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CN-122004201-A - Diptera insect genitalia treatment device and method based on metal bath

CN122004201ACN 122004201 ACN122004201 ACN 122004201ACN-122004201-A

Abstract

The invention relates to the technical field of manufacturing of insect specimens, in particular to a diptera insect genital treatment device based on metal bath, which comprises a metal bath heater and a centrifuge tube, wherein the top of the metal bath heater is detachably connected with a metal block, the top of the metal block is provided with a plurality of placement holes, the metal block is detachably connected with a protective cover, the middle part of the protective cover is in sliding fit with a pressing plate, a plurality of first springs are arranged between the pressing plate and the inner top wall of the protective cover, the top of the pressing plate is fixedly connected with symmetrically arranged wedge-shaped blocks, the side walls of the wedge-shaped blocks are contacted with the inner side walls of the corresponding protective cover, the distance between the inclined surfaces of the wedge-shaped blocks, which are close to one side, is gradually increased upwards, the center of the inner top wall of the protective cover is fixedly connected with a fixed column, and a plurality of pushing components are arranged on the fixed column. According to the invention, the self-adaptive compaction of the centrifugal tube is combined by the metal bath heater, and the biptera insect genitalia is rapidly treated under the conditions of corresponding temperature and rotating speed, so that the efficient softening is realized and the structural damage is reduced.

Inventors

  • LIU RUOSI
  • ZHANG LIJIE
  • HUANG JING
  • DUAN FEIFEI
  • ZHANG BOYA

Assignees

  • 中国海关科学技术研究中心
  • 海关总署国际检验检疫标准与技术法规研究中心

Dates

Publication Date
20260512
Application Date
20260202

Claims (10)

  1. 1. The utility model provides a diptera insect genitalia processing apparatus based on metal bath, including metal bath heater (1) and centrifuging tube (13), metal bath heater (1) top is dismantled and is connected with metal piece (2), it has a plurality of settling holes (3) to open at metal piece (2) top, can dismantle on metal piece (2) and be connected with protective cover (4), a serial communication port: protective cover (4) middle part sliding fit has clamp plate (5), clamp plate (5) are parallel with protective cover (4) roof, be equipped with a plurality of first springs (6) between clamp plate (5) and protective cover (4) inner roof, first spring (6) both ends all with protective cover (4) inner roof and clamp plate (5) top fixed connection respectively, wedge (7) that clamp plate (5) top fixed connection had symmetrical setting, wedge (7) lateral wall all contacts with corresponding protective cover (4) inside wall, the inclined plane of wedge (7) is close to one side interval upwards increase gradually, top wall center fixedly connected with fixed column (8) in protective cover (4), be equipped with on fixed column (8) with wedge (7) respectively to be used for exerting to control wedge (7) to push away to push plate (5) to push force.
  2. 2. The diptera insect genital organ treatment device based on the metal bath according to claim 1, wherein the pushing components comprise telescopic barrels (9), one ends of the telescopic barrels (9) are fixedly connected with the side walls of the fixed columns (8), the other ends of the telescopic barrels (9) are fixedly connected with pushing blocks (10), the inner walls of the telescopic barrels (9) are fixedly connected with second springs (11), the other ends of the second springs (11) are fixedly connected with one sides, close to the fixed columns (8), of the corresponding pushing blocks (10), and the wedge-shaped blocks (7) are located in the movement tracks of the corresponding pushing blocks (10).
  3. 3. The diptera insect genital treatment device based on a metal bath according to claim 2, characterized in that the push blocks (10) are each provided with a rounded corner structure.
  4. 4. A diptera insect genital treatment device based on a metal bath according to claim 3, characterized in that both the pusher (10) and the wedge (7) surfaces are provided with a polytetrafluoroethylene coating.
  5. 5. The diptera insect genitalia treatment device based on the metal bath according to claim 1, wherein a silica gel plate (12) is fixedly connected to the bottom of the pressing plate (5).
  6. 6. The metal bath-based diptera insect genital treatment device according to claim 1, wherein the inner walls of the placement holes (3) are provided with heat-conducting silica gel layers.
  7. 7. A method of treating a diptera insect genital organ based on a metal bath, operating based on the structure of a diptera insect genital organ treating device based on a metal bath according to any one of claims 1-6, characterized by comprising the steps of: s1, sample preparation, namely taking a part containing the abdominal tail from a diptera insect specimen, dissecting by using a specimen needle, inverting the insect body during dissection, enabling the tail end of the abdominal to be visible, and placing the dissected sample into a centrifuge tube (13); S2, adding a treatment liquid, namely dropwise adding 0.2mL of alkaline tissue softening liquid into the centrifuge tube (13), and immersing a sample in the liquid; s3, metal bath heat treatment, namely placing a centrifugal tube (13) filled with a sample into a metal bath heater (1) preheated to a target temperature for heat treatment, driving a metal block (2) to do circular motion through the metal bath heater (1) so as to oscillate the centrifugal tube (13), ensuring uniform temperature in the centrifugal tube (13), simultaneously driving a protective cover (4) to do circular motion synchronously by the metal block (2), and driving a pressing plate (5) to downwards move by utilizing centrifugal force generated by rotation of the protective cover (4) so as to apply pressure to the top of the centrifugal tube (13) to prevent softening liquid in the centrifugal tube (13) from spilling out; s4, cooling and cleaning, namely immediately taking out the centrifuge tube (13) after the treatment is finished, naturally cooling or placing the centrifuge tube on a cold plate for short cooling, and carrying out short cleaning by using distilled water or other buffer solutions to neutralize residual alkali liquor; S5, tabletting, namely dripping a drop of glycerol on a glass slide, and placing the treated sample into the slide for temporary observation.
  8. 8. A diptera insect genital treatment device based on a metal bath according to claim 7, characterized in that the alkaline tissue softening solution in S2 is preferably a saturated potassium hydroxide solution.
  9. 9. A metal bath-based diptera insect genital treatment method according to claim 7, characterized in that the target temperature in S3 is 80 to 95 ℃, preferably 90 ℃, and the treatment time is 10 to 15 minutes, preferably 10 to 12 minutes.
  10. 10. A metal bath-based diptera insect genital treatment method according to claim 7, characterized in that the rotational speed of the metal bath heater (1) in S3 is 200rpm.

Description

Diptera insect genitalia treatment device and method based on metal bath Technical Field The invention relates to the technical field of manufacturing of entomology specimens, in particular to a diptera insect genitalia treatment device and method based on a metal bath. Background The genital structure of dipteran insects (mosquitoes, flies, tabanus, etc.) is one of the most critical morphological criteria for species identification. However, the genitals of diptera insects are usually contracted in the abdominal end and are wrapped by hard chitin shells and soft tissues, the preparation difficulty of dissection is great, in order to clearly observe and research the fine structures of the genitals, the hard shells and soft tissues need to be softened by a chemical method, so that the genitals are transparent or easy to dissect, the conventional treatment methods at present mainly comprise a sodium hydroxide (NAOH) solution boiling or cold soaking method, a lactic acid solution soaking method and the like, and the tissues wrapping the genitals are gradually decomposed or softened by utilizing the corrosiveness or permeability of the chemical reagents, so that the internal structures of the genitals are exposed, and the subsequent film-making observation and classification identification are convenient. The most commonly used method is a sodium hydroxide (NAOH) solution boiling method, a water bath is utilized to heat and promote softening of a sample in a centrifuge tube, but the treatment time of the water bath usually needs to be several hours to several days, in the process, the sample needs to be continuously placed in a strong alkali or strong acid reagent for several hours to several days, the corrosiveness of the strong alkali or strong acid reagent on chitin in the sample causes the destruction of fine structures (such as teeth, bristles and membranous parts on a bone piece) of the sample, and the water bath and the oil bath can only heat the sample, and the sample can sink into the bottom of the centrifuge tube in the heating process, so that the partial reflection of the sample is uneven; In the practical use process, a centrifugal tube with a built-in sample is placed in a fixing hole, the sample is heated and oscillated through an HCM100-Pro constant-temperature oscillation metal bath to ensure that the sample is heated uniformly, but when the centrifugal tube oscillates along a metal block in a circular motion, softened liquid in the centrifugal tube moves upwards along the wall of the centrifugal tube under the action of centrifugal force, when an operator carelessly does not completely close a centrifugal tube cover (a gap exists in the centrifugal tube cover), the softened liquid overflows from the gap under the action of centrifugal force, particularly when the rotating speed is high, the risk of liquid splashing is increased, in addition, the centrifugal tube can slightly shift due to vibration or centrifugal force in the oscillation process to further enlarge the gap of the centrifugal tube cover, the problem of liquid leakage is aggravated, the liquid in the centrifugal tube cannot be completely immersed due to leakage of the softened liquid, and the treatment effect is reduced. Accordingly, the present invention provides a device and a method for treating genitals of diptera based on a metal bath, so as to solve the above-mentioned problems. Disclosure of Invention In order to solve the problems, the invention provides a diptera insect genitalia treatment device and a diptera insect genitalia treatment method based on a metal bath, which are used for rapidly treating the diptera insect genitalia under the conditions of corresponding temperature and rotating speed by combining a metal bath heater with self-adaptive compaction of a centrifuge tube, so that high-efficiency softening is realized and structural damage is reduced. In order to achieve the purpose, the technical scheme is that the diptera insect genitalia treatment device based on the metal bath comprises a metal bath heater, wherein the top of the metal bath heater is detachably connected with a metal block, the top of the metal block is provided with a plurality of arrangement holes, the metal block is detachably connected with a protective cover, the middle part of the protective cover is in sliding fit with a pressing plate, the pressing plate is parallel to the top wall of the protective cover, a plurality of first springs are arranged between the pressing plate and the inner top wall of the protective cover, two ends of each first spring are respectively and fixedly connected with the inner top wall of the protective cover and the top of the pressing plate, the top of the pressing plate is fixedly connected with symmetrically arranged wedge blocks, the side walls of the wedge blocks are respectively contacted with the inner side walls of the corresponding protective cover, the distance between the inclined surfaces of the