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CN-122004087-A - Golden fungus spore cultivation method based on single colony streak hybridization breeding

CN122004087ACN 122004087 ACN122004087 ACN 122004087ACN-122004087-A

Abstract

The invention provides a golden fungus spore cultivation method based on single colony streak hybridization breeding, and relates to the technical field of edible fungus cultivation. The breeding method comprises the steps of culturing the tremella aurantialba fruiting body in a suspension manner, after spores in the fruiting body are separated, transferring the spores into a flat culture medium to culture tremella aurantialba single colonies, randomly picking two tremella aurantialba single colonies, marking intersecting lines on the same clean flat culture medium to conduct hybridization culture, and simultaneously inoculating liquid strains of the tremella aurantialba as an auxiliary for fermentation, and uniformly smearing on the culture medium to induce tremella aurantialba. The breeding method solves the technical problems of complex operation, complicated steps, high requirements on equipment and long breeding time of the existing breeding method, and achieves the technical effects of high spore breeding speed, simple operation, low equipment requirements and capability of meeting the industrial production requirements.

Inventors

  • XU TIANXIU
  • FENG JIANFENG

Assignees

  • 东至河水山科技有限公司

Dates

Publication Date
20260512
Application Date
20240130
Priority Date
20231221

Claims (8)

  1. 1. A golden fungus spore cultivation method based on single colony streak hybridization breeding is characterized by comprising the following specific steps: s1, acquiring a golden fungus fruiting body block with a disinfected surface; s2, placing the culture flask filled with distilled water in a 121 ℃ high-temperature high-pressure sterilization; S3, clamping the tremella aurantialba fruiting body blocks, hanging the tremella aurantialba fruiting body blocks on hooks, hanging the tremella aurantialba fruiting body blocks in a culture bottle, culturing the tremella aurantialba fruiting body blocks at 20 ℃ in a dark place for 2 days, and collecting tremella aurantialba spores; s4, sucking distilled water containing spores at the bottom of the culture flask to a sterilized screening flat plate culture medium, and shaking to enable the distilled water to be uniformly distributed; S5, placing the screening plate culture medium at a light-shielding place at 20 ℃ for culturing for 4-5 days, and obtaining a plurality of macroscopic single colonies; S6, randomly picking two tremella aurantialba single colonies, marking out intersecting lines on the same sterilization tremella aurantialba flat plate culture medium for hybridization culture, simultaneously inoculating liquid strains of the tremella aurantialba, shaking uniformly on the tremella aurantialba flat plate culture medium, and inducing tremella aurantialba; S7.18-19 ℃ is cultivated for 25-30 days in a dark place, and the successful hybridization is obtained after young ear appears.
  2. 2. The method for cultivating golden fungus spores based on single colony streak hybridization breeding according to claim 1, wherein the screening plate medium in the step S4 is prepared from potato 200 g, glucose 20g, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g, peptone 1 g, yeast powder 1 g, agar 20g, water 1L and ampicillin 30 mg.
  3. 3. The method for cultivating golden fungus spores based on single colony streak hybridization breeding according to claim 1, wherein the culture medium formula of the fungus producing plate in the step S6 is potato 200 g, sucrose 20 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g, peptone 1 g, yeast powder 1 g, agar 20 g and water 1L.
  4. 4. The method for cultivating the golden fungus spores based on single colony streak hybridization breeding according to claim 1, wherein the preparation method of the liquid strain of the erigeron hirsutus in the step S6 is characterized by comprising the steps of 1) preparing and sterilizing a liquid culture medium, 2) placing the parent strain of the erigeron hirsutus, the sterilized liquid culture medium, an inoculating tool and the like into an ultra-clean workbench for ultraviolet sterilization for 15 minutes, 3) igniting an alcohol lamp, opening a parent strain flat plate in front of flame, cutting parent strain with a dissecting knife into parent strain with the size of 1cm 2 , placing the cut parent strain into the liquid culture medium, and 4) magnetically stirring and culturing the liquid strain for 5 days at 23 ℃ in a dark state.
  5. 5. The method for cultivating the golden fungus spores based on single colony streak hybridization breeding according to claim 4, wherein the liquid culture medium comprises glucose 20 g, yeast powder 3g, peptone 3g, potassium dihydrogen phosphate 1g and magnesium sulfate 1 g.
  6. 6. The method for cultivating golden fungus spores based on single colony streak hybridization as claimed in claim 1, wherein the disinfection method in step S1 is to uniformly spray alcohol on the surface of golden fungus fruiting body for disinfection.
  7. 7. The method for cultivating golden fungus spores based on single colony streak hybridization as claimed in claim 1, wherein the distilled water content in the step S2 is 20 mL, and the flask capacity is 500 mL.
  8. 8. The method for cultivating golden fungus spores based on single colony streak hybridization breeding according to claim 1, wherein the method for sterilizing the ultra-clean bench in step S3 is sterilizing by irradiating 15 min with ultraviolet lamp.

Description

Golden fungus spore cultivation method based on single colony streak hybridization breeding The application is a divisional application of the application application of which the application date is 2024, 01 and 30, the Chinese application number is 2024101332830 and the application name is 'golden fungus spore breeding method'. Technical Field The invention relates to the technical field of edible fungus cultivation, in particular to a golden fungus spore cultivation method based on single colony streak hybridization breeding. Background The tremella aurantialba is fungus of genus tremella Bao Geke. The basidiomycete fruit is large, hemispherical to amorphous and blocky, and the whole basidiomycete fruit is brain-shaped to split into a plurality of thick split petals with deep grooves, the diameter is 3-12 cm, and the height is 2-8 cm. The fruiting body layer is formed by exposing the surface layer, and is a wide loose area containing the basidiomycetes, which is from oval to oval, and from spherical to near spherical, rare pear shape with short stalk base, length of 13-25 micrometers, and width of 11.5-19.5 micrometers. The tremella aurantialba is distributed in high-altitude forest belts of Fujian, sichuan, yunnan, shanxi, guizhou, tibet and the like in China, and is produced in the northeast of Yunnan and Tibet. The plant is grown in summer and autumn, and grows more in the altitude of 1800-3200 m, and is often single or group-grown on broad-leaved wood such as Fagaceae and Betulaceae in broad-leaved forest and needle-broad mixed forest. The tremella aurantialba contains rich fat, protein and trace elements such as phosphorus, sulfur, manganese, iron, magnesium, calcium, potassium and the like, is a nutrition tonic, and can be used as a medicine. It has the actions of Wen Zhongdai cold in nature and sweet in taste, and can resolve phlegm, relieve cough and asthma, regulate qi, calm liver and intestine, and is mainly used for curing the diseases of lung heat, excessive phlegm, cold, cough, asthma and hypertension, etc. The tremella aurantialba has high edible and medicinal values, and the price of the wild tremella aurantialba is high and small, so that artificial cultivation is performed. The existing golden fungus breeding and cultivating technology generally adopts a single spore separation method, and the golden fungus spores are separated under a microscope, the method has high operation requirement, complex cultivating steps and long cultivating time, and cannot meet the actual production requirement, so the golden fungus spore cultivating method based on single colony streak hybridization is simple to operate and shorter in cultivating time. Disclosure of Invention (One) solving the technical problems Aiming at the defects of the prior art, the invention provides a golden fungus spore cultivation method based on single colony streak hybridization breeding, which solves the technical problems of high operation requirement, complex cultivation steps and long cultivation time of the existing cultivation method. (II) technical scheme In order to achieve the above purpose, the invention is realized by the following technical scheme: the golden fungus spore cultivation method based on single colony streak hybridization breeding comprises the following specific steps: s1, acquiring a golden fungus fruiting body block with a disinfected surface; s2, placing the culture flask filled with distilled water in a 121 ℃ high-temperature high-pressure sterilization; S3, clamping the tremella aurantialba fruiting body blocks, hanging the tremella aurantialba fruiting body blocks on hooks, hanging the tremella aurantialba fruiting body blocks in a culture bottle, culturing the tremella aurantialba fruiting body blocks at 20 ℃ in a dark place for 2 days, and collecting tremella aurantialba spores; s4, sucking distilled water containing spores at the bottom of the culture flask to a sterilized screening flat plate culture medium, and shaking to enable the distilled water to be uniformly distributed; S5, placing the screening plate culture medium at a light-shielding place at 20 ℃ for culturing for 4-5 days, and obtaining a plurality of macroscopic single colonies; S6, randomly picking two tremella aurantialba single colonies, marking out intersecting lines on the same sterilization tremella aurantialba flat plate culture medium for hybridization culture, simultaneously inoculating liquid strains of the tremella aurantialba, shaking uniformly on the tremella aurantialba flat plate culture medium, and inducing tremella aurantialba; S7.18-19 ℃ is cultivated for 25-30 days in a dark place, and the successful hybridization is obtained after young ear appears. Preferably, the screening plate medium in the step S4 is prepared from potato 200 g, glucose 20 g, potassium dihydrogen phosphate 1g, magnesium sulfate 1g, peptone 1g, yeast powder 1g, agar 20 g, water 1L and ampicillin 30 mg. Preferably, the formula of the ear-out