graphene of any one of claims 1-3 and/or the fertilizer of claim 6 or 7 to crop growth soil.
10. 10. A method of promoting crop growth comprising applying the graphene of any one of claims 1-3 and/or the fertilizer of claim 6 or 7 to crop growth soil.

Description

Application of graphene in improving species diversity of sorghum rhizosphere microbial community in saline-alkali soil Technical Field The invention belongs to the technical field of ecological carbon sinks, and relates to application of graphene in improving species diversity of sorghum rhizosphere microbial communities in saline-alkali soil. Background Sorghum (Sorghum bicolor) is an important cereal crop that is widely planted in tropical and subtropical regions of the world. Sorghum has the characteristics of drought resistance, barren resistance and strong stress resistance, so that the sorghum becomes an important grain and feed source in areas where other crops grow under the climatic conditions. Saline-alkali soil contains a large amount of soluble saline-alkali substances, so that the content of organic matters in the soil is reduced, and the diversity of microorganisms is deficient, so that plants are difficult to grow. The physical and chemical properties of the soil can be improved by planting alkali-resistant crops and applying water-soluble fertilizer by using drip irrigation technology to increase the microbial diversity, thereby promoting the soil health and improving the productivity of crops (such as sorghum). Disclosure of Invention In order to improve the technical problems, the invention provides application of graphene in improving species diversity of crop rhizosphere microbial communities. Graphene (Graphene) is a nanomaterial with a number of unique properties including unique two-dimensional structure, ultra-large specific surface area, adjustable surface chemistry, and excellent electrical, thermal, optical, and mechanical properties. Soil microorganisms are an important component in the soil ecosystem, and are called a second set of genome of plants, and participate in various ecological processes such as organic matter decomposition, nutrient conversion circulation and the like in the soil. The graphene is used for improving the diversity of plant rhizosphere microbial communities, so that the soil health is effectively promoted, and the productivity of crops (such as sorghum) is improved. According to an embodiment of the invention, the crop is preferably a saline-alkali soil crop. For example, the crop is sorghum. According to the embodiment of the invention, the graphene is applied to improving the species diversity of rhizosphere microbial communities of crops in the saline-alkali soil, and is particularly used for improving the species diversity of rhizosphere microbial communities of sorghum crops in the saline-alkali soil. According to an embodiment of the invention, the graphene is graphene oxide. According to an embodiment of the invention, graphene is applied to saline-alkali soil in the form of graphene sol fertilizer. For example, graphene is dispersed in water to obtain a graphene sol fertilizer, which is then used in soil. Aiming at the composition and difference of the species of the rhizosphere microbial community of the sorghum in the saline-alkali soil, the invention classifies and counts the rhizosphere microbial community condition of the sorghum by using the graphene sol fertilizer to treat the sorghum crop in the saline-alkali soil, improves the microbial diversity of the sorghum crop in the saline-alkali soil in the North of Jinand proving, and confirms the application of the graphene in improving the microbial diversity of the rhizosphere of the sorghum in the saline-alkali soil. According to the embodiment of the invention, in the graphene sol fertilizer, the concentration of graphene is 5-20 mg/L, and is exemplified by 5mg/L, 8mg/L, 10mg/L, 15mg/L and 20mg/L. According to the embodiment of the invention, the graphene is prepared by taking flexible graphite paper and platinum foil as a cathode electrode and an anode electrode respectively through a two-step electrochemical method. In one embodiment of the present invention, in the two-step electrochemical method, the initial voltage of the first-step electrochemical method is, for example, 1.5 to 2.0V, and is, for example, 1.60V. In one embodiment of the present invention, in the two-step electrochemical method, the initial voltage of the second-step electrochemical method is, for example, 2.0 to 3.0V, and is, for example, 2.5V. In one embodiment of the present invention, the electrolyte of the second-step electrochemical process is a sulfuric acid solution in the two-step electrochemical process. For example, the concentration of the sulfuric acid solution is 40-60 wt.%, and exemplary is 50wt.%. In one embodiment of the present invention, the second electrochemical process comprises immobilizing the blue intermediate product obtained by the first electrochemical process with a platinum foil as a cathode electrode. According to an embodiment of the invention, the microbial community species comprises at least one of bacteria, fungi. For example, at least one of Pseudomonas (Pseudomonas), bacillus (Bacil