CN-122004098-A - Method for improving drought tolerance of tomatoes based on arbuscular mycorrhizal fungi

Abstract

The invention discloses a method for improving drought tolerance of tomatoes based on arbuscular mycorrhizal fungi, and belongs to the technical field of agricultural microorganisms. The invention realizes the enhancement of tomato drought tolerance by applying the preparation containing arbuscular mycorrhizal fungi, wherein the arbuscular mycorrhizal fungi are the special-shaped rhizopus, the active ingredients in the preparation exist in the forms of spores, hyphae, infected root sections and culture matrixes, each 40g of the preparation contains at least 100 special-shaped rhizopus spores, and carrier auxiliary materials can be added to improve the stability. The preparation is applied to root system of tomato when transplanting, and 40g of the preparation is applied to each plant. The method disclosed by the invention has the advantages of simplicity and convenience in operation, environment friendliness, low cost and wide applicability, can be used for cultivating and popularizing in fields or facilities, and has great significance in guaranteeing the yield and quality of tomatoes.

Inventors

• RUI WENJING
• JIA JIANMING
• DU JIANGTAO
• LI JING
• ZHANG JUNFENG
• ZHANG JING
• ZHANG XIAODAN
• WANG CHENG

Assignees

• 甘肃农业大学

Dates

Publication Date

20260512

Application Date

20260121

Claims (9)

1. 1. Use of arbuscular mycorrhizal fungi for improving drought tolerance of tomatoes.
2. 2. The application of arbuscular mycorrhizal fungi in preparing a preparation for improving drought tolerance of tomatoes.
3. 3. The use according to claim 1 or 2, wherein the arbuscular mycorrhizal fungus is rhizopus rotundus.
4. 4. A preparation for improving drought tolerance of tomatoes, which is characterized in that an active ingredient is arbuscular mycorrhizal fungi, and the active ingredient exists in the form of spores, hyphae, infected root segments and culture medium.
5. 5. The formulation of claim 4, further comprising an adjuvant, wherein the adjuvant is a carrier adjuvant.
6. 6. The formulation according to claim 4 or 5, wherein at least 100 oocyst is shaped per 40g of the formulation.
7. 7. A method for improving drought tolerance of tomatoes, comprising the step of applying to tomatoes a formulation according to any one of claims 4 to 6.
8. 8. The method of claim 7, wherein the formulation is applied at the time of tomato transplantation at the locus of the root system of the tomato.
9. 9. The method according to claim 7 or 8, wherein said formulation is administered at a dose of 40g of said formulation per tomato plant.

Description

Method for improving drought tolerance of tomatoes based on arbuscular mycorrhizal fungi Technical Field The invention relates to the technical field of agricultural microorganisms, in particular to a method for improving drought tolerance of tomatoes based on arbuscular mycorrhizal fungi. Background Drought stress is one of the major abiotic stresses affecting global agricultural production, severely limiting the growth, development and yield formation of vegetable crops. Tomato (Solanum lycopersicum) is a high-value vegetable widely planted worldwide, is rich in vitamins and minerals, and stands out in agricultural economy. However, the tomato has shallow root system and strong transpiration, is extremely sensitive to drought, and has the defects of disordered growth process, short plant, wilting leaves and greatly reduced photosynthetic efficiency under drought stress, and finally causes poor fruit development, sharp reduction of yield and reduced quality, thereby seriously affecting the production benefit. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi AMF) are a class of beneficial microorganisms in soil that can infect plant roots and establish symbiotic relationships with more than 80% of terrestrial plants. Research has proved that after AM fungi and plant root system form symbiont, the external hypha of root can help plant root system absorb water and nutrient in the area beyond the root system, improve the absorption of water and nutrient by plant, strengthen the stress resistance of plant. After symbiotic with AM fungi, plants can be effectively enhanced for drought stress tolerance by two approaches. One is direct action, namely, the AM fungus directly absorbs moisture through the outer hypha of the root of the AM fungus, improves the moisture condition of plants and improves drought resistance of the plants, the other is indirect action, namely, the AM fungus improves the root system configuration of the plants, enhances the absorption of mineral elements, improves photosynthesis of the plants and reduces oxidative damage of the plants by promoting the formation of soil aggregate structures, and in addition, the AM fungus can enhance the osmotic regulation capability of the plants, promote the plants to synthesize and accumulate more osmotic regulation substances such as proline, soluble sugar, betaine and the like, reduce the osmotic potential of cells and enhance the water retention capability of the cells. AM fungi can induce a series of gene expression related to drought resistance in plants through a signal transduction path, regulate the physiological and biochemical processes of the plants, and enhance the adaptability of the plants to drought stress. The former research shows that, under drought stress, compared with the AM fungus not inoculated, the AM fungus inoculated remarkably improves the water utilization rate of alfalfa, elm seedlings, oat, leymus chinensis and other seedlings. In vegetable crops, the alleviating effect of AM fungi on drought stress is also studied, for example, the inoculation of AM fungi can obviously improve the dry weight of the overground parts of onions under drought stress and promote the growth of the solanaceous vegetables. The northwest region of China is a arid and semiarid region, the region has less annual rainfall, the surface water resource is deficient, and the arid is an important factor affecting the development of the tomato industry in the region. Therefore, the method has important significance in exploring the influence of mycorrhizal symbiosis on the growth of tomatoes under drought stress and providing a theoretical basis for improving the drought resistance of tomatoes under drought environment. Disclosure of Invention The invention aims to overcome the defects and shortcomings of the prior art and provide a method for improving the drought tolerance of tomatoes based on arbuscular mycorrhizal fungi, which aims to break through the limitation that chemical regulation is easy to leave residues in the traditional tomato drought tolerance technology and realize the green and efficient improvement of the drought tolerance of tomatoes under drought stress. The invention provides an application of arbuscular mycorrhizal fungi in improving drought tolerance of tomatoes. The invention also provides application of arbuscular mycorrhizal fungi in preparation of a preparation for improving drought resistance of tomatoes. Further, the arbuscular mycorrhizal fungi are rhizopus heteromorphic. The invention also provides a preparation for improving drought tolerance of tomatoes, which is characterized in that the active ingredient is arbuscular mycorrhizal fungi, and the active ingredient exists in the form of spores, root sections infected by hyphae and culture matrixes. Further, the preparation also comprises auxiliary materials. Further, at least 100 oocyst dysmorphis spores per 40g of the formulation. The invention also provides a metho