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CN-122004007-A - Mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment

CN122004007ACN 122004007 ACN122004007 ACN 122004007ACN-122004007-A

Abstract

The invention belongs to the technical field of seed cultivation, and particularly relates to a mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment. The invention provides a magnetic field-sodium selenite mung bean seed treatment method based on time sequence cooperation, which effectively solves the contradiction between efficiency and safety in selenium bioaugmentation. Firstly, the low-frequency rotating magnetic field pretreatment is used for inducing seeds to generate physiological pre-adaptation, activating stress response and metabolic pathways, creating a high-capacity buffer environment for the subsequent low-concentration sodium selenite treatment, thereby remarkably improving the selenium absorption and organic conversion efficiency, realizing high-efficiency selenium enrichment and simultaneously guaranteeing germination and seedling growth. Secondly, the system enhances the resistance of seedlings to drought, low temperature and other stress by cooperatively processing the defense network of the programmable plants. The whole treatment is completed before germination, the selenium is not needed to be applied in the field, the soil pollution is avoided, the treatment liquid can be recycled, the method has the characteristics of green, low consumption and controllability, and a new way is provided for crop nutrition enhancement.

Inventors

  • LIU XIAOMENG
  • YU JIANDONG
  • CHEN QIANGWEN
  • XU FENG
  • CHENG SHUIYUAN
  • Rao shen

Assignees

  • 武汉轻工大学
  • 湖北玉如意生物科技股份有限公司

Dates

Publication Date
20260512
Application Date
20260206

Claims (9)

  1. 1. A mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment is characterized by comprising the following steps: s1, seed pretreatment: healthy mung bean seeds are selected for cleaning and wetting treatment; S2, magnetic field pre-cooperative treatment: placing the wet seeds in a low-frequency rotating magnetic field for treatment; S3, seed soaking treatment of sodium selenite: Immersing the seeds treated in the step S2 into sodium selenite solution for soaking treatment; S4, magnetic field consolidation treatment: Placing the seeds treated and drained in the step S3 in a static magnetic field for treatment; s5, germination culture: And (3) germinating or sowing the seeds treated in the step (S4).
  2. 2. The method for cultivating mung bean seeds based on the synergistic treatment of a magnetic field and sodium selenite according to claim 1, wherein the wetting treatment in the step S1 is to make the water content of the seeds reach 45-50%.
  3. 3. The mung bean seed cultivation method based on the cooperative treatment of the magnetic field and the sodium selenite according to claim 1, wherein the frequency of the low-frequency rotating magnetic field is controlled to be 5-20 Hz, the magnetic field strength is 5-30 mT, the treatment time is 10-15 min, and the treatment temperature is 20-25 ℃ in the treatment in the step S2.
  4. 4. The method for cultivating mung bean seeds based on the synergistic treatment of a magnetic field and sodium selenite as claimed in claim 1, wherein the seeds are laid in a single layer in the magnetic field during the treatment in step S2.
  5. 5. The mung bean seed cultivation method based on the magnetic field and sodium selenite cooperative treatment according to claim 1, wherein the sodium selenite solution in the step S3 is prepared from analytically pure sodium selenite and deionized water with resistivity of not less than 18M Ω & cm, and the concentration of the prepared sodium selenite solution is 5-10 mg/L.
  6. 6. The method for cultivating mung bean seeds based on the synergistic treatment of a magnetic field and sodium selenite according to claim 1, wherein the soaking treatment time in the step S3 is 15-30 min, the temperature is 20-25 ℃, and after the soaking is finished, the mung bean seeds are immediately drained and the surfaces of the mung bean seeds are rinsed with deionized water.
  7. 7. The mung bean seed cultivation method based on the magnetic field and sodium selenite cooperative treatment according to claim 1, wherein the magnetic field strength of the static magnetic field treatment in the step S4 is 5-20 mT, the treatment time is 30-60 min, and the treatment temperature is 20-25 ℃.
  8. 8. The method for cultivating mung bean seeds based on the synergistic treatment of a magnetic field and sodium selenite according to claim 1, wherein the seeds treated in the static magnetic field in step S4 are laid flat in a single layer in the static magnetic field.
  9. 9. The method for cultivating mung bean seeds based on the synergistic treatment of magnetic field and sodium selenite according to claim 1, wherein the interval between the step S2 and the step S3 is not more than 30 min, and the interval between the step S3 and the step S4 is not more than 15 min.

Description

Mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment Technical Field The invention belongs to the technical field of seed cultivation, and particularly relates to a mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment. Background Selenium is a trace element necessary for human body, and has important physiological functions of resisting oxidation, enhancing immunity and the like. Cultivation of selenium-enriched agricultural products by agricultural bio-enhancement technology is an effective way to improve public selenium nutrition. Mung beans are an important legume crop, and selenium-rich cultivation of mung beans is of great concern. At present, mung bean selenium enrichment is mainly realized by foliage spraying or soil application of selenium fertilizer (such as sodium selenite). However, these methods have the following inherent drawbacks: (1) Selenium toxicity contradicts enrichment efficiency, high concentration selenium treatment can inhibit seed germination and seedling growth to produce toxicity, and low concentration selenium treatment has limited selenium enrichment effect. (2) The traditional method considers selenium as stress, plants can only passively tolerate, the treatment process does not actively activate or strengthen the stress resistance defense system of the plants, and selenium enrichment and stress resistance are difficult to be compatible. (3) The conversion rate of organic selenium is low, the conversion efficiency of inorganic selenium (sodium selenite) to organic selenium (such as seleno-amino acid) is unstable, and the organic selenium proportion in the product is low. (4) And the environmental risk is that the soil Shi Xiyi causes residue and ecological risk, and does not accord with the green agriculture concept. In recent years, there have been studies on attempts to treat seeds by physical means such as a magnetic field to promote germination or enhance resistance, and applications in which a magnetic field is simply combined with nutrient solution treatment. However, in the prior art, the magnetic field and the chemical treatment are regarded as independent and interchangeable factors to be simply overlapped, and the coupling effect of 1+1>2 cannot be realized due to the lack of time sequence design and synergistic mechanism based on plant physiological metabolic pathways, so that the contradiction can not be fundamentally solved. Therefore, a novel mung bean seed treatment method capable of cooperatively improving selenium-rich efficiency and seedling stress resistance, friendly to environment and definite in mechanism is developed, and the method has important practical significance. Disclosure of Invention The invention aims at solving the existing problems and provides a mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment. The invention is realized by the following technical scheme: a mung bean seed cultivation method based on magnetic field and sodium selenite cooperative treatment comprises the following steps: s1, seed pretreatment: healthy mung bean seeds with full particles and no mildew are selected, cleaned and drained by deionized water, and then wet treatment is carried out; S2, magnetic field pre-cooperative treatment: placing the wet seeds in a low-frequency rotating magnetic field for treatment; S3, seed soaking treatment of sodium selenite: Immersing the seeds treated in the step S2 into sodium selenite solution for soaking treatment; S4, magnetic field consolidation treatment: Placing the seeds treated and drained in the step S3 in a static magnetic field for treatment; s5, germination culture: And (3) germinating or sowing the seeds treated in the step (S4). Further, the moisture content of the seeds in the step S1 is 45-50%. Further, in the step S2, the frequency of the low-frequency rotating magnetic field is controlled to be 5-20 Hz, the magnetic field strength is 5-30 mT, the treatment time is 10-15 min, and the treatment temperature is 20-25 ℃; Further, the seeds are tiled in a single layer in the magnetic field during the treatment in step S2. Further, the sodium selenite solution in the step S3 is prepared from analytically pure sodium selenite and deionized water with resistivity not less than 18M omega cm, and the concentration of the prepared sodium selenite solution is 5-10 mg/L. Further, in the step S3, the soaking treatment is performed for 15-30 min at 20-25 ℃, and after the soaking is finished, the seeds are immediately drained and the surfaces of the seeds are briefly rinsed by a small amount of deionized water. Further, in the step S4, the magnetic field strength of the treatment in the static magnetic field is 5-20 mT, the treatment time is 30-60 min, and the treatment temperature is 20-25 ℃; Further, the seeds processed in the static magnetic field in step S4 are tiled in a single layer in the