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CN-122004214-A - Application of phenylacetaldehyde in improving rice blast resistance of rice

CN122004214ACN 122004214 ACN122004214 ACN 122004214ACN-122004214-A

Abstract

The invention belongs to the technical field of biological control, and provides application of phenylacetaldehyde in improving rice blast resistance, wherein the application is that the phenylacetaldehyde is used as a small molecule chemotactic agent for improving the rice blast resistance, and the effect of the phenylacetaldehyde on attracting bacillus and further inhibiting rice blast bacteria is evaluated.

Inventors

  • SU PIN
  • KANG HOUXIANG
  • ZHANG DEYONG
  • PENG WEIYE
  • Peng Qianze
  • GAO LIMING

Assignees

  • 岳麓山实验室

Dates

Publication Date
20260512
Application Date
20260122

Claims (7)

  1. 1. Use of phenylacetaldehyde for improving rice blast resistance.
  2. 2. The use according to claim 1, wherein phenylacetaldehyde is used for guiding bacillus to migrate to the phyllosphere and form a biofilm, and wherein said bacillus induces up-regulation of rice ISR marker genes OsPR2, osppr 1.
  3. 3. The use according to claim 1, wherein the phenylacetaldehyde is used in a concentration of 1 mg/L.
  4. 4. The use according to claim 1, wherein the phenylacetaldehyde is used in one or more of seed coating, rhizosphere drip irrigation, foliar spray.
  5. 5. The use according to claim 1, wherein the use is based on bacillus relative abundance or chemotactic index as a method for evaluating the efficacy of phenylacetaldehyde chemotactic agent.
  6. 6. A reagent for combating rice blast, wherein said reagent is prepared with phenylacetaldehyde as the sole active ingredient or in combination with a non-active ingredient.
  7. 7. The use of the agent according to claim 6 for rice blast resistance.

Description

Application of phenylacetaldehyde in improving rice blast resistance of rice Technical Field The invention belongs to the technical field of biological control, relates to control of rice blast, and in particular relates to application of phenylacetaldehyde in improving rice blast resistance. Background The blast is caused by Pyricularia oryzae (Magnaporthe oryzae) belonging to the family Clavipitaceae, ascomycetes, and is the first major disease in rice production. The pathogenic bacteria can generate mechanical pressure of 8 MPa to penetrate the rice epidermis through forming attachment cells, and the annual production of the global rice is reduced by 10-30%, and the direct economic loss exceeds 500 hundred million dollars. At present, chemical bactericides such as tricyclazole, isoprothiolane and the like are mainly relied on for prevention and treatment, but long-term application leads to M.oryzae to detect field resistance strains (drug resistance index > 10) of QoIs and DMIs medicaments, and simultaneously causes environmental problems such as excessive propiconazole residue (> 0.1 mg/kg) in rice, unbalanced microbial communities of a paddy field ecological system (40-60% reduction of beneficial bacteria abundance) and the like. Bacillus spp is recognized as an ideal biocontrol strain because it produces antibiotic lipopeptides such as surface active agents (Surfactin), fengycin (Fengycin) and Iturin (Iturin) and induces rice system resistance (ISR) by activating the jasmonic acid/ethylene signaling pathway. However, the field application faces two major core bottlenecks, namely firstly that the breeding amount of bacillus in the rice She Jiding is only 10 3-104 CFU/g blade in a natural state and is far lower than an effective bacteriostasis threshold value (10 6 CFU/g), secondly that the survival rate of the bacillus is less than 15% due to the ultraviolet radiation, dry-wet alternation and nutrition deficiency of the blade surface, and the biological control effect is unstable (the control effect variation coefficient CV is more than 35%), so that the industrialized application is severely restricted. Plant Volatile Organic Compounds (VOCs) -mediated assembly of rhizosphere/phyllospheric microorganisms is a recent research hotspot. VOCs such as 2, 4-di-tert-butylphenol secreted by rice root systems and cis-3-hexenol released by leaves have been demonstrated to specifically activate a bacillus CheAY two-component system through methyl receptor chemotactic proteins (MCPs) to enable the bacillus CheAY two-component system to directionally migrate and initiate expression of a biofilm-forming gene (sinI, epsA-O). However, the prior art has three defects of (1) complex components of natural VOCs, low effective concentration (only 0.1-1 mug/g fresh weight), difficulty in standardized production, (2) short half-life (< 24 h) and easiness in degradation of small molecule chemotactic agents such as malic acid and succinic acid which can recruit bacillus, and (3) no report of mass production, low cost and high stability of synthetic small molecule chemotactic agents for directionally recruiting bacillus in fields to prevent rice blast. Phenylacetaldehyde (PHENYLACETALDEHYDE, C 8H8 O, molecular weight 120.15) is a core volatile product of a phenylpropane metabolic pathway, is widely present in plant volatiles (5-15% in ratio) such as tea trees, tomatoes and the like and honey (content 1.2-3.5 mg/kg), and has a remarkable attracting effect on pollinating insects such as bees and the like. The physical and chemical properties are stable (boiling point is 195 ℃, solubility in water is 1.5 g/L, log Kow=1.89), the environmental half-life is 72-96 h,LD50>5000 mg/kg (rat oral), and the preparation belongs to GRAS grade safety substances authenticated by FDA. Recent researches show that phenylacetaldehyde can activate bacillus histidine kinase KinD, but a chemotactic recruitment mechanism and application thereof in crop disease prevention and control still remain blank, the invention provides an application of phenylacetaldehyde in improving rice blast resistance, and the phenylacetaldehyde is used as an attractant for preventing and controlling rice blast, thereby providing a new path for preventing and controlling rice blast. Disclosure of Invention The invention aims to provide an application of phenylacetaldehyde in improving rice blast resistance, and the phenylacetaldehyde is used as a small molecule chemotactic agent for improving rice blast resistance, so that a novel approach and means are provided for preventing and treating rice blast. In order to achieve the above purpose, the technical scheme of the invention is as follows: the invention provides application of phenylacetaldehyde in improving rice blast resistance. Preferably, the phenylacetaldehyde is used for guiding bacillus to migrate to the phyllosphere and form a biological film, and the bacillus inhibits rice blast fungus infection through lipopepti