CN-122004099-A - Application of yellow microsporium fungus in promoting growth of Solanaceae plants

CN122004099ACN 122004099 ACN122004099 ACN 122004099ACN-122004099-A

Abstract

The application of yellow microsporidian fungus in promoting the growth of Solanaceae plant belongs to the field of microbial technology. In order to solve the technical problems of narrow host range, dependence on living spores and the like in the prior art about the application of the yellow microsporidian fungi in promoting growth, the invention utilizes the yellow microsporidian fungi spores and fermentation supernatant to treat tomato seeds, benshi tobacco seeds and seedlings respectively, and discovers that the tomato seeds are coated by seed coating liquid containing the yellow microsporidian fungi spores, or tomato seedlings can be promoted to grow by irrigating tomato seedlings by using the yellow microsporidian fungi fermentation supernatant, seed soaking is carried out on Benshi tobacco seeds by using the yellow microsporidian fungi spore suspension, or Benshi tobacco seeds are coated by using seed coating liquid containing the yellow microsporidian fungi spores, or Benshi tobacco seedlings can be promoted to grow by irrigating the yellow microsporidian fungi fermentation supernatant. The invention lays a theoretical foundation for the subsequent deep development and utilization of the yellow microsporidian fungus as the plant probiotics.

Inventors

XU LIJIAN
WANG RUN
YANG XURAN
ZHANG DONGYE
JIANG ZIZHU

Assignees

黑龙江大学

Dates

Publication Date: 20260512
Application Date: 20260126

Claims (10)

1. Use of a spore suspension of a fungus of the genus yellow microsporidian, wherein the fungus of the genus yellow microsporidian is a Microsporum albus (Parametarhizium changbaiense) or Microsporum dahuricum (Parametarhizium hingganense), for promoting the growth of a plant of the family solanaceae, including tomato and Nicotiana benthamiana.
2. The use according to claim 1, wherein the use is to coat tomato seeds or tobacco seeds of the genus xanthomonas by preparing a seed coating solution from a spore suspension of the fungus.
3. The use according to claim 2, characterized in that the concentration of the spore suspension is 1 x 10 6 /mL to 1 x 10 7 /mL.
4. The use according to claim 3, characterized in that the concentration of the spore suspension is 1 x 10 6 /mL.
5. The use according to claim 2, wherein the seed coating solution further comprises a film forming agent.
6. The use according to claim 1, characterized in that it consists in seed-soaking tobacco seeds with a spore suspension of a fungus of the genus chrysosporium.
7. The use according to claim 6, wherein the spore suspension has a concentration of 1 x 10 5 /mL to 1 x 10 8 /mL.
8. The use of a fermentation supernatant of a micro-spore fungus in promoting growth of a plant of the family solanaceae, comprising tomato and nicotiana benthamiana, wherein the micro-spore fungus is micro-spore fungus albophora (Parametarhizium changbaiense) or micro-spore fungus axganensis (Parametarhizium hingganense).
9. The use according to claim 8, characterized in that it consists in watering tomato seedlings or tobacco seedlings with fermentation supernatants of fungi of the genus chrysosporium.
10. The use according to claim 8, wherein when the plant of the family Solanaceae is tomato, the fermentation supernatant is a 10% or 20% dilution of the fermentation supernatant, and when the plant of the family Solanaceae is Nicotiana benthamiana, the fermentation supernatant is a Microsporum dahuricum fermentation supernatant stock.

Description

Application of yellow microsporium fungus in promoting growth of Solanaceae plants Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to application of yellow microsporidian fungi in promoting growth of solanaceae plants. Background The genus Cladosporium (Parametarhizium) is a genus of Clavipitaceae (CLAVICIPITACEAE) established in 2021. Yellow microsporidian fungi are a class of microorganisms with potential plant growth promoting functions. Research has reported that spores of the plant can establish symbiotic relation with root systems of leguminous plants (such as mung beans and beans) and gramineous plants (such as rice and corn), so that the plant growth and development of the host plant are effectively promoted, and the plant spore has good potential as a biological fertilizer or a plant growth-promoting microbial inoculum. However, there are significant limitations and gaps in the current research: First, the host range is narrow and the pro-active effect of important genus plants is unknown. At present, research is focused on leguminous plants and gramineous plants, and systematic research on whether solanaceae plants (such as tomatoes and Nicotiana benthamiana) which are widely planted worldwide and have extremely high economic values can be symbiotic with yellow microsporidian fungi and can obtain growth promotion is not yet available. Tomato is one of the most important vegetable crops worldwide, nicotiana benthamiana is a key mode plant for botanic research, and it is clear that the yellow microsporidian fungi have important theoretical and application values on their promotion effect. Second, the optimal seed concentration for small seed is not yet defined. The size and weight of plant seeds significantly affect the manner in which they interact with microorganisms. Compared to the leguminous and gramineous crops studied, the seeds of tomato and tobacco are smaller and lighter, and their optimal fungal spore inoculation concentrations may be quite different. Dose optimization research is not performed on the characteristics of small seeds in the prior art, which restricts the effective application of the technology to solanaceae crops. Third, the prior art relies on living spores and the production presents a bottleneck. The currently accepted ingredient with growth promoting activity is washed eukaryotic spores. However, the living microbial preparation has the inherent defects of unstable biological activity, easiness in being influenced by environmental factors (temperature, humidity, pH and the like), short shelf life, high scale production cost and the like, and the commercialized development and field popularization of the yellow microsporidian fungus product are seriously limited. Fourth, the mechanism of growth promotion is unknown, and the activity of metabolites is urgently sought. The specific mechanism of action of the yellow microspora fungi in promoting plant growth has not been elucidated. A key scientific problem ensues: whether the growth promoting effect is completely dependent on direct symbiotic interaction of living bacteria and plants or whether metabolites produced during fermentation have biological activity as well is achieved if fermentation broths without living cells (spores) have growth promoting function as well, the novel stable preparation based on the metabolite is hopeful to be developed, so that the problems of instability, short shelf life and high cost of the living body preparation are fundamentally overcome, and the innovation of the technical path is realized. Therefore, aiming at the technical blank, the invention aims at systematically exploring 1) the growth promoting effect of the yellow microsporidian fungus on two important solanaceae plants of tomato and Nicotiana benthamiana, 2) the optimal spore inoculation concentration aiming at the characteristics of small seed of the yellow microsporidian fungus, and 3) the more critical is to evaluate whether the sterile fermentation filtrate (i.e. without any spores or living cells) of the yellow microsporidian fungus has independent plant growth promoting activity for the first time. The research provides important theoretical basis and technical basis for developing novel, stable and efficient plant pro-production products based on microbial metabolites. Disclosure of Invention In order to solve the defects and blank existing in the application of the yellow microsporium fungi, the invention utilizes the yellow microsporium fungi spores and fermentation supernatant (namely, no fungal cells only contain fungi metabolites) to treat tomato seeds, benshi tobacco seeds and seedlings respectively, and finds that the tomato seeds are coated by seed coating liquid containing the yellow microsporium fungi spores or the tomato seedlings are irrigated by the yellow microsporium fungi fermentation supernatant to promote the tomato growth, the Benshi tobacco seed