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CN-122004011-A - Method for germinating sesbania seeds

CN122004011ACN 122004011 ACN122004011 ACN 122004011ACN-122004011-A

Abstract

The invention belongs to the technical field of agricultural biology, and particularly relates to a method for germinating sesbania seeds. Collecting mature sesbania seed, heat treating for 2 hr, grinding the damaged seed coat with fine sand, soaking in hydrogen peroxide and gibberellin, and final germinating in saturated sodium bicarbonate environment with micro ventilation holes in a temperature varying mode of 12 hr in light and 12 hr in dark. The method breaks the seed coat disorder through multi-step cooperative treatment, optimizes germination conditions, and can remarkably improve the germination efficiency of sesbania seeds.

Inventors

  • LU YUHUA
  • HU YIHONG
  • LIAO LIBING
  • YU DESHUI
  • ZHANG HUAJIE
  • WU NAN

Assignees

  • 江西省、中国科学院庐山植物园

Dates

Publication Date
20260512
Application Date
20260213

Claims (10)

  1. 1. A method of germinating sesbania seeds, comprising the steps of: S1, collecting mature sesbania angular fruits, and processing to obtain qualified seeds; S2, sequentially carrying out heat treatment and fine sand grinding on the qualified seeds in the step S1 to damage seed coats, and then carrying out continuous soaking treatment on hydrogen peroxide and gibberellin to obtain soaked sesbania seeds; s3, placing the soaked sesbania seeds obtained in the step S2 into a container containing saturated sodium bicarbonate, and culturing and germinating by adopting alternating temperature change of illumination and darkness; wherein the concentration of the hydrogen peroxide is 30% -50%, and the concentration of the gibberellin is 300-500mg/L.
  2. 2. The method for germination of sesbania seeds according to claim 1, wherein in step S1, the qualified seeds are obtained by naturally airing mature sesbania fruits to a water content of 2-3%, removing impurities after artificial threshing, and screening seeds with uniform particle size and no breakage.
  3. 3. The method for germination of sesbania seeds according to claim 1, characterized in that in step S2, the heat treatment step is to treat the qualified seeds for 2 hours in a constant temperature environment of 37-60 ℃, which is a water bath or a biochemical incubator.
  4. 4. The germination method of sesbania seeds according to claim 1, wherein in step S2, the fine sand grinding step comprises adding 1/4 volume of fine sand into a mortar, adding an equal volume of heat-treated seeds, and gently grinding for 2-5 minutes to obtain scratched seeds.
  5. 5. The germination method for sesbania seeds according to claim 4, wherein the grain size of the fine sand is 0.1-0.5mm, the surface scratches of the scratched seeds occupy half of the surface area of the seeds, and the ground surface has white marks.
  6. 6. The method for germination of sesbania seeds according to claim 4, wherein said scored seed has a embryo integrity of greater than or equal to 98%.
  7. 7. The method for germination of sesbania seeds according to claim 1, characterized in that in step S2, the soaking time of hydrogen peroxide is 2-4 hours, the temperature is 20-30 ℃, during which 2-3 times of gentle agitation are performed.
  8. 8. The method for germination of sesbania seeds according to claim 1, wherein in step S2, the gibberellin is soaked for 3-4 hours at 20-30 ℃.
  9. 9. The method for germination of sesbania seeds according to claim 1, wherein in step S3, the container containing saturated sodium bicarbonate is provided with a trace vent hole, and an open culture dish containing a reaction system of saturated sodium bicarbonate and diluted hydrochloric acid is arranged in the container.
  10. 10. The method for germination of sesbania seeds according to claim 1, characterized in that in step S3, the intensity of the illumination is 1400-1500lux, the cultivation time is 12 hours, the temperature is 15-35 ℃, the cultivation time in darkness is 12 hours, the temperature is 15-30 ℃.

Description

Method for germinating sesbania seeds Technical Field The invention belongs to the technical field of agricultural biology, and particularly relates to a method for germinating sesbania seeds. Background Sesbania is an annual herb of sesbania of leguminosae, and is widely distributed in tropical, subtropical and temperate areas, and natural growth or artificial cultivation records are available in the south provinces (areas) of Yangtze river areas of China. The sesbania has the core characteristics of strong ecological adaptability, high growth rate, outstanding nitrogen fixation capacity and the like, the root system of the sesbania can form a symbiotic system with rhizobium, the sesbania is an important biological fertilizer source in an agricultural ecological system, meanwhile, the sesbania stem and leaf is rich in crude protein (15% -22%) and dietary fiber, the sesbania stem and leaf can be used as a high-quality feed raw material, the oil content of seeds is about 10% -15%, the sesbania stem and leaf has industrial oil development potential, the sesbania stem and leaf is salt-tolerant, the sesbania stem and leaf can normally grow in strong alkaline soil with pH value of 10, even can normally grow in soil with salt content of 8g/kg, and the sesbania stem and leaf can tolerate short-term flooding stress. The plant is a preferable species for improving saline-alkali soil, repairing wetland and maintaining water and soil, and has irreplaceable effects in ecological restoration and agricultural sustainable development. In recent years, with the increasing prominence of global food safety and ecological environmental problems, the multi-dimensional utility value of sesbania has been refocused. In the field of agricultural production, sesbania can be used as green manure crops to effectively improve the content of soil organic matters, improve the physicochemical properties of the soil, reduce the environmental pressure caused by fertilizer application and accord with the green agricultural development concept, in the field of ecological restoration, the sesbania is resistant to abiotic stress such as saline alkali, drought, heavy metal and the like, a novel technical path is provided for ecological reconstruction of degraded lands, and in the aspect of molecular biology, the sesbania and rhizobium symbiotic nitrogen fixation mechanism, the metabolic regulation network of stress response and the like are researched on the basis of metabolic regulation and control, so that the sesbania and rhizobium are not only capable of providing references for functional gene excavation of leguminous plants, but also providing important gene resources for stress breeding and genetic improvement of crops. Although the application history of sesbania is long, the prior research still has the defect that the most important thing of obvious short plates is insufficient in researching the regulation mechanism of seed germination and dormancy of sesbania, and the problem of unstable germination rate caused by the dormancy characteristic of the seeds restricts the efficiency of large-scale cultivation and ecological restoration of sesbania. Therefore, the system research is developed aiming at the seed physiology aspect of sesbania, so that the blank of the basic biology research of leguminous plants can be filled, the core limiting factors for multipurpose development of the sesbania are clear, theoretical support and technical guidance can be provided for the large-scale application of the sesbania in the fields of green agriculture, ecological restoration and the like, and the system research has important theoretical significance and practical value for promoting the sustainable development of agriculture and improving the restoration efficiency of a degenerated ecological system. Aiming at the problems of low germination rate, nonuniform germination and the like of sesbania seeds, the invention provides a germination method of sesbania seeds, which is simple and convenient to operate, low in cost, high in flux, early in germination, short in germination passing time and high in germination rate by more than 80%. Provides a technical foundation for the large-scale propagation application and research of sesbania. Disclosure of Invention The application aims to overcome the defects in the prior art, and provides a method for germinating sesbania seeds, which comprises the following steps: S1, collecting mature sesbania angular fruits, and processing to obtain qualified seeds; S2, sequentially carrying out heat treatment and fine sand grinding on the qualified seeds in the step S1 to damage seed coats, and then carrying out continuous soaking treatment on hydrogen peroxide and gibberellin to obtain soaked sesbania seeds; s3, placing the soaked sesbania seeds obtained in the step S2 into a container containing saturated sodium bicarbonate, and culturing and germinating by adopting alternating temperature change of illumination